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Drc1 (NM_001033460) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214733
Ccdc164 (tGFP-tagged) - Mouse predicted gene 1060 (Gm1060), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $889.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Drc1
Synonyms b2b1654Clo; Ccdc164; Gm1060
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214733 representing NM_001033460
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCCTCAGGAACCATTGGGGTCCTTGAGCAGAATGGGGAAGAACACTTAGCCACCCCGATTCTCG
GACCCTCGGTGCATTCGGATAACCCGCAGGAGCGGATCCAGGCCCGGCGCCTGCGCATCGCCGCTCGTCA
GGAAGCCCGAAGGCGGGAAGCCCTGGGAGAGTATTTGGACGGGAAGAAGGAGAGTGAGGAGGAGCAGAGC
AAGAGCTACAAACAGAAAGAAGAGAGCCGCCTGAAACTGACTAAACTCCTGCTCTGCGGGACCGAGTTGG
TGACAAATATTCAGGTGGCTGCAGACGTCAGAGAGATCCACAGGAGGGTTGAAGAGGAGGAGACAAAGCG
GCAGAGACTGGAGAAGCTGGAGAATGAAGTTAAGACCAGCCAGGATAAATTTGATGAAATCACGGCGAAG
TGGGAGGAGGGCAGACGGAAGCGGATTCCCCAGGAACTGTGGGAGATGCTCAACAGCCAGCAGGTGCACT
GTGCTGAGCTCATAGAAGACAAGAACAAGCTGGCCAACGAGCTGCAGCAGGAGTTAAAAATAAAGGATGA
CCAGTATGTGAAGGACTTGAAGAAACAGTCGGAAGACATCACCCTGATTCTGGAGAGAATGGAGGAGCAG
GTGAAGAATGTGATGAAGAACTTCCGCCAGGAGCTGATTCACATCGAGAAAGCCTTTGAGTCAGAACGGC
AAGAACTGCTATCCAGCAATAAGAAGAAATGGGAGCGAGCCCTGCAGGCTCACAATGCCAAGGAGCTGGA
GTACCTCACCAACCGCATGAAGAAGGTGGAGGACTACGAGAAGCAGCTCAACAAGCAGCGGGTCTGGGAC
TGCGAGGAGTACAACACAATCAAGATCAAGCTAGAGCAGGATGTGCAGATTCTTGAGCAACAGCTTCAGC
AGATGAAAGCAACTTACCAGCTAAACCAAGAGAAGCTGGAGTACAACTTTCAGGTCCTGAAGAAGAGAGA
TGAAGAGAGCACAGTGATCAAGTCCCAGCAGAAGAGGAAACTGAACCGCCTGCACGATGTCGTCAACAAT
CTGAGAACAAAATACACCAAGCAGATACGGCAGTTTCAGGATGACAACCAGTCCCTGACCTCCGACTACA
AACGGCTTGTGACGCAATTCAAGGACCTACAGAAAGCCTTGAGACACTTCATCATCATCGATGAGGAGAA
GTTTCGGGAGATTTGGCTGATGAATGAAGCAGAGGCGAAGGAACTGGCACAAAGAGCCTTTGATGTGGAC
AGGATCATCCATTCCCAGCACCTGGGCTTACCCTGGAATATGCCTGACTTGTGGTTCCTGAATAACGTGG
GGCCGATTTCCCTGCAGCAGCAGAAGTCAGTCACACAAATACTGGAGGAACTGCTGTTGCAGACAGAGGA
TGAGGCCACAGAGGCAGCCATGTCGGAAGACGAGGATTACATGGACCTGCCCAATCAAATTTCAGCCAAA
ACCACCACAAAGGTCCTGATGCTCCTGTGTGATGAGTCGGGTTTCCTCATAGAGAGCAAACTACTGAGTC
TGCTCCACCCCCTGGAGAAGAGTGAATGCTATCTGCTGAGGTTGGACGCCATCTTCTCCGCCCTGGCAAT
CGAAGACGAGGATGACTTATACAAGCTGGTAAACTTCTTCCTTCGATACCGAGCCCACCGTCTATCCTCT
GCCCAGGCCAGTTCCTCCATTCATAGCAATATGGAGCGTACAAGCCTGATGTCAGCACTAGAGCGTTTGA
GTCTGATGTCCCAGACAGACAAGGGAAGCATGGTGTCCAAATCAGATCAGGAGCCCACAGAGCAGGAGGA
CGAGCAGGAAGGTGACAACGCAAGCCTCTCGAGCAGGGAGTTGGAAGAGCAGGAGGACCTGTCATCACCC
AGGTTCATCCACCCCAATGATGTTCTCAAGATTCTGGAGGCCTTTGTCACGGGGCTGAAGAAGCCTAAGG
ATGCTCAGCCAGTACTGAAATTGAAGAAAGAGACTCGAGATAACTCGAAGGACACGGAGTACTGGGAATC
CCTGGCTGCGGTTATTCCTTTCTTCAAGCAGAACCTCTGGGATGCGCTGTACAAGGCCTTGGAGAAATAC
TATCTCGTGCTGACCGAGAGGGCCAAGCTGCTGATGGAGAATGAGTCTCTGGAGCAGCAGAATGCAGAGA
TGCAGTCCCTGCTGCAGCAGTATCTCCAGGCCAAGGTAAACACAGAACTGCAGATCCCACCAACTCAGGG
CTTCCGGATGCCCTCAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214733 representing NM_001033460
Red=Cloning site Green=Tags(s)

