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Col17a1 (NM_007732) Mouse Untagged Clone

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SKU
MC224447
Col17a1 (untagged) - Mouse collagen, type XVII, alpha 1 (Col17a1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,349.00
8 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Col17a1
Synonyms BP180; Bpag; Bpag2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224447 representing NM_007732
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGTGACCAAGAAAAGCAAGCGAGATGGCACTGAAGTCACCGAGAGAATTGTCACGGAAACAGTAA
CGACAAGACTTACATCCCTACCGCCAAAAGGGAGCACCAGCAATGGATATGCTAAGACAGGCTCTCTGGG
TGGAGGAAGTCGGTTGGAGAAGCAGAGCTTGACCCATGGCAGCAGCGGCTACATCAACTCAAGTGGAAGC
ATCCGAGGCAACGCTTCCACCTCCAGTTACAGGAGAACGCACTCACCGGCCTCTACCCTGCCCAACTCTC
CCGGCTCCACCTTTGAAAGGAAAGCTCACATGACCCGCCATGGAACTTATGAAGGGAGCTCTAGCGGCAA
CTCCTCCCCTGAGTACCCACGAAAGGAACTCGCCTCTTCTTCAACCAGAGGACGAAGCCAAACACGAGAG
AGCGAAATTCGAGTTCGGTTGCAGAGCGCGTCGCCATCCACCAGATGGACAGAACTGGATGAGGTGAAGC
GTTTGCTTAAGGGGAGCCGCTCTGCAAGTGCAAGTCCCACCAGGAACACCTCCAACACACTCCCCATCCC
CAAGAAAGGCACCGTAGAGACCAAAACAGTGACAGCGAGCTCCCACTCAGTGTCCGGAACCTATGATTCA
GCAATACTGGACACCAACTTTCCGCCCCACATGTGGTCCTCCACCTTGCCTGCAGGGTCCTCCCTGGGGA
CCTATCAGAACAACATAACAGCCCAGAGCACATCCCTCCTCAACACCAATGCCTACTCAACGGGATCAGT
CTTTGGAGTGCCAAATAACATGGCGTCCTGCTCTCCCACCCTGCACCCCGGACTCAGCAGCTGCTCCTCA
GTGTTTGGCATGCAGAACAATCTGGCCCCCAGCTCTTCTGTCCTCTCCCATGGCACAACCACCGCTTCCA
CAGCATACGGAGCGAAGAAAAACGTGCCCCAGCCTCCCACTGTCACCAGCACCGGCGTGTCCACCTCTGC
CACCTGCACCACCAGTGTCCAGAGTGACGACCTCTTGCATAAAGACTGCAAGTTCCTGATTCTGGAGAAA
GACAACACACCAGCTAAGAAAGAGATGGAACTCTTGATCATGACCAAGGACAGTGGGAAGGTCTTCACTG
CCTCCCCTGCCACCATCTCTTCAACTTCCTTTTCAGAAGACACCCTGAAAAAGGAGAAGCAGGCTGCATA
TGCCGCTGACACCTGCCTGAAGGCAGATGTGAATGGAGACCTAAATACCGTGTCCACAAAGAGCAAGATG
ACCTCGGCAGAAAACCATGGCTACGACCGAGGTGGCGGTGGTGGCAGAGGCAAAGGCGGAGGTGCTGGTG
GTGGCGGTGGTGGCGGTGGCGCCAGTGGCGGTGGAGGAGCATGGGGGGCTGCACCAGCCTGGTGCCCCTG
CGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACCTGGCTGCTGCTGCTGGGTCTG
CTCTTCGGCCTCATTGCTCTGGCGGAGGAGGTAAGAAAGCTGAAGGCCCGCGTGGAGGAGCTGGAAAAGA
CCAAGGTGCTATATCATGACGTCCAGATGGACAAAAGCAACAGGGACCGCCTCCAGGCCGAGGCACCCAG
CCTGGGACCTGGATTAGGCAAGGCTGAGCTGGACGGCTACAGCCAGGAGGCCATCTGGCTGTTTGTAAGG
AACAAGCTGATGACCGAGCAGGAGAACGGGAATCTCAGAGGAAGTCCTGGTCCAAAAGGTGACATGGGGA
GTCAAGGACCTAAAGGAGACCGAGGCCTTCCTGGGACCCCAGGTATCCCTGGGCCCCTGGGCCACCCTGG
CCCGGAAGGACCAAAGGGACAAAAAGGCAGCATTGGAGATCCTGGCATGGAAGGACCCATAGGCCAGAGA
GGACTAGCAGGCCCCATGGGACCTCGTGGTGAACCCGGGCCTCCTGGGTCTGGAGAGAAAGGAGACAGAG
GGATTGCTGGTGAACAAGGTCCTCAGGGCCTTCCTGGTGTCCCAGGTCCTCCGGGTCTCAGAGGTCACAG
CGGCTCTCCTGGTCCCCAAGGCCCCCCAGGTGCTGTGGGTCCCCAAGGGCTCCGAGGTGACGTGGGACTT
CCTGGTGTCAAAGGTGACAAAGGACTCATGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAGGGAC
CCAGAGGCCTCACAGGGGAGCCTGGCATTCGAGGTTTGCCTGGAGCTGTGGGTGAACCCGGAGCCAAAGG
CGCAATGGGTCCGGCTGGCGCTGATGGACAGCAAGGTTCCAGAGGTGAACAAGGCTTGACAGGGATGCCT
