Banf1 (NM_001038231) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR200222
Banf1 (Myc-DDK-tagged) - Mouse barrier to autointegration factor 1 (Banf1), transcript variant 2
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Banf1
Synonyms Baf; Bcrp1; L2bp1/Baf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200222 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAACCTCCCAAAAGCACCGAGACTTCGTGGCAGAGCCCATGGGGGAAAAGCCAGTGGGGAGCCTGG
CCGGGATTGGTGACGTCCTGAGCAAGAGGCTGGAGGAAAGGGGCTTTGACAAGGCTTATGTGGTCCTTGG
CCAGTTTCTGGTGCTAAAGAAAGATGAAGACCTCTTCCGAGAATGGCTGAAGGATACATGTGGTGCCAAT
GCCAAGCAGTCCCGGGACTGCTTTGGGTGCCTTCGAGAATGGTGTGATGCCTTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR200222 protein sequence
Red=Cloning site Green=Tags(s)

MTTSQKHRDFVAEPMGEKPVGSLAGIGDVLSKRLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGAN
AKQSRDCFGCLREWCDAFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001038231
ORF Size 267 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001038231.2
RefSeq Size 766 bp
RefSeq ORF 270 bp
Locus ID 23825
UniProt ID O54962
Cytogenetics 19 A
MW 10.1 kDa
Summary Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001038231" in other vectors (2)
SKU Description Size Price
MR200222L3 Lenti ORF clone of Banf1 (Myc-DDK-tagged) - Mouse barrier to autointegration factor 1 (Banf1), transcript variant 2 10 ug
$450.00
MR200222L4 Lenti ORF clone of Banf1 (mGFP-tagged) - Mouse barrier to autointegration factor 1 (Banf1), transcript variant 2 10 ug
$450.00

Plasmid Map for MR200222

Download sequence for

Cloning scheme for MR200222

Circular map for MR200222

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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