Magea4 (NM_020280) Mouse Untagged Clone

SKU
MC208930
Magea4 (untagged) - Mouse melanoma antigen, family A, 4 (Magea4), (10ug)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Magea4
Synonyms Mage-a4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208930 representing NM_020280
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAATCCCGATAACACCCATTACTGCAGCCTCCAAGGCAGTGCTCGGAGCCAAAGGGAGTTAGACA
ATGTTCAGGCTACAATAGCTGTGGTAGATGAAGAGGAAGCCACTCCCACCTCAAAGAAGGTGTACAGTAG
TGGAATACCAAGTCCTCCCCAGAGTCCTCAAAGAGCCTCCTCTCCCCTTGTGATACTAGCCTCTGTTCCT
GAAGGCCCATCTGAGGAAGCTTCCACCAACCAAGTAGAGGAACAGGAAGACCCACTGTGCATGTTGTACA
ATGCACAGAACATAAAGTTATATGAATTGGCAACCTTTCTGTTTTTCAAATATCAAATGAAGGCATTTAC
TACCAAAGCAGAAATGCTGGAAAGTATTGGTAGAGAGTATGAGGAGTACTACCCTCTGATCTTTAGTGAG
GCCTCTGAGTGCTTTAAGCTGGTCTCTGGCATTGGCATGATAGAAGTGGACCCATTTGTCCAATCCTATA
TCCTTGTCCCTGCCCTGGGGCTCACCTATGATGGGATGCTGACTGATGTTCAGAGTAAGCCCAAGACAGG
TATCCTAATAGTTGTTCTTGGTGCTATTTTCACGCAAGGAAACTGTGTCAGTGAGGAGATTATCTGGAAA
ATGCTGAATAACATAGGGTTGTATGGTGGGAGGCATCTTTACATACATCAAGACCCCAGGAAGTTCATCT
CTGAGGAGTTTGTGCAGGAAGGGTACCTGGAATACAGGCAGGTGCCTAATAGTGATCCTCCAAGCTATGA
GTTTCTGTGGGACCCAAGGGCCTTTGCAGAAACCACCAAAATGAAAGTCTTGGAGATTTTTGCCAGCATT
ACTATGACCGATCCCAGAGCTTACACTGAAAAATACGCAGAGGCTTTGAGAGATGAGACAGAGAGGGCCC
AGGCCAGGATCATCCAGCAGATAGTTCTACAGCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_020280
Insert Size 948 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020280.2, NP_064676.2
RefSeq Size 948 bp
RefSeq ORF 948 bp
Locus ID 17140
Cytogenetics X 37.2 cM
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Documents
Resources
"NM_020280" in other vectors (4)
SKU Description Size Price
MG215418 Magea4 (tGFP-tagged) - Mouse melanoma antigen family A 4 (Magea4), (10ug) 10 ug
$500.00
MR215418 Magea4 (Myc-DDK-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$289.00 $300.00
MR215418L3 Lenti ORF clone of Magea4 (Myc-DDK-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$600.00
MR215418L4 Lenti ORF clone of Magea4 (mGFP-tagged) - Mouse melanoma antigen, family A, 4 (Magea4) 10 ug
$600.00

file-alt Citations

Expression of tumor antigens on primary ovarian cancer cells compared to established ovarian cancer cell lines
Kloudová, Hromádková, Partlová et al
Oncotarget (2016) 7 (29), 46120-46126 DOI: 10.18632/oncotarget.10028
Snippet: Recombinant proteins NY-ESO-1, Her-2/neu, MAGE-A4 (Origene, Rockville, US), CA125 (R&D Systems) and MAGE-A3, MAGE-A10 (Abnova, Taipei, Taiwan) were diluted in Carbonate Coating Buffer (Invitrogen, Prague, Czech Republic) to a final concentration of 1 μg/ml and coated to 96-well plates overnight at 4°C
FigureFigure
Showing 1-1 of 1 papers.
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