Top3b (NM_011624) Mouse Untagged Clone

SKU MC201726

Top3b (untagged) - Mouse topoisomerase (DNA) III beta (Top3b), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Top3b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031723 sequence for NM_011624; CCGGAATGGTAGCAGCGCTCCGACCCGGGCGGGGGCGTGGGGGAGGGTCCTGCCCTGGCGCGGCTTTTTC GCCATCCAGCCCTCGCCGTGGGGCCTCTGGGCGGCGGTGGAGCCGCGTTTAGTGGGCAGTGGTCGCTTTT CCAACGAGCTCCCCGCGGCCCCGAGGAGTCAGCATTGGCAGTCGTGTTGAGCTACAGAGGAGACATTTCT AAGCTTTTTTATTGCTGCTGTTCTGGGTCTGGGTGCAACGGTCTGTGGGCCCTGACAGCCAGGAGCAAGA ATGAAGACCGTGCTCATGGTAGCAGAAAAGCCATCCCTGGCACAGTCCATTGCCAAAATCCTCTCCCGAG GAAACATGTCCTCACACAAAGGGCTGAATGGGGCATGTTCAGTGCACAAGTACACAGGAACCTTTGCTGG CCAACCTGTCCACTTCAAGATGACGTCTGTCTGCGGTCATGTGATGACACTGGATTTTCTGGGGAAGTAC AACAAGTGGGATAAGGTGGATCCTGCAGAGCTCTTCAGCCAAGCTCCAACAGAGAAAAAGGAAGCTAACC CCAAGCTGAACATGGTCAAGTTCCTGCAGGTGGAGGGCAGAGGCTGTGACTATGTTGTGCTGTGGCTGGA CTGTGACAAGGAAGGGGAGAATATTTGCTTTGAGGTCCTTGATGCCGTTCTGCCTGTCATGAATAATGCC CACAATGGTGAGAAGACAGTGTTTCGTGCCAGGTTCAGCTCCATCACTGATACAGACATCTGTAATGCCA TGACCCGCCTGAGCGAGCCAGACCACAATGAGGCACTCTCAGTGGATGCCCGCCAGGAGCTAGACCTGCG CATCGGCTGTGCATTCACTAGGTTTCAAACAAAGTATTTCCAGGGAAAATATGGCGATTTAGACAGCTCT CTCATCTCCTTTGGGCCATGTCAGACTCCTACCCTGGGATTCTGTGTAGAAAGACATGATAAAATCCAGT CCTTCAAGCCAGAGACCTACTGGGTGCTTCAGGCCAAGGTTCATACAGATAAAGAGGAGTCACTCCTTCT GGACTGGGACCGTGTTAGAGTTTTCGACTGGGAGATTGCACAGATGTTTTTAAACATGACAAAGTTAGAA AAAGAAGCTTGGGTGGAGGCTACAAGCAGGAAAGAAAAAGCCAAGCAGAGACCACTTGCTCTGAACACTG TGGAGATGTTGCGTGTGGCCAGCTCAGCTCTGGGTATGGGACCACAGCACGCCATGCAAATAGCTGAGAG ATTATACACACAGGGCTATATCAGCTACCCACGGACAGAAACCACCCACTATCCCGAGAACTTTGACCTG AAGGGCTCTTTGCGACAGCAGGCCAATCACCCATACTGGGCAGACTCGGTAAAGCAGTTGTTAGCGGAAG GCATCAACCGCCCTCGGAAAGGTCATGATGCTGGTGACCATCCTCCAATCACACCTATGAAGTCGGCTAC AGAGGCTGAATTAGGAGGTGACGCATGGAGACTCTACGAGTATATCACCAGACACTTCATTGCCACAGTG AGCCATGACTGCAAGTATCTCCAGAGCACTATCTCCTTCAGGATCGGGCCTGAACACTTTACATGCATGG GAAAAACAGTTATTTCTCCAGGCTTCACAGAGATCATGCCTTGGCAGAGCGTGCCCCTGGAAGAGAGTCT GCCCACGTGCCAGAAGGGTGATACCTTCACTGTGGGTGAAGTGAAGATGCTAGAGAAGCAGACGAGCCCA CCGGACTATCTGACTGAGGCTGAGCTCATCACTCTCATGGAGAAGCATGGTATCGGGACTGATGCCAGTA TCCCTGTGCACATCAACAATATCTGCCAGCGCAACTATGTTACTGTGGAGAGTGGCCGCCGACTCAAGCC CACCAACCTTGGCATAGTTCTGGTGCATGGTTACTACAAGATTGATGCAGAGCTGGTACTCCCCACCATC CGAAGCGCAGTGGAGAAGCAGCTGAATCTTATTGCCCAGGGCAAAGCTGACTACCACCAAGTTCTGGGCC ATACCCTGGATATCTTCAAGAGAAAGTTCCACTACTTCGTTGATTCTATTGCTGGCATGGACGAGTTGAT GGAGGTTTCCTTTTCACCCCTGGCTGCCACTGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCATCGATTT ATGAAGTACATCCAGGCCAAGCCAAGTCGCCTGCACTGCTCCCACTGTGATGAGACCTACACCCTCCCCC AGAATGGTACCATCAAGCTCTACAAGGAGCTGCGGTGCCCATTGGATGACTTTGAACTGGTCCTGTGGTC CTCAGGCTCCCGGGGCAAGAGCTACCCACTATGTCCCTACTGCTATAACCACCCACCCTTCCGAGACATG AAGAAAGGCATGGGCTGCAATGAGTGCACACACCCCACCTGCCAGCACTCACTGAGTATGCTGGGCATTG GCCAGTGCGTGGAATGCGAGAATGGAGTCCTTGTTCTGGACCCCACTTCTGGTCCCAAGTGGAAGGTGGC CTGCAATACATGCAATGTGGTGGCACACTGCTTTGAAAATGCCCACCGAGTACGGGTATCTGCTGACACC TGCAACACCTGCGAGGCTGCCTTGCTCGATGTAGACTTCAACAAGGCCAAGTCTCCCCTTCCTGGCAATG AGACACAGCACACAGGCTGCATCTTCTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTTAAGCATGCAGC CTCCTGCCACCCCATGCACCGAGGTGGGCCTGGAAGGAGGCAGGGTCGTGGGCGGGGCCGGGGCCGGAGA CCTCCAGGAAAGCCCAACCCTAGGCGGCCAAAAGACAAGATGTCAGCTCTGGCTGCTTACTTTGTATGAC AACCTATCTTTTCCCCACTCCAGACACATCGTGGTTATCTTTGAGCCACTGAAACCATTAAAATGCTTTT TATTTTGTATTCTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011624
• Insert Size: 2589 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC031723, AAH31723
• RefSeq Size: 2987 bp
• RefSeq ORF: 2589 bp
• Locus ID: 21976
• UniProt ID: Q9Z321
• Cytogenetics: 16 A3
• Summary: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.