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Magea4 (NM_020280) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215418
Magea4 (tGFP-tagged) - Mouse melanoma antigen family A 4 (Magea4), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Magea4 or NM_020280"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Magea4
Synonyms Mage-a4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215418 representing NM_020280
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAATCCCGATAACACCCATTACTGCAGCCTCCAAGGCAGTGCTCGGAGCCAAAGGGAGTTAGACA
ATGTTCAGGCTACAATAGCTGTGGTAGATGAAGAGGAAGCCACTCCCACCTCAAAGAAGGTGTACAGTAG
TGGAATACCAAGTCCTCCCCAGAGTCCTCAAAGAGCCTCCTCTCCCCTTGTGATACTAGCCTCTGTTCCT
GAAGGCCCATCTGAGGAAGCTTCCACCAACCAAGTAGAGGAACAGGAAGACCCACTGTGCATGTTGTACA
ATGCACAGAACATAAAGTTATATGAATTGGCAACCTTTCTGTTTTTCAAATATCAAATGAAGGCATTTAC
TACCAAAGCAGAAATGCTGGAAAGTATTGGTAGAGAGTATGAGGAGTACTACCCTCTGATCTTTAGTGAG
GCCTCTGAGTGCTTTAAGCTGGTCTCTGGCATTGGCATGATAGAAGTGGACCCATTTGTCCAATCCTATA
TCCTTGTCCCTGCCCTGGGGCTCACCTATGATGGGATGCTGACTGATGTTCAGAGTAAGCCCAAGACAGG
TATCCTAATAGTTGTTCTTGGTGCTATTTTCACGCAAGGAAACTGTGTCAGTGAGGAGATTATCTGGAAA
ATGCTGAATAACATAGGGTTGTATGGTGGGAGGCATCTTTACATACATCAAGACCCCAGGAAGTTCATCT
CTGAGGAGTTTGTGCAGGAAGGGTACCTGGAATACAGGCAGGTGCCTAATAGTGATCCTCCAAGCTATGA
GTTTCTGTGGGACCCAAGGGCCTTTGCAGAAACCACCAAAATGAAAGTCTTGGAGATTTTTGCCAGCATT
ACTATGACCGATCCCAGAGCTTACACTGAAAAATACGCAGAGGCTTTGAGAGATGAGACAGAGAGGGCCC
AGGCCAGGATCATCCAGCAGATAGTTCTACAGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215418 representing NM_020280
Red=Cloning site Green=Tags(s)

MENPDNTHYCSLQGSARSQRELDNVQATIAVVDEEEATPTSKKVYSSGIPSPPQSPQRASSPLVILASVP
EGPSEEASTNQVEEQEDPLCMLYNAQNIKLYELATFLFFKYQMKAFTTKAEMLESIGREYEEYYPLIFSE
ASECFKLVSGIGMIEVDPFVQSYILVPALGLTYDGMLTDVQSKPKTGILIVVLGAIFTQGNCVSEEIIWK
MLNNIGLYGGRHLYIHQDPRKFISEEFVQEGYLEYRQVPNSDPPSYEFLWDPRAFAETTKMKVLEIFASI
TMTDPRAYTEKYAEALRDETERAQARIIQQIVLQL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020280
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020280.2, NP_064676.2
RefSeq Size 948 bp
RefSeq ORF 948 bp
Locus ID 17140
Cytogenetics X 37.2 cM
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Documents
Resources
"NM_020280" in other vectors (4)
SKU Description Size Price
MC208930 Magea4 (untagged) - Mouse melanoma antigen, family A, 4 (Magea4), (10ug) 10 ug
$330.00
MR215418 Magea4 (Myc-DDK-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$289.00 $300.00
MR215418L3 Lenti ORF clone of Magea4 (Myc-DDK-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$600.00
MR215418L4 Lenti ORF clone of Magea4 (mGFP-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.