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A830010M20Rik (NM_001007574) Mouse Tagged ORF Clone

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SKU
MG215681
A830010M20Rik (tGFP-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,147.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "A830010M20Rik or NM_001007574"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol A830010M20Rik
Synonyms AI849226; Kiaa1107; mKIAA1107
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215681 representing NM_001007574
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTTAGATGATCAGCAGAAAATTCAAGTAGCTGCACTTGACAAAGGTGATGACCGCAGACTTGGCA
GGAAGCCTGTGCTCACCAGCTCTCAGCAAAGGAGACAAGGCTCTGATGTTGATGTTTTAAAAATAAAACC
TTGGACAGAAAATAACAAAAAACCGTGTCAGACCTCTCTCTCTACTAATCAGAAGATGAGATCCGATGGA
TTGGGAGCATCAGGACATGCATCAAGCACTAACAGAAACAGTATAAATAAAGTGTCCAAGCATGGTGACA
GTACAAAAATGTCCAAAGTTGTGAAAGAGATGAAAACTGGGGGAAAATATGTCTCTGGCAAGTCTAAAAC
TATGGTAAAGCCCCAAACAGAAAACAGTGATCATACAAAGATAGAAGGTTTGTCATCCACTGTTGTGGGA
AGACCATCAAGAGTGACAGCAGCTGGACGGAAGGACCCAGTACATGGAAAAGGAGTCCAGAACCAAGAAG
TGGAGACCACAGGGGCAAGACCTAAGGTACTCACTGCAAACCTGAACGTGCAAGCCAGAGCCAAGCCTTT
GCAAACACTGAGAGGAAAAGATTCCACATGCCCCGCCTCTGTAGGGCCCTCCAGTAGATCTACACATTCC
AGCACGGAGCTTCTGGCTTCCATGGGCTCTGTGGATGAGACAAAGGAAAACGGATCAGTAGAAGACAAGT
GTCGTGATGGGAAGCCATACGTTAGTGATTCCCCAGGACAGATGGTGAGCAATGGAGTAATGTCAACTGC
AGCAGTAAAGTCTCGAGCAGTTGCAAGAATTACCAATGGAACAGCCAGTAACAAAAGCTTTACTCATGAA
CAAGACTCTCACGGGAATAGCAGTGTAATAAAAAGAGGGGATGGCAAAGGACTTAGTGCTTCAGCACCAC
AGACAGCTGCGAAGAAAAGAGGGAGCAGCAATGGATGCACAGCAGCTCAGCCACGGACGAAGAGCGCCCC
TCCCACCCTGGCTCAAGCTCAAGGATCCCAAGGGGAGTCACCACACTCTGTAAAATCTTCAGTCTCTTCA
AGGCAGTCTGATGAAAATGTAACAAGGTTGAGGCATGGAACAGATAAGCAAATACCTAAGAGAAAAGTTG
TCAAGCAAGGACACACAACTTTGCAGAAGGTTAATGCAAAGCTGGTACCAATGCCTAAAATCCCAAGTCA
GCCCAAGAAAGGTGGAACTGTAAACAGCAAAGATTCAAAACAAAAAGTGCTTCCTGGACAAATTATGCTG
CAAAGTCATGCTTCTCAGCGACCTTCCAAACCTGAGGTAGCTGAGAAAAGCGTGCTTCATGGTGTGAGTG
ACAGCAGGAACCATGTCTCTAAACAGAGGCCTCACGAGTCCCCGTGTACCCTTGCTTGTGACACCAGTGG
TCCTGAAGTGTCCCAGATACCTTGCAGACCTCAGCCACAAAGTCCATTAGGCAGTCAAGAGAAAAAGAGA
CTGGAGCTAGCATGTCAAGATAGGTCAACCCTGGGCGACTCAGTGAAACGTGAGCTGAGGTCAGAACTGG
CTGGCATAGAGCAAAGTCACACATCCGCTTACAAAGACTCGAGTCAGTGCAATGGGAATCCAAACTGTGG
GAGCATTGCTGCTCTGAAATCTGTGATTTCAAATCCAAATGAAAACTTGATAAACTCTAACCCAGTTCAT
AATTCAGACTCAGCAAATACAGAGCAAGCCTATTTATCAGATAGAGAGAGAGAAACAGGGAGAGAAAACA
CAGATACTGAACCAAGTATGAGTTGTATAAATGGGAAAGCTTTCCTGTGTGTTCCTGAAGGGACAAGCAG
TACCCTCAACCCTACTCAAAGTGACAGAAGACTCACATTTCATGGAGCAGCTCAAAGCCAGTTACCTGAC
GACTGTGCTACAGAGGACAGTAAATGTGCTACAGTCACCTCAGCTGTTTCTTCCAAATGTCTTTTGGGAC
AACCATCAGAAAAAAATTGTAAAAATATGGAAATGTCAGAAACCCCAGAGAGCCATGGAACTCCAGAAGC
TCCATTCGTGGGCCCCTGGGATTTGAACACTGGTGCCACACATCAAAGAGAGAGTCCAGAATCCGACACT
GGTAGTGCTACCACATCTTCAGACGACATAAAGCCCCGCTCAGAAGACTATGATGCTGGGGGGTCTCAGG
ATGATGAGGGCTCACACGACAGAGGCATCTCCAAGTGCAGCACAGCGCTGTGCCATGACTTCCTTGGGAG
GAGCAGCAGCGACACCAGCACCCCTGAAGAGTTGAAGGTGCACGAGGGTGACCTCAGGACTGAAGTCAGA
GTGAGAAAGCAGGGTGGGGGTGACCACCAGATCCACTCGGCAAGTGATGACGAGATCCCTAGGAAGAAGC
CAGAACCTTGGTCTCGATCTACAATAGGCTATCCCCGGGAGAAGGGAAGTACGCCACGGGGTAGCATCCC
ATCTGCTCAGGAAGGGGACCAGGTTTCTTCTTCAGCAGATGAAACAGAAGATGAAAGATCTGAAGCAGAG
AATGTTGGGGAAAATTCCTCTCCATGCAGCTCAGGTACTCAGCAGGTTCAGGGAATAATTAACTTAGCTT
TTGACGATGGAGCTGAACATGAAAGTCGTGAGTTTTCTGCAACTAAGAAGTTTAGAAGGTCAGTGTTGCT
CTCGGTAGATGAGTGTGAAGAAGTGGGATCTGATGAAGGTGAAGGCCACACCCCCTTTCAGCCTTCCCTA
GATTCTCTTTCACCTTCTGATGTTTTTGATGGTGTTTCTTATGAGCATCATGGAAGGACAGGTTACTCTA
GATTTTTCAAAGAAAATGAAGCTACTACTGCAGAGCATAATCACAACAAAGGCAATGGTGGATATAAAAA
TGAAAGCTTTCTCCTGAATTCTAGAAGCAAAGACTTCGAAAAACAAGATAAACAATGTGTCTCAACAGAT
CAAAAGACCAGGTTAGATGTCCCACCCAAAGGAAGCCAACAGCTTTTTCCGGAAAACGAAGAAGTGATAA
GTAAACGGGAGGCGGCTCACAGCTTTCAGCAGCACAGCACATTCATGGATGGGGACACTAAGTCTCAAGA
ACGACCGTGTCATTTGGAGCTCCATCAGAGAGAACTCAGCTCCAATATACCAAAGATCAGCTCAGTGAAA
TCTCTGGACTCCTGTCCCAGCCAGGGGCTGCCTCAGGAAGGGCAAGTGAAAGAGAGCCGTCCCACGCCTC
CCAAGGGAGCTAATAACGCTGTGTTCTCAGGTAATGTACAGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215681 representing NM_001007574
Red=Cloning site Green=Tags(s)

