Top3b (NM_011624) Mouse Tagged ORF Clone

SKU MG210989

Top3b (tGFP-tagged) - Mouse topoisomerase (DNA) III beta (Top3b)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Top3b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210989 representing NM_011624; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGACCGTGCTCATGGTAGCAGAAAAGCCATCCCTGGCACAGTCCATTGCCAAAATCCTCTCCCGAG; GAAACATGTCCTCACACAAAGGGCTGAATGGGGCATGTTCAGTGCACAAGTACACAGGAACCTTTGCTGG; CCAACCTGTCCACTTCAAGATGACGTCTGTCTGCGGTCATGTGATGACACTGGATTTTCTGGGGAAGTAC; AACAAGTGGGATAAGGTGGATCCTGCAGAGCTCTTCAGCCAAGCTCCAACAGAGAAAAAGGAAGCTAACC; CCAAGCTGAACATGGTCAAGTTCCTGCAGGTGGAGGGCAGAGGCTGTGACTATGTTGTGCTGTGGCTGGA; CTGTGACAAGGAAGGGGAGAATATTTGCTTTGAGGTCCTTGATGCCGTTCTGCCTGTCATGAATAATGCC; CACAATGGTGAGAAGACAGTGTTTCGTGCCAGGTTCAGCTCCATCACTGATACAGACATCTGTAATGCCA; TGACCCGCCTGAGCGAGCCAGACCACAATGAGGCACTCTCAGTGGATGCCCGCCAGGAGCTAGACCTGCG; CATCGGCTGTGCATTCACTAGGTTTCAAACAAAGTATTTCCAGGGAAAATATGGCGATTTAGACAGCTCT; CTCATCTCCTTTGGGCCATGTCAGACTCCTACCCTGGGATTCTGTGTAGAAAGACATGATAAAATCCAGT; CCTTCAAGCCAGAGACCTACTGGGTGCTTCAGGCCAAGGTTCATACAGATAAAGAGGAGTCACTCCTTCT; GGACTGGGACCGTGTTAGAGTTTTCGACTGGGAGATTGCACAGATGTTTTTAAACATGACAAAGTTAGAA; AAAGAAGCTTGGGTGGAGGCTACAAGCAGGAAAGAAAAAGCCAAGCAGAGACCACTTGCTCTGAACACTG; TGGAGATGTTGCGTGTGGCCAGCTCAGCTCTGGGTATGGGACCACAGCACGCCATGCAAATAGCTGAGAG; ATTATACACACAGGGCTATATCAGCTACCCACGGACAGAAACCACCCACTATCCCGAGAACTTTGACCTG; AAGGGCTCTTTGCGACAGCAGGCCAATCACCCATACTGGGCAGACTCGGTAAAGCAGTTGTTAGCGGAAG; GCATCAACCGCCCTCGGAAAGGTCATGATGCTGGTGACCATCCTCCAATCACACCTATGAAGTCGGCTAC; AGAGGCTGAATTAGGAGGTGACGCATGGAGACTCTACGAGTATATCACCAGACACTTCATTGCCACAGTG; AGCCATGACTGCAAGTATCTCCAGAGCACTATCTCCTTCAGGATCGGGCCTGAACACTTTACATGCATGG; GAAAAACAGTTATTTCTCCAGGCTTCACAGAGATCATGCCTTGGCAGAGCGTGCCCCTGGAAGAGAGTCT; GCCCACGTGCCAGAAGGGTGATACCTTCACTGTGGGTGAAGTGAAGATGCTAGAGAAGCAGACGAGCCCA; CCGGACTATCTGACTGAGGCTGAGCTCATCACTCTCATGGAGAAGCATGGTATCGGGACTGATGCCAGTA; TCCCTGTGCACATCAACAATATCTGCCAGCGCAACTATGTTACTGTGGAGAGTGGCCGCCGACTCAAGCC; CACCAACCTTGGCATAGTTCTGGTGCATGGTTACTACAAGATTGATGCAGAGCTGGTACTCCCCACCATC; CGAAGCGCAGTGGAGAAGCAGCTGAATCTTATTGCCCAGGGCAAAGCTGACTACCACCAAGTTCTGGGCC; ATACCCTGGATATCTTCAAGAGAAAGTTCCACTACTTCGTTGATTCTATTGCTGGCATGGACGAGTTGAT; GGAGGTTTCCTTTTCACCCCTGGCTGCCACTGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCATCGATTT; ATGAAGTACATCCAGGCCAAGCCAAGTCGCCTGCACTGCTCCCACTGTGATGAGACCTACACCCTCCCCC; AGAATGGTACCATCAAGCTCTACAAGGAGCTGCGGTGCCCATTGGATGACTTTGAACTGGTCCTGTGGTC; CTCAGGCTCCCGGGGCAAGAGCTACCCACTATGTCCCTACTGCTATAACCACCCACCCTTCCGAGACATG; AAGAAAGGCATGGGCTGCAATGAGTGCACACACCCCACCTGCCAGCACTCACTGAGTATGCTGGGCATTG; GCCAGTGCGTGGAATGCGAGAATGGAGTCCTTGTTCTGGACCCCACTTCTGGTCCCAAGTGGAAGGTGGC; CTGCAATACATGCAATGTGGTGGCACACTGCTTTGAAAATGCCCACCGAGTACGGGTATCTGCTGACACC; TGCAACACCTGCGAGGCTGCCTTGCTCGATGTAGACTTCAACAAGGCCAAGTCTCCCCTTCCTGGCAATG; AGACACAGCACACAGGCTGCATCTTCTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTTAAGCATGCAGC; CTCCTGCCACCCCATGCACCGAGGTGGGCCTGGAAGGAGGCAGGGTCGTGGGCGGGGCCGGGGCCGGAGA; CCTCCAGGAAAGCCCAACCCTAGGCGGCCAAAAGACAAGATGTCAGCTCTGGCTGCTTACTTTGTA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210989 representing NM_011624; Red=Cloning site Green=Tags(s); ; MKTVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMTLDFLGKY; NKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVLPVMNNA; HNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSS; LISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLE; KEAWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDL; KGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATV; SHDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSP; PDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTI; RSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRF; MKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDM; KKGMGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADT; CNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRGRR; PPGKPNPRRPKDKMSALAAYFV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011624
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_011624.3
• RefSeq Size: 2987 bp
• RefSeq ORF: 2589 bp
• Locus ID: 21976
• UniProt ID: Q9Z321
• Cytogenetics: 16 A3
• Summary: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.[UniProtKB/Swiss-Prot Function]