Necab2 (NM_054095) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG224122
Necab2 (tGFP-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Necab2
Synonyms Efcbp2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG224122 representing NM_054095
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCGAGCGGGCGGCGCGCCTGTGCAGGGCCGGCGCGCACAGGCTGCTCCGGGAGCCGCCGCCGCAGG
GCCGCGCGCTGGGCGGGCTGCTGCGCTGGGTGGGCGCCAGGATGGGGGAACCCCGTGCACCGCTCGTCCC
CGACATCCCCTCCGCGGACCCCGGCCCCGGGCCCGCCGCATCGCGAGGGGGCACAGCCGTCATCCTGGAC
ATTTTCCGCCGCGCCGATAAAAATGATGATGGGAAGCTGTCCCTGGAGGAGTTTCAACTCTTCTTCGCAG
ATGGCGTCCTTAATGAGAAGGAGCTGGAGGGCCTCTTCCATACCATCGATTCAGACAACACCAACCACGT
GGACACCAAGGAACTGTGTGACTACTTTGTGGAGCACATGGGAGACTATGAAGATGTCCTGGCCTCCCTG
GAGACTTTGAACCACTCAGTCCTGAAGGCCATGGGCTACACTAAGAAGGTGTATGAAGGTGGGAGCAATG
TGGACCAGTTTGTGACACGCTTCCTTCTGAAGGAGACAGCCAATCAGATCCAGTCGCTGCTCAGCTCTGT
GGAAAGTGCTGTAGAAGCCATCGAAGAACAGACCAGCCAGATCCGACAGGACCACTGCAAACCCAGCCAT
GCCGTCAACGAGAGCCGCTACGGAGGCCCTACTCCTCCCTACATCCCCAACCACAAGCTTGTGGCCCCGG
AGCCGATGAAGAGCCTGCCTGTGGCCACAGGGGAGCCAAAGGAGGATGGGCTGGAGGGCCAGATAAGCCG
GTTGGCAGAGCTGATTGGGAGACTGGAGAGCAAGACCCTCTCGTTTGACCTGCAACAGCGCCTCTCGGAT
GAAGAAGGCACCAACATGCACCTGCAGCTGGTCCGGCAGGAAATGGCCGTGTGCCCTGAGCAGCTGAGTG
AGTTCTTGGATTCCCTGAGACAGTACCTCCGGAGCACGGCTGAAGAAAGGAACTGCTTCCATGTTGCCGC
AGTGAGGATGGCAGATGGCCTAACCTTCGTCATCTACGAGTTCTGGGAGACAGAGGAGGAGTGGAAGAGG
CACCTGCAGAGCCCTGTGTGCAAGGCCTTCCGACACGTCAAGGTTGACACGCTGAGCCAGCCTGAAGCAC
TCTCCCAGATCTCCGTGCCAGCTGCCTGGTGCACCTCTGGCCGAGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG224122 representing NM_054095
Red=Cloning site Green=Tags(s)

MCERAARLCRAGAHRLLREPPPQGRALGGLLRWVGARMGEPRAPLVPDIPSADPGPGPAASRGGTAVILD
IFRRADKNDDGKLSLEEFQLFFADGVLNEKELEGLFHTIDSDNTNHVDTKELCDYFVEHMGDYEDVLASL
ETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQIRQDHCKPSH
AVNESRYGGPTPPYIPNHKLVAPEPMKSLPVATGEPKEDGLEGQISRLAELIGRLESKTLSFDLQQRLSD
EEGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRSTAEERNCFHVAAVRMADGLTFVIYEFWETEEEWKR
HLQSPVCKAFRHVKVDTLSQPEALSQISVPAAWCTSGRD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_054095
ORF Size 1167 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054095.2, NP_473436.1
RefSeq Size 1971 bp
RefSeq ORF 1170 bp
Locus ID 117148
UniProt ID Q91ZP9
Cytogenetics 8 E1
Summary May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_054095" in other vectors (4)
SKU Description Size Price
MC212297 Necab2 (untagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2), (10ug) 10 ug
$503.00
MR224122 Necab2 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$289.00 $457.00
MR224122L3 Lenti ORF clone of Necab2 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$757.00
MR224122L4 Lenti ORF clone of Necab2 (mGFP-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$757.00

Plasmid Map for MG224122

Download sequence for

Cloning scheme for MG224122

Circular map for MG224122

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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