Ctdsp2 (NM_146012) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR200569
Ctdsp2 (Myc-DDK-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (Ctdsp2), transcript variant b
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctdsp2
Synonyms AI586070; D10Ertd73e; OS-4; OS4; SCP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGCTCTTCGAATGTGTTCTCTTCACTGCCAGCTTGGCCAAGTATGCTGACCCTGTGACTGATC
TCCTGGACCGGTGCGGGGTGTTCCGGGCCCGCCTGTTCCGAGAGGCTTGTGTGTTCCACCAGGGCTGCTA
TGTCAAGGACCTCAGCCGCCTGGGAAGGGACCTGAGGAAAACTGTCATCCTGGACAACTCCCCTGCATCT
TACATCTTCCACCCAGAAAATGCAGTGCCTGTGCAGTCCTGGTTTGATGACATGGCAGATACAGAGTTGC
TGAACCTGATTCCAGTCTTCGAGGAGCTAAGTGGAACTGATGATGTCTACACCAGCCTTGGGCAGCTGCG
GGCCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR200569 protein sequence
Red=Cloning site Green=Tags(s)

MGELFECVLFTASLAKYADPVTDLLDRCGVFRARLFREACVFHQGCYVKDLSRLGRDLRKTVILDNSPAS
YIFHPENAVPVQSWFDDMADTELLNLIPVFEELSGTDDVYTSLGQLRAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146012
ORF Size 357 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146012.2, NP_666124.1
RefSeq Size 4136 bp
RefSeq ORF 360 bp
Locus ID 52468
UniProt ID Q8BX07
Cytogenetics 10 74.35 cM
MW 13.4 kDa
Summary Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146012" in other vectors (4)
SKU Description Size Price
MC207494 Ctdsp2 (untagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (Ctdsp2), transcript variant b, (10ug) 10 ug
$165.00
MG200569 Ctdsp2 (tGFP-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (Ctdsp2) 10 ug
$350.00
MR200569L3 Lenti ORF clone of Ctdsp2 (Myc-DDK-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (Ctdsp2), transcript variant b 10 ug
$450.00
MR200569L4 Lenti ORF clone of Ctdsp2 (mGFP-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 (Ctdsp2), transcript variant b 10 ug
$450.00

Plasmid Map for MR200569

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Cloning scheme for MR200569

Circular map for MR200569

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