Col17a1 (NM_007732) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG226746
Col17a1 (tGFP-tagged) - Mouse collagen type XVII alpha 1 (Col17a1), (10ug)
$789.00 $1,426.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Col17a1
Synonyms BP180; Bpag; Bpag2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007732
ORF Size 4299 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007732.2, NP_031758.2
RefSeq Size 5428 bp
RefSeq ORF 4302 bp
Locus ID 12821
UniProt ID Q07563
Cytogenetics 19 40.07 cM
Summary May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.UniProtKB/Swiss-Prot Function
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"NM_007732" in other vectors (4)
SKU Description Size Price
MC224447 Col17a1 (untagged) - Mouse collagen, type XVII, alpha 1 (Col17a1), (10ug) 10 ug
$1,349.00
MR226746 Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$589.00 $1,226.00
MR226746L3 Lenti ORF clone of Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$1,526.00
MR226746L4 Lenti ORF clone of Col17a1 (mGFP-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$1,526.00

Plasmid Map for MG226746

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Cloning scheme for MG226746

Circular map for MG226746

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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