Akap1 (NM_009648) Mouse Untagged Clone

SKU MC222137

Akap1 (untagged) - Mouse A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Akap1
• Synonyms: Akap; AKAP84; AKAP121; C76494; C81186; DAKAP1; S-AKAP84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222137 representing NM_009648; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAATCCAGTTGCGTTCGCTCTTCCCCTTGGCGTTGCCCGGAATGCTGGCCCTCCTTGGCTGGTGGT; GGTTTTTCTCTCGTAAAAAAGATCGGCTCAGCAGCAGTGATAAGCAGGTGGAGACACTGAAGGTTGGCCC; TGCCATCAAGGACCGACGGCTCAGTGAAGAGGCCTGTCCTGGAGTCCTGTCTGTGGCCCCCACTGTCACA; CAGCCTCCTGGAAGGGAAGAGCAGCGCTGTGTGGACAAGCCTTCTACAGAGCCCCTGGCCTTGCCGAGGA; CTCGCCAGGTTCGACGAAGATCAGAGTCCTCAGGCAACCTCCCCAGCGTTGCAGACACGAGGTCGCAGCC; AGGACCGTGCAGAGATGAGATCGCCAAAGTGGAACTCTCCCTGATGGGGGACAAAGCCAAATCTATTCCT; CTTGGATGTCCGCTTCTCCCAAAGGATGCGTCCTTCCCCTATGAAGCAGTGGAAAGGTGTAAGCAGGAGT; CCGCACTGGGCAAGACTCCTGGAAGAGGCTGGCCAAGCCCGTATGCGGCCTCTGGAGAGAAAGCGAGAGA; GACAGGTGGGACAGAGGGGACTGGAGATGCTGTGTTGGGGGAAAATGTATCTGAGGAAGGCCTATTGTCC; CAGGAGTGTGTCTCAGAAGTGGAGAAGAGTGAGTTTCCAATCCTGGCCCCCGGGGGAGGTGAGGGAGAAG; AGGTGAGCCATGGCCCCCCACAGGTAGCTGAACTTTTAAAGAAGGAAGAATATATTGTTGGGAAGTTGCC; GAGTAGCTTTGTGGAGCCAGTTCACTCAGAGCCGGTTAAGGACGAGGATGCGTTGGAACCCCAGGTCAAA; GGTAGCAGCAATACTTCGGATAGAGACCTGGCTGGAGAGCTGGACAAAGACGAGACCGTGCCTGAAAATG; ACCAGATTAAGCAGGCTGCCTTCCAGCTCATCTCCCAGGTGATCTTGGAAGCAACTGAAGAGTTTCGGGC; CACCACAGTGGGCAAGACTGTGGCACAAGTGCACCCAACCTCGGCCACTCAGCCTAAGGGGAAGGAGGAG; AGCTGTGTTCCAGCCAGCCAGGAAACTAGCTTGGGACAAGACACCTCAGATCCTGCTTCCACCAGAACAG; GTGCCACTGCCAGCCCTTCAGCAGAAGCTCTGCCACCAAAGACCTATGTAAGCTGTCTCAGCAGCCCTCT; GTCAGGCCCCACCAAGGACCAGAAGCCAAAGAACTCTGCACATCACATCTCCCTGGCTCCCTGCCCACCG; CCAGTCACCCCCCAGAGGCAGTCTCTGGAGGGGGCAAGTAACCCGAGAGGTGATGACAACTTTGTCGCCT; GTATGGCCAACAACAGCCAGAGTGTCCTTTCAGTTAGCTCCTTGGGGCAGTGCTCAGATCCTGTCAGTAC; TTCGGGGCTTGAAGACTCTTGCACAGAGACCATCTCAAGCTCCGGAGACAAAGCTATGACCCCACCACTG; CCAGTCAGTACTCAGCCCTTCAGCAACGGGGTGCTGAAGGAGGAGCTGTCAGACTTAGGGACCGAGGATG; GATGGACCATGGATACAGAAGCAGATCACTCAGGAGGTTCTGACGGGAACAGTATGGATTCAGTGGATAG; CTGTTGCGGGCTTACCAAGCCCGATAGCCCCCAGAGTGTCCAGGCAGGCTCCAACCCTAAGAAGGTTGAC; CTTATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTTGGTCGACTGATTGGCAAGCAGGGACGGTACG; TGAGTTTTCTGAAGCAGACATCTGGTGCCAAGATCTACATCTCCACCCTGCCTTACACACAGAACATCCA; GATCTGCCACATAGAAGGCTCTCAGCACCATGTAGACAAAGCTCTGAACTTGATTGGGAAGAAGTTTAAG; GAACTGAACCTCACCAATATCTATGCGCCACCACTGCCTTCGCTGGCACTGCCTTCCTTGCCGATGACGT; CTTGGCTCATGCTGCCTGATGGTATCACTGTGGAAGTCATCGTGGTCAACCAGGTCAATGCTGGGCACCT; ATTTGTCCAGCAGCACACACACCCCACCTTCCATGCACTGCGCAGTCTGGACCAGCAGATGTACCTCTGT; TACTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATCACGGTTATCTGCGCTGCCCCTGGTG; CGGACGGGGCCTGGTGGCGAGCCCAAGTAGTGGCTTCCTATGAGGAGACCAATGAGGTGGAGATTCGCTA; CGTGGACTATGGTGGATATAAGAGAGTGAAAGTCGACGTGCTCCGGCAAATTAGGTCTGACTTTGTGACC; CTGCCATTCCAAGGAGCAGAAGTCCTTCTGGACAGTGTGGTTCCTCTGTCAGATGATGATCATTTTTCAC; CGGAGGCAGACGCAGCCATGAGTGAGATGACAGGCAATACAGCACTGTTGGCCCAGGTGACAAGCTACAG; TGCGACTGGCCTTCCTCTGATTCAGCTATGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCG; CTGGTGGAGCGAGGCCTTGCACAGTGGGTAGACAGCTACTATGCCAGCCTCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009648
• Insert Size: 2574 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009648.2, NP_033778.2
• RefSeq Size: 3721 bp
• RefSeq ORF: 2574 bp
• Locus ID: 11640
• UniProt ID: O08715
• Cytogenetics: 11 C
• Summary: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum. Does not contain the classic KDEL endoplasmic reticulum-targeting sequence. This explains how it is able to switch its localization, either being in the endoplasmic reticulum or in the mitochondria depending on which N-terminal part begins the isoform. The longest N-terminal part only present in isoform 2 and isoform 4 acts as a suppressor of mitochondrial targeting and as an activator of recessive endoplasmic reticulum targeting motif.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) and variant 2 encode the same protein.