Nox3 (NM_198958) Mouse Untagged Clone

SKU MC219460

Nox3 (untagged) - Mouse NADPH oxidase 3 (Nox3), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Nox3
• Synonyms: GP91-3; het; nmf25; nmf250
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219460 representing NM_198958; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCGGTGTGCTGGATTCTGAACGAGAGTGGGTCCTTCGTGGTTGCTCTCTTATGGCTGGCAGTAAACG; CCTATCTGTTTATTGACACATTCTTCTGGTATACTGAAGAGGAGGCTTTCTTTTATACACGAGTTATTCT; GGGTTCCGCATTGGCATGGGCCCGGGCATCTGCCGTGTGCCTGAATTTTAACTGCATGCTAATTCTGTTA; CCTGTCAGTCGGAACTTCATTTCACTGGTGAGAGGAACAAGTGTGTGCTGTAGAGGACCATGGAGAAGAC; AACTAGACAAAAACCTCAACTTCCACAAACTCGTTGCCTACGGGATAGCTGTCAATTCAGTTATCCACAT; TGTGGCACACTTGTTCAACCTGGAGCGTTATCACCTGGGTCAGGCCAAGGATGCTGAAGGGCTGCTGGCT; GCACTTTCCAAACTTGGCGATGCCCCAAATGAGAGCTACCTCAATCCAGTCCGCACCTTTGATATGGGCA; CAACCACTGAGCTATTGATGACAGTGTCAGGAATTACTGGCCTGGGTATCTCTCTGGCTCTGGTCTTCAT; CATGACCTCTTCAACCGAATTCATCAGAAGGTCCTCTTATGAGCTCTTCTGGTACACACACCATATCTTT; GTCTTCTTCTTCATCAGTCTGGCCATCCACGGAGGAGGTCGCATCATTCGAGGCCAAACTCCAGAGAGTC; TCCGGCTGCACAATGTCACGTACTGCAGAGACCACTATGCTGAATGGCAGGCAGCTGCCTTATGCCCTGT; ACCTCAATTTTCTGGCAAGGAACCTTCGGCCTGGAAATGGGCTTTGGGTCCTGTGGTCTTGTATGCGTGT; GAAAGAATAATTAGGTTCTGGAGATCTCACCAAGAAGTTGTCATTACCAAGGTGGTGAGTCACCCATCTG; CAGTCCTGGAACTTCACATGAAGAAGCGAGACTTCAAGATGGCACCTGGACAGTACATCTTCATCCAGTG; CCCATCTGTCTCCCCCCTGGAGTGGCACCCCTTCACTCTCACCTCCGCTCCCCAGGAGGACTTCTTCAGT; GTACACATCAGAGCCTCAGGAGACTGGACAGAGGCGTTATTGAAGGCCTTTAGAGTAGAGGGACAGGCTC; CCAGTGAGCTCTGTAGCATGCCGAGGCTAGCAGTGGATGGGCCCTTTGGAGGCTCTCTGGCAGATGTATT; TCACTACCCCGTGAGCGTGTGCATTGCAACGGGAATTGGAGTCACTCCCTTCGCCTCTCTTCTGAAGTCT; GTGTGGTATAAGTGTTGTGAATCACAGAGCCTGCCTGAGCTGAGCAAGGTGTACTTCTATTGGATCTGCC; GGGATGCCGGAGCATTTGAGTGGTTTGCTGATCTGTTACTGTCACTGGAAACACGGATGAGTGAACAAGG; GAAGGCTCATTTACTGAGCTACCATATATATCTCACTGGCTGGGATGAAAACCAGGCAATTCACATAGCT; TTACACTGGGATGAAAGTCTGGATGTGATAACAGGCTTAAAGCAGAAGGCTTTCTATGGGCGACCCAACT; GGAACGACGAATTCAAGCAGATTGCCTACAATCACCCCAGCAGCAGCATTGGCGTGTTCTTCTGTGGATC; CAAAGCCATGTCAAAGACTCTTCAAAAGATGTGTCGTTTGTACTCATCTGTGGATCCGAGGGGCGTTCAT; TTCTATTACAACAAGGAAAACTTCTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198958
• Insert Size: 1707 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198958.2, NP_945196.2
• RefSeq Size: 1792 bp
• RefSeq ORF: 1707 bp
• Locus ID: 224480
• UniProt ID: Q672J9
• Cytogenetics: 17 2.05 cM
• Summary: This gene encodes a member of the NOX family of NADPH oxidases. These enzymes catalyze the transfer of electrons from NADPH to molecular oxygen to produce superoxide and other reactive oxygen species (ROS). The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia, which are crystalline structures of the inner ear involved in the perception of gravity and linear acceleration. In mouse mutations of this gene lead to the absence of otoconia and vestibular dysfunction. [provided by RefSeq, Jun 2013]