GGAACCCGGGGCCCCCCAGGACCCGCTGGAGACCCAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTC
AGGGACTTCCTGGTAGCCCTGGCCGACCAGGGACTAAAGGCGAACCCGGCGCTCCCGGCAGAGTCATGAC
TTCAGAGGGATCATCAACAATCACTGTGCCCGGACCTCCCGGACCTCCTGGTGCCATGGGTCCCCCAGGA
CCTCCAGGGACGCCAGGTCCAGCTGGCCCTGCTGGTCTCCCAGGACAACAAGGCCCACGAGGGGAGCCAG
GACTTGCTGGTGACTCATTCCTAAGCAGTGGCAGCTCCATCTCTGAGGTCCTCTCTGCCCAAGGTGTTGA
CTTACGAGGTCCCCCTGGCCCACCTGGCCCACGAGGGCCACCAGGGCCTTCCATCCCAGGCCCGCCAGGA
CCCAGAGGTCCACCAGGGGAAGGCGTACCAGGCCCACCCGGGCCACCAGGATCCTTCCTGACTGACTCAG
AAACCTTCTTCACTGGCCCTCCAGGTCCACCCGGCCCCCCAGGTCCCAAGGGAGACCAAGGTGATCCTGG
TGTCCCAGGTACTCCTGGCATCTCTGGTGGTCTTTCTCATGGGGCGTCATCAAGCACTCTGTACATGCAG
GGCCCCCCTGGCCCTCCAGGGCCCCCTGGGCCTCCGGGCTCCCTCAGCAGCTCCGGCCAGGATATCCAGC
ACTACATCGCTGAATACATGCAAAGTGACAACATCAGGACTTATCTCTCAGGGGTTCAGGGTCCCCCAGG
CCCACCAGGTCCCCCAGGGCCTGTCATCACCATCACAGGAGAGACTTTCGACTACTCCCAGCTGGCAAGC
CAGGTCGTAAGCTACTTGCGGTCATCAGGCTACGGTGCTGGCTTGTCCTCTGCCTCCTCCTCAGAAGATA
TCTTGGCCATGCTGCGACGGAACGATGTGTGGCAGTACTTACGTCAGAATCTGGTGGGTCCTCCCGGTCC
CCCAGGGCCACCAGGCGTCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGGAGAGCTGAGCAGACAC
ATTCTCAACTATATGTCAAGTTCTGGGATCAGCTTTGGGCATCCTGGCCCACCGGGACCCCCTGGCCTGC
CAGGAACATCCTACGAGGAGCTACTGACCATGCTCCGAGCCGCTGGTTTGTCCTCCATCCCAGGCCCTCC
AGGCCCACCAGGGCCCCCAGGTCCTCGAGGGCCCCCAGGTGTCTCAGCAGCTCTGTCCACCTATGCAGCT
GAGAACAGCGACAACTTCCGCAGCGAGCTGATTAGCTACCTCACAAGTCCTGATGTTCGCAGCTTCATCG
TTGGCCCTCCAGGCCCTCCGGGGCCACAGGGACCACCTGGAGATGGTCACTTAAGGGAGAACTACAACTG
GAGCAGCAACTCCTCAGCCAGAAGGGGCACTTCCTACAGCTCTTCCACGGGCACAGGGGGAACCAACGGA
GGCTCCCTGGGTGAAGGTGGAGCCTATGGTGCAGGAGATGGGGGCCCCTATGGTACCGACATCGGCCCAG
GCGGAGGCTATGGGGCAGCTGCAGGTGGTGGTATATATGGCACCAATGGTGACTCGTTCCGGGATGGCTT
CACTGGAGACCTAGATTACAACAAGCTGGCAGTGCGGGTGTCGGAGAGCATGCAGCGTCAGGGTCTGCTA
CAAGGGATGGCCTACACTGTCCAGGGCCCACCTGGGCCCCAAGGCCCTCCTGGCATCAGCAGGGTCTTCT
CAGCCTACAGCAACGTGACACAAGACCTCATGGACTTCTTCCAAACCTACGGCACTATCCCGGGACCACC
TGGGCAGAAGGGAGATGTGGGAACCCCAGGCCCCAAAGGTGACAGGGGCCCTGCTGGACCACGAGGTCCT
CCAGGGCCACCCGGCCCCAGAGGGAACAAAGGAGAGAAAGGAGACAAAGGTGACCAAGTCTATACTGGGA
GAAGGAAGAGAAGTATTGCCATCAAGCCGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_007732
Insert Size 4302 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007732.2, NP_031758.2
RefSeq Size 5428 bp
RefSeq ORF 4302 bp
Locus ID 12821
UniProt ID Q07563
Cytogenetics 19 40.07 cM
Summary May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_007732" in other vectors (4)
SKU Description Size Price
MG226746 Col17a1 (tGFP-tagged) - Mouse collagen type XVII alpha 1 (Col17a1), (10ug) 10 ug
$789.00 $1,426.00
MR226746 Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$589.00 $1,226.00
MR226746L3 Lenti ORF clone of Col17a1 (Myc-DDK-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$1,526.00
MR226746L4 Lenti ORF clone of Col17a1 (mGFP-tagged) - Mouse collagen, type XVII, alpha 1 (Col17a1) 10 ug
$1,526.00

file-alt Citations

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