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Ppdpf (NM_025598) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200479
Ppdpf (Myc-DDK-tagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA 2700038C09 gene (Ppdpf)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppdpf
Synonyms 0610012G23Rik; 2610317A05Rik; 2700038C09Rik; 3110053G12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200479 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCATCCCTTCCAGCGGCTCGCTCGTGGCTACCCATGACTACTATCGGCGACGCCTGGGCTCCT
CGTCCAGCAGCAGCTCCGGCGGAAGTGCCGAGTACCCTGGGGACGCCGTGCTCCAGTCCCCGGGTCTTCC
CAAAGCCGACCCGGGCCACTGGTGGGCCAGCTTCTTTTTCGGGAAATCCACACTCCCGTTCATGACTACA
GTGTTGGAGTCTCCTGAGCGCTCGGCAGAATCCCCCCAGGTCTCCAGAAGCCCGATGACCTGTGGCCTGA
CTCCTGAAACCATGAAGCAGCAGCCAGTCATCCATTCTGGCCAGACCAACCCCAGGGACCTTTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200479 protein sequence
Red=Cloning site Green=Tags(s)

MAAIPSSGSLVATHDYYRRRLGSSSSSSSGGSAEYPGDAVLQSPGLPKADPGHWWASFFFGKSTLPFMTT
VLESPERSAESPQVSRSPMTCGLTPETMKQQPVIHSGQTNPRDLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025598
ORF Size 345 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025598.3
RefSeq Size 797 bp
RefSeq ORF 348 bp
Locus ID 66496
UniProt ID Q9CR37
Cytogenetics 2 H4
MW 12.3 kDa
Summary Probable regulator of exocrine pancreas development.UniProtKB/Swiss-Prot Function
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Documents
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"NM_025598" in other vectors (4)
SKU Description Size Price
MC205094 Ppdpf (untagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA 2700038C09 gene (Ppdpf), (10ug) 10 ug
$150.00
MG200479 Ppdpf (tGFP-tagged) - Mouse RIKEN cDNA 2700038C09 gene (2700038C09Rik) 10 ug
$350.00
MR200479L3 Lenti ORF clone of Ppdpf (Myc-DDK-tagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA 2700038C09 gene (Ppdpf) 10 ug
$450.00
MR200479L4 Lenti ORF clone of Ppdpf (mGFP-tagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA 2700038C09 gene (Ppdpf) 10 ug
$450.00

Plasmid Map for MR200479

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200479
Circular map for MR200479

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