Akap1 (NM_009648) Mouse Tagged ORF Clone

SKU MG222048

Akap1 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 1 (Akap1) nuclear gene encoding mitochondrial protein transcript variant 1, (10ug)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Akap1
• Synonyms: Akap; AKAP84; AKAP121; C76494; C81186; DAKAP1; S-AKAP84
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222048 representing NM_009648; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAATCCAGTTGCGTTCGCTCTTCCCCTTGGCGTTGCCCGGAATGCTGGCCCTCCTTGGCTGGTGGT; GGTTTTTCTCTCGTAAAAAAGATCGGCTCAGCAGCAGTGATAAGCAGGTGGAGACACTGAAGGTTGGCCC; TGCCATCAAGGACCGACGGCTCAGTGAAGAGGCCTGTCCTGGAGTCCTGTCTGTGGCCCCCACTGTCACA; CAGCCTCCTGGAAGGGAAGAGCAGCGCTGTGTGGACAAGCCTTCTACAGAGCCCCTGGCCTTGCCGAGGA; CTCGCCAGGTTCGACGAAGATCAGAGTCCTCAGGCAACCTCCCCAGCGTTGCAGACACGAGGTCGCAGCC; AGGACCGTGCAGAGATGAGATCGCCAAAGTGGAACTCTCCCTGATGGGGGACAAAGCCAAATCTATTCCT; CTTGGATGTCCGCTTCTCCCAAAGGATGCGTCCTTCCCCTATGAAGCAGTGGAAAGGTGTAAGCAGGAGT; CCGCACTGGGCAAGACTCCTGGAAGAGGCTGGCCAAGCCCGTATGCGGCCTCTGGAGAGAAAGCGAGAGA; GACAGGTGGGACAGAGGGGACTGGAGATGCTGTGTTGGGGGAAAATGTATCTGAGGAAGGCCTATTGTCC; CAGGAGTGTGTCTCAGAAGTGGAGAAGAGTGAGTTTCCAATCCTGGCCCCCGGGGGAGGTGAGGGAGAAG; AGGTGAGCCATGGCCCCCCACAGGTAGCTGAACTTTTAAAGAAGGAAGAATATATTGTTGGGAAGTTGCC; GAGTAGCTTTGTGGAGCCAGTTCACTCAGAGCCGGTTAAGGACGAGGATGCGTTGGAACCCCAGGTCAAA; GGTAGCAGCAATACTTCGGATAGAGACCTGGCTGGAGAGCTGGACAAAGACGAGACCGTGCCTGAAAATG; ACCAGATTAAGCAGGCTGCCTTCCAGCTCATCTCCCAGGTGATCTTGGAAGCAACTGAAGAGTTTCGGGC; CACCACAGTGGGCAAGACTGTGGCACAAGTGCACCCAACCTCGGCCACTCAGCCTAAGGGGAAGGAGGAG; AGCTGTGTTCCAGCCAGCCAGGAAACTAGCTTGGGACAAGACACCTCAGATCCTGCTTCCACCAGAACAG; GTGCCACTGCCAGCCCTTCAGCAGAAGCTCTGCCACCAAAGACCTATGTAAGCTGTCTCAGCAGCCCTCT; GTCAGGCCCCACCAAGGACCAGAAGCCAAAGAACTCTGCACATCACATCTCCCTGGCTCCCTGCCCACCG; CCAGTCACCCCCCAGAGGCAGTCTCTGGAGGGGGCAAGTAACCCGAGAGGTGATGACAACTTTGTCGCCT; GTATGGCCAACAACAGCCAGAGTGTCCTTTCAGTTAGCTCCTTGGGGCAGTGCTCAGATCCTGTCAGTAC; TTCGGGGCTTGAAGACTCTTGCACAGAGACCATCTCAAGCTCCGGAGACAAAGCTATGACCCCACCACTG; CCAGTCAGTACTCAGCCCTTCAGCAACGGGGTGCTGAAGGAGGAGCTGTCAGACTTAGGGACCGAGGATG; GATGGACCATGGATACAGAAGCAGATCACTCAGGAGGTTCTGACGGGAACAGTATGGATTCAGTGGATAG; CTGTTGCGGGCTTACCAAGCCCGATAGCCCCCAGAGTGTCCAGGCAGGCTCCAACCCTAAGAAGGTTGAC; CTTATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTTGGTCGACTGATTGGCAAGCAGGGACGGTACG; TGAGTTTTCTGAAGCAGACATCTGGTGCCAAGATCTACATCTCCACCCTGCCTTACACACAGAACATCCA; GATCTGCCACATAGAAGGCTCTCAGCACCATGTAGACAAAGCTCTGAACTTGATTGGGAAGAAGTTTAAG; GAACTGAACCTCACCAATATCTATGCGCCACCACTGCCTTCGCTGGCACTGCCTTCCTTGCCGATGACGT; CTTGGCTCATGCTGCCTGATGGTATCACTGTGGAAGTCATCGTGGTCAACCAGGTCAATGCTGGGCACCT; ATTTGTCCAGCAGCACACACACCCCACCTTCCATGCACTGCGCAGTCTGGACCAGCAGATGTACCTCTGT; TACTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATCACGGTTATCTGCGCTGCCCCTGGTG; CGGACGGGGCCTGGTGGCGAGCCCAAGTAGTGGCTTCCTATGAGGAGACCAATGAGGTGGAGATTCGCTA; CGTGGACTATGGTGGATATAAGAGAGTGAAAGTCGACGTGCTCCGGCAAATTAGGTCTGACTTTGTGACC; CTGCCATTCCAAGGAGCAGAAGTCCTTCTGGACAGTGTGGTTCCTCTGTCAGATGATGATCATTTTTCAC; CGGAGGCAGACGCAGCCATGAGTGAGATGACAGGCAATACAGCACTGTTGGCCCAGGTGACAAGCTACAG; TGCGACTGGCCTTCCTCTGATTCAGCTATGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCG; CTGGTGGAGCGAGGCCTTGCACAGTGGGTAGACAGCTACTATGCCAGCCTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222048 representing NM_009648; Red=Cloning site Green=Tags(s); ; MAIQLRSLFPLALPGMLALLGWWWFFSRKKDRLSSSDKQVETLKVGPAIKDRRLSEEACPGVLSVAPTVT; QPPGREEQRCVDKPSTEPLALPRTRQVRRRSESSGNLPSVADTRSQPGPCRDEIAKVELSLMGDKAKSIP; LGCPLLPKDASFPYEAVERCKQESALGKTPGRGWPSPYAASGEKARETGGTEGTGDAVLGENVSEEGLLS; QECVSEVEKSEFPILAPGGGEGEEVSHGPPQVAELLKKEEYIVGKLPSSFVEPVHSEPVKDEDALEPQVK; GSSNTSDRDLAGELDKDETVPENDQIKQAAFQLISQVILEATEEFRATTVGKTVAQVHPTSATQPKGKEE; SCVPASQETSLGQDTSDPASTRTGATASPSAEALPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPP; PVTPQRQSLEGASNPRGDDNFVACMANNSQSVLSVSSLGQCSDPVSTSGLEDSCTETISSSGDKAMTPPL; PVSTQPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQSVQAGSNPKKVD; LIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFK; ELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLC; YSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVT; LPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRS; LVERGLAQWVDSYYASL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009648
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009648.2, NP_033778.2
• RefSeq Size: 3721 bp
• RefSeq ORF: 2574 bp
• Locus ID: 11640
• UniProt ID: O08715
• Cytogenetics: 11 C
• Summary: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.[UniProtKB/Swiss-Prot Function]