OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Top3b (NM_011624) Mouse Untagged Clone

Be the first to review this product
SKU
MC201726
Top3b (untagged) - Mouse topoisomerase (DNA) III beta (Top3b), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $790.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Top3b or NM_011624"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Top3b
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC031723 sequence for NM_011624
CCGGAATGGTAGCAGCGCTCCGACCCGGGCGGGGGCGTGGGGGAGGGTCCTGCCCTGGCGCGGCTTTTTC GCCATCCAGCCCTCGCCGTGGGGCCTCTGGGCGGCGGTGGAGCCGCGTTTAGTGGGCAGTGGTCGCTTTT CCAACGAGCTCCCCGCGGCCCCGAGGAGTCAGCATTGGCAGTCGTGTTGAGCTACAGAGGAGACATTTCT AAGCTTTTTTATTGCTGCTGTTCTGGGTCTGGGTGCAACGGTCTGTGGGCCCTGACAGCCAGGAGCAAGA ATGAAGACCGTGCTCATGGTAGCAGAAAAGCCATCCCTGGCACAGTCCATTGCCAAAATCCTCTCCCGAG GAAACATGTCCTCACACAAAGGGCTGAATGGGGCATGTTCAGTGCACAAGTACACAGGAACCTTTGCTGG CCAACCTGTCCACTTCAAGATGACGTCTGTCTGCGGTCATGTGATGACACTGGATTTTCTGGGGAAGTAC AACAAGTGGGATAAGGTGGATCCTGCAGAGCTCTTCAGCCAAGCTCCAACAGAGAAAAAGGAAGCTAACC CCAAGCTGAACATGGTCAAGTTCCTGCAGGTGGAGGGCAGAGGCTGTGACTATGTTGTGCTGTGGCTGGA CTGTGACAAGGAAGGGGAGAATATTTGCTTTGAGGTCCTTGATGCCGTTCTGCCTGTCATGAATAATGCC CACAATGGTGAGAAGACAGTGTTTCGTGCCAGGTTCAGCTCCATCACTGATACAGACATCTGTAATGCCA TGACCCGCCTGAGCGAGCCAGACCACAATGAGGCACTCTCAGTGGATGCCCGCCAGGAGCTAGACCTGCG CATCGGCTGTGCATTCACTAGGTTTCAAACAAAGTATTTCCAGGGAAAATATGGCGATTTAGACAGCTCT CTCATCTCCTTTGGGCCATGTCAGACTCCTACCCTGGGATTCTGTGTAGAAAGACATGATAAAATCCAGT CCTTCAAGCCAGAGACCTACTGGGTGCTTCAGGCCAAGGTTCATACAGATAAAGAGGAGTCACTCCTTCT GGACTGGGACCGTGTTAGAGTTTTCGACTGGGAGATTGCACAGATGTTTTTAAACATGACAAAGTTAGAA AAAGAAGCTTGGGTGGAGGCTACAAGCAGGAAAGAAAAAGCCAAGCAGAGACCACTTGCTCTGAACACTG TGGAGATGTTGCGTGTGGCCAGCTCAGCTCTGGGTATGGGACCACAGCACGCCATGCAAATAGCTGAGAG ATTATACACACAGGGCTATATCAGCTACCCACGGACAGAAACCACCCACTATCCCGAGAACTTTGACCTG AAGGGCTCTTTGCGACAGCAGGCCAATCACCCATACTGGGCAGACTCGGTAAAGCAGTTGTTAGCGGAAG GCATCAACCGCCCTCGGAAAGGTCATGATGCTGGTGACCATCCTCCAATCACACCTATGAAGTCGGCTAC AGAGGCTGAATTAGGAGGTGACGCATGGAGACTCTACGAGTATATCACCAGACACTTCATTGCCACAGTG AGCCATGACTGCAAGTATCTCCAGAGCACTATCTCCTTCAGGATCGGGCCTGAACACTTTACATGCATGG GAAAAACAGTTATTTCTCCAGGCTTCACAGAGATCATGCCTTGGCAGAGCGTGCCCCTGGAAGAGAGTCT GCCCACGTGCCAGAAGGGTGATACCTTCACTGTGGGTGAAGTGAAGATGCTAGAGAAGCAGACGAGCCCA CCGGACTATCTGACTGAGGCTGAGCTCATCACTCTCATGGAGAAGCATGGTATCGGGACTGATGCCAGTA TCCCTGTGCACATCAACAATATCTGCCAGCGCAACTATGTTACTGTGGAGAGTGGCCGCCGACTCAAGCC CACCAACCTTGGCATAGTTCTGGTGCATGGTTACTACAAGATTGATGCAGAGCTGGTACTCCCCACCATC CGAAGCGCAGTGGAGAAGCAGCTGAATCTTATTGCCCAGGGCAAAGCTGACTACCACCAAGTTCTGGGCC ATACCCTGGATATCTTCAAGAGAAAGTTCCACTACTTCGTTGATTCTATTGCTGGCATGGACGAGTTGAT GGAGGTTTCCTTTTCACCCCTGGCTGCCACTGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCATCGATTT ATGAAGTACATCCAGGCCAAGCCAAGTCGCCTGCACTGCTCCCACTGTGATGAGACCTACACCCTCCCCC AGAATGGTACCATCAAGCTCTACAAGGAGCTGCGGTGCCCATTGGATGACTTTGAACTGGTCCTGTGGTC CTCAGGCTCCCGGGGCAAGAGCTACCCACTATGTCCCTACTGCTATAACCACCCACCCTTCCGAGACATG AAGAAAGGCATGGGCTGCAATGAGTGCACACACCCCACCTGCCAGCACTCACTGAGTATGCTGGGCATTG GCCAGTGCGTGGAATGCGAGAATGGAGTCCTTGTTCTGGACCCCACTTCTGGTCCCAAGTGGAAGGTGGC CTGCAATACATGCAATGTGGTGGCACACTGCTTTGAAAATGCCCACCGAGTACGGGTATCTGCTGACACC TGCAACACCTGCGAGGCTGCCTTGCTCGATGTAGACTTCAACAAGGCCAAGTCTCCCCTTCCTGGCAATG AGACACAGCACACAGGCTGCATCTTCTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTTAAGCATGCAGC CTCCTGCCACCCCATGCACCGAGGTGGGCCTGGAAGGAGGCAGGGTCGTGGGCGGGGCCGGGGCCGGAGA CCTCCAGGAAAGCCCAACCCTAGGCGGCCAAAAGACAAGATGTCAGCTCTGGCTGCTTACTTTGTATGAC AACCTATCTTTTCCCCACTCCAGACACATCGTGGTTATCTTTGAGCCACTGAAACCATTAAAATGCTTTT TATTTTGTATTCTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_011624
Insert Size 2589 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031723, AAH31723
RefSeq Size 2987 bp
RefSeq ORF 2589 bp
Locus ID 21976
UniProt ID Q9Z321
Cytogenetics 16 A3
Summary Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_011624" in other vectors (4)
SKU Description Size Price
MG210989 Top3b (tGFP-tagged) - Mouse topoisomerase (DNA) III beta (Top3b) 10 ug
$990.00
MR210989 Top3b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III beta (Top3b) 10 ug
$589.00 $790.00
MR210989L3 Lenti ORF clone of Top3b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III beta (Top3b) 10 ug
$1,090.00
MR210989L4 Lenti ORF clone of Top3b (mGFP-tagged) - Mouse topoisomerase (DNA) III beta (Top3b) 10 ug
$1,090.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products