Khdrbs2 (NM_133235) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215998
Khdrbs2 (tGFP-tagged) - Mouse KH domain containing RNA binding signal transduction associated 2 (Khdrbs2), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Khdrbs2
Synonyms 6330586C16Rik; mSLM-1; Slim1; SLM; Slm-1; Slm1; Tg(LRRK2*R1441G)135Cjli; TG-RP135
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215998 representing NM_133235
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGAAGAGAAATACTTGCCTGAGCTGATGGCAGAGAAGGATAGCCTGGATCCATCTTTTGTGCACG
CGTCGCGCCTTCTGGCGGAAGAAATTGAGAAATTTCAAGGTTCAGATGGGAAAAAGGAAGATGAAGAAAA
GAAATATCTCGATGTCATCAGCAACAAAAACATAAAGCTCTCTGAAAGAGTATTGATTCCTGTGAAACAG
TATCCAAAGTTCAATTTTGTGGGGAAATTGCTTGGACCAAGAGGAAACTCCTTGAAGAGGCTACAAGAAG
AAACGGGTGCTAAAATGTCTATCCTGGGCAAAGGGTCCATGCGAGATAAGACAAAGGAAGAAGAGCTGAG
GAAGAGTGGGGAGGCCAAGTATGCCCACCTGAGTGATGAGCTGCATGTATTAATTGAAGTGTTTGCTCCA
CCCGGGGAAGCTTATTCACGGATGAGTCATGCCTTGGAAGAGATTAAAAAATTCCTGGTTCCTGACTACA
ATGATGAAATTCGTCAAGAGCAACTCCGGGAGTTGTCTTACTTGAATGGCTCAGAAGAGTCTGGCCGGGG
CCGAGGTATTAGAGGCAGAGGGATCAGAATAACTCCCACAGCTCCATCAAGGGGCCGTGGCGGTGCTGTT
CCACCACCACCACCACCTGGACGAGGTGTGCTTACCCCTCGGGGGACCACTGTGACCCGTGGAGCTCTTC
CAGTGCCCCCAATAGCAAGAGGTGTCCCCACACCTCGAGCCCGGGGGACGGCAGCAGTACCAGGATACAG
AGCACCCCCACCTCCAGCTCATGATGCTTATGAAGAATATGGGTATGATGATGGCTATGGGGGTGAATAT
GATGACCAGACCTATGAGGCTTATGATAATAGCTACGTGACCCCAACACAAAGTGTGCCTGAATACTATG
ACTACGGTCATGGAGTAAACGAGGATGCCTACGACAGCTACGCACCAGAAGAATGGGCCACAACTCGCTC
CAGCCTGAAGGCACCACCACCAAGGTCAGCCAGAGGGGGATACAGGGAGCACCCCTATGGTAGATAT


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215998 representing NM_133235
Red=Cloning site Green=Tags(s)

MGEEKYLPELMAEKDSLDPSFVHASRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQ
YPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKTKEEELRKSGEAKYAHLSDELHVLIEVFAP
PGEAYSRMSHALEEIKKFLVPDYNDEIRQEQLRELSYLNGSEESGRGRGIRGRGIRITPTAPSRGRGGAV
PPPPPPGRGVLTPRGTTVTRGALPVPPIARGVPTPRARGTAAVPGYRAPPPPAHDAYEEYGYDDGYGGEY
DDQTYEAYDNSYVTPTQSVPEYYDYGHGVNEDAYDSYAPEEWATTRSSLKAPPPRSARGGYREHPYGRY

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_133235
ORF Size 1047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133235.3
RefSeq Size 1363 bp
RefSeq ORF 1050 bp
Locus ID 170771
UniProt ID Q9WU01
Cytogenetics 1 B
Summary The protein encoded by this gene is similar to the src associated in mitosis, 68 kDa protein, which is an RNA-binding protein and a substrate for Src-family tyrosine kinases during mitosis. This protein has a KH RNA-binding motif and proline-rich motifs which may be SH2 and SH3 domain binding sites. A similar rat protein is an RNA-binding protein which is tyrosine phosphorylated by Src during mitosis. These studies also suggest that the rat protein may function as an adaptor protein for Src by binding the SH2 and SH3 domains of various other proteins. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_133235" in other vectors (4)
SKU Description Size Price
MC212336 Khdrbs2 (untagged) - Mouse KH domain containing, RNA binding, signal transduction associated 2 (Khdrbs2), (10ug) 10 ug
$503.00
MR215998 Khdrbs2 (Myc-DDK-tagged) - Mouse KH domain containing, RNA binding, signal transduction associated 2 (Khdrbs2) 10 ug
$289.00 $457.00
MR215998L3 Lenti ORF clone of Khdrbs2 (Myc-DDK-tagged) - Mouse KH domain containing, RNA binding, signal transduction associated 2 (Khdrbs2) 10 ug
$757.00
MR215998L4 Lenti ORF clone of Khdrbs2 (mGFP-tagged) - Mouse KH domain containing, RNA binding, signal transduction associated 2 (Khdrbs2) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.