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Mrgpra4 (NM_153524) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216115
Mrgpra4 (tGFP-tagged) - Mouse MAS-related GPR member A4 (Mrgpra4), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrgpra4
Synonyms Mgrg10; MrgA4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216115 representing NM_153524
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACCCACAACAACAAATCCAATGAACGAAACCATCCCTGGAAGTATTGACATCGAGACCCTGATCC
CAAACTTGATGATCATCATCTTCGGACTGGTCGGGCTGACAGGAAATGTCATTTTGTTTTGGCTCCTGGG
CTTCCACTTGCACAGGAATGCCTTCTTAGTCTACATCCTAAACTTGGCCCTGGCTGACTTCCTCTTCCTT
CTCTGTCACATCATAAATTCCACAATGCTTCTTCTCAAGGTTCACCTACCCAACAATATTTTGAACCATT
GCTTTGACATCATCATGACAGTTCTCTACATCACAGGCCTGAGCATGCTCAGTGCCATCAGCACTGAGCG
CTGCCTGTCTGTCCTGTGCCCCATCTGGTATCGGTGCCGCCGCCCAGAACACACATCAACTGTCCTGTGT
GCTGTGATCTGGTTCCTGCCCCTGTTGATCTGCATTCTGAATGGATATTTCTGTCATTTCTTTGGTCCCA
AATATGTAATTGACTCTGTGTGTCTGGCAACGAACTTCTTTATCAGAACATACCCGATGTTTTTGTTTAT
AGTCCTCTGTCTGTCCACCCTGGCTCTGCTGGCCAGGTTGTTCTGTGGTGCTGGGAAGACGAAATTTACC
AGATTATTCGTGACCATCATGCTGACCGTTTTGGTTTTTCTTCTCTGTGGGTTGCCCCTGGGCTTCTTCT
GGTTTCTGGTGCCCTGGATTAACCGTGATTTCAGTGTACTAGATTATATACTTTTTCAGACATCACTTGT
CCTAACTTCTGTTAACAGCTGTGCCAACCCCATCATTTACTTCTTTGTGGGCTCCTTCAGGCATCGGTTG
AAGCACAAGACCCTCAAAATGGTTCTCCAGAGTGCATTGCAGGACACTCCTGAGACACCTGAAAACATGG
TGGAGATGTCAAGAAGCAAAGCAGAGCCG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216115 representing NM_153524
Red=Cloning site Green=Tags(s)

MAPTTTNPMNETIPGSIDIETLIPNLMIIIFGLVGLTGNVILFWLLGFHLHRNAFLVYILNLALADFLFL
LCHIINSTMLLLKVHLPNNILNHCFDIIMTVLYITGLSMLSAISTERCLSVLCPIWYRCRRPEHTSTVLC
AVIWFLPLLICILNGYFCHFFGPKYVIDSVCLATNFFIRTYPMFLFIVLCLSTLALLARLFCGAGKTKFT
RLFVTIMLTVLVFLLCGLPLGFFWFLVPWINRDFSVLDYILFQTSLVLTSVNSCANPIIYFFVGSFRHRL
KHKTLKMVLQSALQDTPETPENMVEMSRSKAEP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153524
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153524.2, NP_705744.2
RefSeq Size 1503 bp
RefSeq ORF 942 bp
Locus ID 235854
UniProt ID Q91WW2
Cytogenetics 7 B4
Summary Orphan receptor. May be a receptor for RFamide-family neuropeptides such as NPFF and NPAF, which are analgesic in vivo. May regulate nociceptor function and/or development, including the sensation or modulation of pain.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153524" in other vectors (4)
SKU Description Size Price
MC213013 Mrgpra4 (untagged) - Mouse MAS-related GPR, member A4 (Mrgpra4), (10ug) 10 ug
$300.00
MR216115 Mrgpra4 (Myc-DDK-tagged) - Mouse MAS-related GPR, member A4 (Mrgpra4) 10 ug
$289.00 $300.00
MR216115L3 Lenti ORF clone of Mrgpra4 (Myc-DDK-tagged) - Mouse MAS-related GPR, member A4 (Mrgpra4) 10 ug
$600.00
MR216115L4 Lenti ORF clone of Mrgpra4 (mGFP-tagged) - Mouse MAS-related GPR, member A4 (Mrgpra4) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.