MHLDDQQKIQVAALDKGDDRRLGRKPVLTSSQQRRQGSDVDVLKIKPWTENNKKPCQTSLSTNQKMRSDG
LGASGHASSTNRNSINKVSKHGDSTKMSKVVKEMKTGGKYVSGKSKTMVKPQTENSDHTKIEGLSSTVVG
RPSRVTAAGRKDPVHGKGVQNQEVETTGARPKVLTANLNVQARAKPLQTLRGKDSTCPASVGPSSRSTHS
STELLASMGSVDETKENGSVEDKCRDGKPYVSDSPGQMVSNGVMSTAAVKSRAVARITNGTASNKSFTHE
QDSHGNSSVIKRGDGKGLSASAPQTAAKKRGSSNGCTAAQPRTKSAPPTLAQAQGSQGESPHSVKSSVSS
RQSDENVTRLRHGTDKQIPKRKVVKQGHTTLQKVNAKLVPMPKIPSQPKKGGTVNSKDSKQKVLPGQIML
QSHASQRPSKPEVAEKSVLHGVSDSRNHVSKQRPHESPCTLACDTSGPEVSQIPCRPQPQSPLGSQEKKR
LELACQDRSTLGDSVKRELRSELAGIEQSHTSAYKDSSQCNGNPNCGSIAALKSVISNPNENLINSNPVH
NSDSANTEQAYLSDRERETGRENTDTEPSMSCINGKAFLCVPEGTSSTLNPTQSDRRLTFHGAAQSQLPD
DCATEDSKCATVTSAVSSKCLLGQPSEKNCKNMEMSETPESHGTPEAPFVGPWDLNTGATHQRESPESDT
GSATTSSDDIKPRSEDYDAGGSQDDEGSHDRGISKCSTALCHDFLGRSSSDTSTPEELKVHEGDLRTEVR
VRKQGGGDHQIHSASDDEIPRKKPEPWSRSTIGYPREKGSTPRGSIPSAQEGDQVSSSADETEDERSEAE
NVGENSSPCSSGTQQVQGIINLAFDDGAEHESREFSATKKFRRSVLLSVDECEEVGSDEGEGHTPFQPSL
DSLSPSDVFDGVSYEHHGRTGYSRFFKENEATTAEHNHNKGNGGYKNESFLLNSRSKDFEKQDKQCVSTD
QKTRLDVPPKGSQQLFPENEEVISKREAAHSFQQHSTFMDGDTKSQERPCHLELHQRELSSNIPKISSVK
SLDSCPSQGLPQEGQVKESRPTPPKGANNAVFSGNVQK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001007574
ORF Size 3264 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001007574.2, NP_001007575.2
RefSeq Size 6756 bp
RefSeq ORF 3267 bp
Locus ID 231570
UniProt ID Q80TK0
Cytogenetics 5 E5
Summary Involved in clathrin-mediated endocytosis at the synapse. Plays a role in neuronal development and in synaptic vesicle recycling in mature neurons, a process required for normal synaptic transmission.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001007574" in other vectors (4)
SKU Description Size Price
MC223523 A830010M20Rik (untagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1, (10ug) 10 ug
$1,043.00
MR215681 A830010M20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$589.00 $947.00
MR215681L3 Lenti ORF clone of A830010M20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$1,247.00
MR215681L4 Lenti ORF clone of A830010M20Rik (mGFP-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$1,247.00

file-alt Citations

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