Cd86 (NM_019388) Mouse Tagged ORF Clone
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cd86 |
| Synonyms | B7; B7-2; B7.2; B70; Cd28l2; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_019388 |
| ORF Size | 927 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_019388.2 |
| RefSeq Size | 2539 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 12524 |
| UniProt ID | P42082 |
| Cytogenetics | 16 25.72 cM |
| Summary | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_019388" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208265 | Cd86 (untagged) - Mouse CD86 antigen (Cd86), (10ug) | 10 ug |
$450.00
|
|
| MR227055 | Cd86 (Myc-DDK-tagged) - Mouse CD86 antigen (Cd86) | 10 ug |
$450.00
|
|
| MR227055L1 | Lenti ORF clone of Cd86 (Myc-DDK-tagged) - Mouse CD86 antigen (Cd86) | 10 ug |
$750.00
|
|
| MR227055L2 | Lenti ORF clone of Cd86 (mGFP-tagged) - Mouse CD86 antigen (Cd86) | 10 ug |
$750.00
|
|
| MR227055L3 | Lenti ORF clone of Cd86 (Myc-DDK-tagged) - Mouse CD86 antigen (Cd86) | 10 ug |
$750.00
|
|
| MR227055L4 | Lenti ORF clone of Cd86 (mGFP-tagged) - Mouse CD86 antigen (Cd86) | 10 ug |
$750.00
|
|
Citations
Live cell superresolution-structured illumination microscopy imaging analysis of the intercellular transport of microvesicles and costimulatory proteins via nanotubes between immune cells
Halász, Ghadaksaz, Madarász et al
Methods Appl Fluoresc (2018) 6 (4), 045005 DOI: 10.1088/2050-6120/aad57d
Halász, Ghadaksaz, Madarász et al
Methods Appl Fluoresc (2018) 6 (4), 045005 DOI: 10.1088/2050-6120/aad57d
Cited by 27
AI Summary: In this study, OriGene's GFP-CD86 plasmid was used to transfect macrophages and B cells, enabling visualization and analysis of costimulatory protein transport via nanotubes during live cell superresolution imaging.
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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