MNPSGTIGVLEQNGEEHLATPILGPSVHSDNPQERIQARRLRIAARQEARRREALGEYLDGKKESEEEQS
KSYKQKEESRLKLTKLLLCGTELVTNIQVAADVREIHRRVEEEETKRQRLEKLENEVKTSQDKFDEITAK
WEEGRRKRIPQELWEMLNSQQVHCAELIEDKNKLANELQQELKIKDDQYVKDLKKQSEDITLILERMEEQ
VKNVMKNFRQELIHIEKAFESERQELLSSNKKKWERALQAHNAKELEYLTNRMKKVEDYEKQLNKQRVWD
CEEYNTIKIKLEQDVQILEQQLQQMKATYQLNQEKLEYNFQVLKKRDEESTVIKSQQKRKLNRLHDVVNN
LRTKYTKQIRQFQDDNQSLTSDYKRLVTQFKDLQKALRHFIIIDEEKFREIWLMNEAEAKELAQRAFDVD
RIIHSQHLGLPWNMPDLWFLNNVGPISLQQQKSVTQILEELLLQTEDEATEAAMSEDEDYMDLPNQISAK
TTTKVLMLLCDESGFLIESKLLSLLHPLEKSECYLLRLDAIFSALAIEDEDDLYKLVNFFLRYRAHRLSS
AQASSSIHSNMERTSLMSALERLSLMSQTDKGSMVSKSDQEPTEQEDEQEGDNASLSSRELEEQEDLSSP
RFIHPNDVLKILEAFVTGLKKPKDAQPVLKLKKETRDNSKDTEYWESLAAVIPFFKQNLWDALYKALEKY
YLVLTERAKLLMENESLEQQNAEMQSLLQQYLQAKVNTELQIPPTQGFRMPSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033460
ORF Size 2259 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033460.3, NP_001028632.1
RefSeq Size 2485 bp
RefSeq ORF 2262 bp
Locus ID 381738
UniProt ID Q3USS3
Cytogenetics 5 B1
Summary Component of the nexin-dynein regulatory complex (N-DRC) a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with CCDC65/DRC2 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033460" in other vectors (4)
SKU Description Size Price
MC221349 Drc1 (untagged) - Mouse coiled-coil domain containing 164 (Ccdc164), (10ug) 10 ug
$759.00
MR214733 Ccdc164 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 164 (Ccdc164) 10 ug
$589.00 $689.00
MR214733L3 Lenti ORF clone of Ccdc164 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 164 (Ccdc164) 10 ug
$989.00
MR214733L4 Lenti ORF clone of Ccdc164 (mGFP-tagged) - Mouse coiled-coil domain containing 164 (Ccdc164) 10 ug
$989.00

file-alt Citations

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