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Nox3 (NM_198958) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215484
Nox3 (tGFP-tagged) - Mouse NADPH oxidase 3 (Nox3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $729.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nox3
Synonyms GP91-3; het; nmf25; nmf250
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215484 representing NM_198958
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACGCACAGGCTCAAATGGACGGAAAGGAATAACGTGTTGGAGTAAGAGAAGTGTCATGCCGGTGT
GCTGGATTCTGAACGAGAGTGGGTCCTTCGTGGTTGCTCTCTTATGGCTGGCAGTAAACGCCTATCTGTT
TATTGACACATTCTTCTGGTATACTGAAGAGGAGGCTTTCTTTTATACACGAGTTATTCTGGGTTCCGCA
TTGGCATGGGCCCGGGCATCTGCCGTGTGCCTGAATTTTAACTGCATGCTAATTCTGTTACCTGTCAGTC
GGAACTTCATTTCACTGGTGAGAGGAACAAGTGTGTGCTGTAGAGGACCATGGAGAAGACAACTAGACAA
AAACCTCAACTTCCACAAACTCGTTGCCTACGGGATAGCTGTCAATTCAGTTATCCACATTGTGGCACAC
TTGTTCAACCTGGAGCGTTATCACCTGGGTCAGGCCAAGGATGCTGAAGGGCTGCTGGCTGCACTTTCCA
AACTTGGCGATGCCCCAAATGAGAGCTACCTCAATCCAGTCCGCACCTTTGATATGGGCACAACCACTGA
GCTATTGATGACAGTGTCAGGAATTACTGGCCTGGGTATCTCTCTGGCTCTGGTCTTCATCATGACCTCT
TCAACCGAATTCATCAGAAGGTCCTCTTATGAGCTCTTCTGGTACACACACCATATCTTTGTCTTCTTCT
TCATCAGTCTGGCCATCCACGGAGGAGGTCGCATCATTCGAGGCCAAACTCCAGAGAGTCTCCGGCTGCA
CAATGTCACGTACTGCAGAGACCACTATGCTGAATGGCAGGCAGCTGCCTTATGCCCTGTACCTCAATTT
TCTGGCAAGGAACCTTCGGCCTGGAAATGGGCTTTGGGTCCTGTGGTCTTGTATGCGTGTGAAAGAATAA
TTAGGTTCTGGAGATCTCACCAAGAAGTTGTCATTACCAAGGTGGTGAGTCACCCATCTGCAGTCCTGGA
ACTTCACATGAAGAAGCGAGACTTCAAGATGGCACCTGGACAGTACATCTTCATCCAGTGCCCATCTGTC
TCCCCCCTGGAGTGGCACCCCTTCACTCTCACCTCCGCTCCCCAGGAGGACTTCTTCAGTGTACACATCA
GAGCCTCAGGAGACTGGACAGAGGCGTTATTGAAGGCCTTTAGAGTAGAGGGACAGGCTCCCAGTGAGCT
CTGTAGCATGCCGAGGCTAGCAGTGGATGGGCCCTTTGGAGGCTCTCTGGCAGATGTATTTCACTACCCC
GTGAGCGTGTGCATTGCAACGGGAATTGGAGTCACTCCCTTCGCCTCTCTTCTGAAGTCTGTGTGGTATA
AGTGTTGTGAATCACAGAGCCTGCCTGAGCTGAGCAAGGTGTACTTCTATTGGATCTGCCGGGATGCCGG
AGCATTTGAGTGGTTTGCTGATCTGTTACTGTCACTGGAAACACGGATGAGTGAACAAGGGAAGGCTCAT
TTACTGAGCTACCATATATATCTCACTGGCTGGGATGAAAACCAGGCAATTCACATAGCTTTACACTGGG
ATGAAAGTCTGGATGTGATAACAGGCTTAAAGCAGAAGGCTTTCTATGGGCGACCCAACTGGAACGACGA
ATTCAAGCAGATTGCCTACAATCACCCCAGCAGCAGCATTGGCGTGTTCTTCTGTGGATCCAAAGCCATG
TCAAAGACTCTTCAAAAGATGTGTCGTTTGTACTCATCTGTGGATCCGAGGGGCGTTCATTTCTATTACA
ACAAGGAAAACTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215484 representing NM_198958
Red=Cloning site Green=Tags(s)

MQRTGSNGRKGITCWSKRSVMPVCWILNESGSFVVALLWLAVNAYLFIDTFFWYTEEEAFFYTRVILGSA
LAWARASAVCLNFNCMLILLPVSRNFISLVRGTSVCCRGPWRRQLDKNLNFHKLVAYGIAVNSVIHIVAH
LFNLERYHLGQAKDAEGLLAALSKLGDAPNESYLNPVRTFDMGTTTELLMTVSGITGLGISLALVFIMTS
STEFIRRSSYELFWYTHHIFVFFFISLAIHGGGRIIRGQTPESLRLHNVTYCRDHYAEWQAAALCPVPQF
SGKEPSAWKWALGPVVLYACERIIRFWRSHQEVVITKVVSHPSAVLELHMKKRDFKMAPGQYIFIQCPSV
SPLEWHPFTLTSAPQEDFFSVHIRASGDWTEALLKAFRVEGQAPSELCSMPRLAVDGPFGGSLADVFHYP
VSVCIATGIGVTPFASLLKSVWYKCCESQSLPELSKVYFYWICRDAGAFEWFADLLLSLETRMSEQGKAH
LLSYHIYLTGWDENQAIHIALHWDESLDVITGLKQKAFYGRPNWNDEFKQIAYNHPSSSIGVFFCGSKAM
SKTLQKMCRLYSSVDPRGVHFYYNKENF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198958
ORF Size 1764 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198958.2, NP_945196.2
RefSeq Size 1792 bp
RefSeq ORF 1707 bp
Locus ID 224480
UniProt ID Q672J9
Cytogenetics 17 2.05 cM
Summary This gene encodes a member of the NOX family of NADPH oxidases. These enzymes catalyze the transfer of electrons from NADPH to molecular oxygen to produce superoxide and other reactive oxygen species (ROS). The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia, which are crystalline structures of the inner ear involved in the perception of gravity and linear acceleration. In mouse mutations of this gene lead to the absence of otoconia and vestibular dysfunction. provided by RefSeq, Jun 2013
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Documents
Resources
"NM_198958" in other vectors (4)
SKU Description Size Price
MC219460 Nox3 (untagged) - Mouse NADPH oxidase 3 (Nox3), (10ug) 10 ug
$583.00
MR215484 Nox3 (Myc-DDK-tagged) - Mouse NADPH oxidase 3 (Nox3) 10 ug
$289.00 $529.00
MR215484L3 Lenti ORF clone of Nox3 (Myc-DDK-tagged) - Mouse NADPH oxidase 3 (Nox3) 10 ug
$829.00
MR215484L4 Lenti ORF clone of Nox3 (mGFP-tagged) - Mouse NADPH oxidase 3 (Nox3) 10 ug
$829.00

file-alt Citations

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