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Pmvk (BC028659) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200531
Pmvk (Myc-DDK-tagged) - Mouse phosphomevalonate kinase (cDNA clone MGC:41584 IMAGE:1247653)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pmvk
Synonyms 2900002L22Rik, PMK, PMKA, PMKASE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200531 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCTGTTGGGGGGAGCAGAAGCGCCAGGCAGACCCAGGCTTCTTCTGCCGGAAGATTGTGGAAGGCG
TGTCCCAGCCTATCTGGCTGGTGAGTGACACACGGAGGACATCTGACATCCAGTGGTTTCAGGAGGCCTA
TGGGGCTGTGATACAGACAGTCCGAGTAGTGGCCTCGGAGCAGAGTCGACAGCAACGGGGCTGGGTGTTC
ACACCAGGGGTGGACGATGCTGAGTCAGAGTGTGGTCTGGACAACTTTGGGAACTTTGACTGGGTCATTG
AGAACCACGGAGATGAGCAGTGCCTGGAGGATCAGCTGGAGCACCTGCTGGGATTTATTCAAGCCAAACT
T

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200531 protein sequence
Red=Cloning site Green=Tags(s)

MICWGEQKRQADPGFFCRKIVEGVSQPIWLVSDTRRTSDIQWFQEAYGAVIQTVRVVASEQSRQQRGWVF
TPGVDDAESECGLDNFGNFDWVIENHGDEQCLEDQLEHLLGFIQAKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC028659
ORF Size 351 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028659, AAH28659
RefSeq Size 1008 bp
RefSeq ORF 353 bp
Locus ID 68603
Cytogenetics 3 F1
MW 13.4 kDa
Summary Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphosphate and other polyisoprenoid metabolites.UniProtKB/Swiss-Prot Function
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"BC028659" in other vectors (4)
SKU Description Size Price
MC207109 Pmvk (untagged) - Mouse phosphomevalonate kinase (cDNA clone MGC:41584 IMAGE:1247653), (10ug) 10 ug
$165.00
MG200531 Pmvk (tGFP-tagged) - Mouse phosphomevalonate kinase (cDNA clone MGC:41584 IMAGE:1247653) 10 ug
$350.00
MR200531L3 Lenti ORF clone of Pmvk (Myc-DDK-tagged) - Mouse phosphomevalonate kinase (cDNA clone MGC:41584 IMAGE:1247653) 10 ug
$450.00
MR200531L4 Lenti ORF clone of Pmvk (mGFP-tagged) - Mouse phosphomevalonate kinase (cDNA clone MGC:41584 IMAGE:1247653) 10 ug
$450.00

Plasmid Map for MR200531

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200531
Circular map for MR200531

file-alt Citations

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