Gng10 (NM_025277) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR200063
Gng10 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 10 (Gng10)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gng10
Synonyms AV006524
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200063 representing NM_025277
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCCGGGGCCAGCGTGAGCGCCCTGCAGCGCCTGGTGGAGCAGCTGAAGCTGGAGGCTGGCGTGG
AGAGGATCAAGGTCTCCCAGGCAGCTGCAGAGCTTCAGCAGTACTGCATACAGAATGCATGCAAGGACGC
CCTGCTGCTCGGTGTTCCGGCTGGAAGCAACCCCTTCCGGGAGCCCAGGTCCTGTGCTCTACTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025277
ORF Size 204 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025277.3, NP_079553.1
RefSeq Size 1120 bp
RefSeq ORF 207 bp
Locus ID 14700
UniProt ID Q9CXP8
Cytogenetics 4 32.36 cM
MW 7.7 kDa
Summary Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Interacts with beta-1 and beta-2, but not with beta-3 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025277" in other vectors (4)
SKU Description Size Price
MC208571 Gng10 (untagged) - Mouse guanine nucleotide binding protein (G protein), gamma 10 (Gng10), (10ug) 10 ug
$165.00
MG200063 Gng10 (tGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 10 (Gng10) 10 ug
$350.00
MR200063L3 Lenti ORF clone of Gng10 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 10 (Gng10) 10 ug
$450.00
MR200063L4 Lenti ORF clone of Gng10 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), gamma 10 (Gng10) 10 ug
$450.00

Plasmid Map for MR200063

Download sequence for

Cloning scheme for MR200063

Circular map for MR200063

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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