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A830010M20Rik (NM_001007574) Mouse Untagged Clone

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SKU
MC223523
A830010M20Rik (untagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,043.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol A830010M20Rik
Synonyms AI849226; Kiaa1107; mKIAA1107
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223523 representing NM_001007574
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTTAGATGATCAGCAGAAAATTCAAGTAGCTGCACTTGACAAAGGTGATGACCGCAGACTTGGCA
GGAAGCCTGTGCTCACCAGCTCTCAGCAAAGGAGACAAGGCTCTGATGTTGATGTTTTAAAAATAAAACC
TTGGACAGAAAATAACAAAAAACCGTGTCAGACCTCTCTCTCTACTAATCAGAAGATGAGATCCGATGGA
TTGGGAGCATCAGGACATGCATCAAGCACTAACAGAAACAGTATAAATAAAGTGTCCAAGCATGGTGACA
GTACAAAAATGTCCAAAGTTGTGAAAGAGATGAAAACTGGGGGAAAATATGTCTCTGGCAAGTCTAAAAC
TATGGTAAAGCCCCAAACAGAAAACAGTGATCATACAAAGATAGAAGGTTTGTCATCCACTGTTGTGGGA
AGACCATCAAGAGTGACAGCAGCTGGACGGAAGGACCCAGTACATGGAAAAGGAGTCCAGAACCAAGAAG
TGGAGACCACAGGGGCAAGACCTAAGGTACTCACTGCAAACCTGAACGTGCAAGCCAGAGCCAAGCCTTT
GCAAACACTGAGAGGAAAAGATTCCACATGCCCCGCCTCTGTAGGGCCCTCCAGTAGATCTACACATTCC
AGCACGGAGCTTCTGGCTTCCATGGGCTCTGTGGATGAGACAAAGGAAAACGGATCAGTAGAAGACAAGT
GTCGTGATGGGAAGCCATACGTTAGTGATTCCCCAGGACAGATGGTGAGCAATGGAGTAATGTCAACTGC
AGCAGTAAAGTCTCGAGCAGTTGCAAGAATTACCAATGGAACAGCCAGTAACAAAAGCTTTACTCATGAA
CAAGACTCTCACGGGAATAGCAGTGTAATAAAAAGAGGGGATGGCAAAGGACTTAGTGCTTCAGCACCAC
AGACAGCTGCGAAGAAAAGAGGGAGCAGCAATGGATGCACAGCAGCTCAGCCACGGACGAAGAGCGCCCC
TCCCACCCTGGCTCAAGCTCAAGGATCCCAAGGGGAGTCACCACACTCTGTAAAATCTTCAGTCTCTTCA
AGGCAGTCTGATGAAAATGTAACAAGGTTGAGGCATGGAACAGATAAGCAAATACCTAAGAGAAAAGTTG
TCAAGCAAGGACACACAACTTTGCAGAAGGTTAATGCAAAGCTGGTACCAATGCCTAAAATCCCAAGTCA
GCCCAAGAAAGGTGGAACTGTAAACAGCAAAGATTCAAAACAAAAAGTGCTTCCTGGACAAATTATGCTG
CAAAGTCATGCTTCTCAGCGACCTTCCAAACCTGAGGTAGCTGAGAAAAGCGTGCTTCATGGTGTGAGTG
ACAGCAGGAACCATGTCTCTAAACAGAGGCCTCACGAGTCCCCGTGTACCCTTGCTTGTGACACCAGTGG
TCCTGAAGTGTCCCAGATACCTTGCAGACCTCAGCCACAAAGTCCATTAGGCAGTCAAGAGAAAAAGAGA
CTGGAGCTAGCATGTCAAGATAGGTCAACCCTGGGCGACTCAGTGAAACGTGAGCTGAGGTCAGAACTGG
CTGGCATAGAGCAAAGTCACACATCCGCTTACAAAGACTCGAGTCAGTGCAATGGGAATCCAAACTGTGG
GAGCATTGCTGCTCTGAAATCTGTGATTTCAAATCCAAATGAAAACTTGATAAACTCTAACCCAGTTCAT
AATTCAGACTCAGCAAATACAGAGCAAGCCTATTTATCAGATAGAGAGAGAGAAACAGGGAGAGAAAACA
CAGATACTGAACCAAGTATGAGTTGTATAAATGGGAAAGCTTTCCTGTGTGTTCCTGAAGGGACAAGCAG
TACCCTCAACCCTACTCAAAGTGACAGAAGACTCACATTTCATGGAGCAGCTCAAAGCCAGTTACCTGAC
GACTGTGCTACAGAGGACAGTAAATGTGCTACAGTCACCTCAGCTGTTTCTTCCAAATGTCTTTTGGGAC
