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Necab2 (NM_054095) Mouse Untagged Clone

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SKU
MC212297
Necab2 (untagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Necab2
Synonyms Efcbp2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC212297 representing NM_054095
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCGAGCGGGCGGCGCGCCTGTGCAGGGCCGGCGCGCACAGGCTGCTCCGGGAGCCGCCGCCGCAGG
GCCGCGCGCTGGGCGGGCTGCTGCGCTGGGTGGGCGCCAGGATGGGGGAACCCCGTGCACCGCTCGTCCC
CGACATCCCCTCCGCGGACCCCGGCCCCGGGCCCGCCGCATCGCGAGGGGGCACAGCCGTCATCCTGGAC
ATTTTCCGCCGCGCCGATAAAAATGATGATGGGAAGCTGTCCCTGGAGGAGTTTCAACTCTTCTTCGCAG
ATGGCGTCCTTAATGAGAAGGAGCTGGAGGGCCTCTTCCATACCATCGATTCAGACAACACCAACCACGT
GGACACCAAGGAACTGTGTGACTACTTTGTGGAGCACATGGGAGACTATGAAGATGTCCTGGCCTCCCTG
GAGACTTTGAACCACTCAGTCCTGAAGGCCATGGGCTACACTAAGAAGGTGTATGAAGGTGGGAGCAATG
TGGACCAGTTTGTGACACGCTTCCTTCTGAAGGAGACAGCCAATCAGATCCAGTCGCTGCTCAGCTCTGT
GGAAAGTGCTGTAGAAGCCATCGAAGAACAGACCAGCCAGATCCGACAGGACCACTGCAAACCCAGCCAT
GCCGTCAACGAGAGCCGCTACGGAGGCCCTACTCCTCCCTACATCCCCAACCACAAGCTTGTGGCCCCGG
AGCCGATGAAGAGCCTGCCTGTGGCCACAGGGGAGCCAAAGGAGGATGGGCTGGAGGGCCAGATAAGCCG
GTTGGCAGAGCTGATTGGGAGACTGGAGAGCAAGACCCTCTCGTTTGACCTGCAACAGCGCCTCTCGGAT
GAAGAAGGCACCAACATGCACCTGCAGCTGGTCCGGCAGGAAATGGCCGTGTGCCCTGAGCAGCTGAGTG
AGTTCTTGGATTCCCTGAGACAGTACCTCCGGAGCACGGCTGAAGAAAGGAACTGCTTCCATGTTGCCGC
AGTGAGGATGGCAGATGGCCTAACCTTCGTCATCTACGAGTTCTGGGAGACAGAGGAGGAGTGGAAGAGG
CACCTGCAGAGCCCTGTGTGCAAGGCCTTCCGACACGTCAAGGTTGACACGCTGAGCCAGCCTGAAGCAC
TCTCCCAGATCTCCGTGCCAGCTGCCTGGTGCACCTCTGGCCGAGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_054095
Insert Size 1170 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054095.2, NP_473436.1
RefSeq Size 1971 bp
RefSeq ORF 1170 bp
Locus ID 117148
UniProt ID Q91ZP9
Cytogenetics 8 E1
Summary May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.UniProtKB/Swiss-Prot Function
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"NM_054095" in other vectors (4)
SKU Description Size Price
MG224122 Necab2 (tGFP-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2), (10ug) 10 ug
$657.00
MR224122 Necab2 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$289.00 $457.00
MR224122L3 Lenti ORF clone of Necab2 (Myc-DDK-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$757.00
MR224122L4 Lenti ORF clone of Necab2 (mGFP-tagged) - Mouse N-terminal EF-hand calcium binding protein 2 (Necab2) 10 ug
$757.00

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