AACCATCAGAAAAAAATTGTAAAAATATGGAAATGTCAGAAACCCCAGAGAGCCATGGAACTCCAGAAGC
TCCATTCGTGGGCCCCTGGGATTTGAACACTGGTGCCACACATCAAAGAGAGAGTCCAGAATCCGACACT
GGTAGTGCTACCACATCTTCAGACGACATAAAGCCCCGCTCAGAAGACTATGATGCTGGGGGGTCTCAGG
ATGATGAGGGCTCACACGACAGAGGCATCTCCAAGTGCAGCACAGCGCTGTGCCATGACTTCCTTGGGAG
GAGCAGCAGCGACACCAGCACCCCTGAAGAGTTGAAGGTGCACGAGGGTGACCTCAGGACTGAAGTCAGA
GTGAGAAAGCAGGGTGGGGGTGACCACCAGATCCACTCGGCAAGTGATGACGAGATCCCTAGGAAGAAGC
CAGAACCTTGGTCTCGATCTACAATAGGCTATCCCCGGGAGAAGGGAAGTACGCCACGGGGTAGCATCCC
ATCTGCTCAGGAAGGGGACCAGGTTTCTTCTTCAGCAGATGAAACAGAAGATGAAAGATCTGAAGCAGAG
AATGTTGGGGAAAATTCCTCTCCATGCAGCTCAGGTACTCAGCAGGTTCAGGGAATAATTAACTTAGCTT
TTGACGATGGAGCTGAACATGAAAGTCGTGAGTTTTCTGCAACTAAGAAGTTTAGAAGGTCAGTGTTGCT
CTCGGTAGATGAGTGTGAAGAAGTGGGATCTGATGAAGGTGAAGGCCACACCCCCTTTCAGCCTTCCCTA
GATTCTCTTTCACCTTCTGATGTTTTTGATGGTGTTTCTTATGAGCATCATGGAAGGACAGGTTACTCTA
GATTTTTCAAAGAAAATGAAGCTACTACTGCAGAGCATAATCACAACAAAGGCAATGGTGGATATAAAAA
TGAAAGCTTTCTCCTGAATTCTAGAAGCAAAGACTTCGAAAAACAAGATAAACAATGTGTCTCAACAGAT
CAAAAGACCAGGTTAGATGTCCCACCCAAAGGAAGCCAACAGCTTTTTCCGGAAAACGAAGAAGTGATAA
GTAAACGGGAGGCGGCTCACAGCTTTCAGCAGCACAGCACATTCATGGATGGGGACACTAAGTCTCAAGA
ACGACCGTGTCATTTGGAGCTCCATCAGAGAGAACTCAGCTCCAATATACCAAAGATCAGCTCAGTGAAA
TCTCTGGACTCCTGTCCCAGCCAGGGGCTGCCTCAGGAAGGGCAAGTGAAAGAGAGCCGTCCCACGCCTC
CCAAGGGAGCTAATAACGCTGTGTTCTCAGGTAATGTACAGAAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001007574
Insert Size 3267 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001007574.2, NP_001007575.2
RefSeq Size 6756 bp
RefSeq ORF 3267 bp
Locus ID 231570
UniProt ID Q80TK0
Cytogenetics 5 E5
Summary Involved in clathrin-mediated endocytosis at the synapse. Plays a role in neuronal development and in synaptic vesicle recycling in mature neurons, a process required for normal synaptic transmission.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001007574" in other vectors (4)
SKU Description Size Price
MG215681 A830010M20Rik (tGFP-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik) transcript variant 1, (10ug) 10 ug
$1,147.00
MR215681 A830010M20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$589.00 $947.00
MR215681L3 Lenti ORF clone of A830010M20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$1,247.00
MR215681L4 Lenti ORF clone of A830010M20Rik (mGFP-tagged) - Mouse RIKEN cDNA A830010M20 gene (A830010M20Rik), transcript variant 1 10 ug
$1,247.00

file-alt Citations

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