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Nek8 (NM_080849) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210111
Nek8 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $840.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nek8
Synonyms 4632401F23Rik; b2b1449C; b2b1449Clo; jck
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210111 representing NM_080849
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGTACGAGCGGATCCGAGTGGTGGGAAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAA
AGGCCGACCAGAAGCTGGTGATCCTCAAGCAGATCCCAGTGGAACAGATGACCAAAGAAGAGAGGCAGGC
GGCCCAGAACGAGTGCCAGGTGCTCAAGCTGCTCAACCACCCCAACGTCATCGAGTACTATGAGAATTTC
CTAGAGGACAAGGCCCTCATGATTGCTATGGAGTACGCACCAGGTGGCACCTTGGCCGAGTTCATCCAGA
AGCGCTGCAACTCTCTGCTAGAGGAGGAGACGATCCTTCACTTCTTTGTGCAGATCCTGCTGGCTCTGCA
TCACGTGCATACACATCTCATCCTGCATCGGGACCTCAAGACACAGAACATCCTTCTTGACAAACACCGC
ATGGTGGTCAAGATCGGTGACTTTGGCATCTCTAAGATCCTCAGCAGCAAGAGCAAGGCCTACACGGTGG
TGGGTACACCCTGCTACATCTCCCCTGAGCTGTGTGAGGGCAAACCCTACAACCAGAAGAGTGACATCTG
GGCTCTGGGCTGTGTCTTATATGAGCTGGCCAGCCTCAAGAGGGCCTTCGAGGCTGCAAACTTGCCAGCC
CTGGTGCTGAAGATCATGAGTGGCACCTTCGCACCCATCTCTGACCGGTACAGCCCTGAGCTTCGCCAGC
TCGTCCTGAGTCTGCTTAGCCTGGAACCTGCACAGAGGCCACCCCTCAGCCACATCATGGCGCAGCCCCT
CTGCATCCGGGCCCTACTCAACATCCACACCGATGTGGGCAGCGTGCGCATGCGGAGGGCAGAGAAGTCC
CTGACCCCAGGACCACCTATAGCCTCTGGCAGCACAGGGAGCAGGGCCACCAGTGCCCGATGTAGGGGTG
TCCCCCGGGGACCTGTGAGACCGGCTATCCCACCGCCACTGTCTTCGGTCTACGCTTGGGGTGGTGGCCT
TAGCAGTCCCCTGAGGTTGCCAATGCTCAACACGGAGGTGGTCCAGGTAGCCGCTGGGCGCACGCAGAAG
GCTGGCGTTACGCGCTCTGGACGCCTCATCCTGTGGGAGGCCCCACCCCTGGGAGCTGGAGGCGGCACAC
TCCTTCCTGGAGCCGTGGAGCTGCCTCAGCCCCAGTTTGTCTCTCGTTTCCTGGAAGGCCAGTCAGGTGT
GACCATCAAGCATGTGGCCTGCGGGGACCTGTTCACCGCTTGCTTGACAGACCGAGGCATTATCATGACG
TTCGGCAGTGGCAGCAATGGGTGTTTAGGTCACGGCAACCTGACTGACATCAGCCAGCCCACCATTGTAG
AAGCCTTGCTGGGCTATGAGATGGTGCAGGTGGCCTGTGGGGCCTCTCATGTGCTGGCCCTGTCCACAGA
TGGAGAGTTATTCGCCTGGGGCAGAGGAGATGGTGGTAGACTGGGACTAGGCACCAGGGAGTCCCATAAC
TGCCCTCAGCAGGTGCCTGTGGCCCCAGGACAGGAAGCTCAGAGAGTTGTCTGTGGTATTGACTCTTCCA
TGATCCTCACCTCTCCAGGCAGGGTCCTGGCCTGTGGAAGTAACAGGTTCAACAAGCTAGGCTTGGACCA
CCTATCCCTGGACGAGGAGCCTGTTCCCTACCAGCAAGTGGAGGAGGCCCTGAGCTTCACACCACTGGGC
TCTGCACCTCTGGACCAGGAGCCCCTGCTGTGTGTGGACCTTGGCACTGCTCATTCAGCTGCTATCACTG
CTTCTGGGGACTGCTATACTTTTGGCAGCAATCAACATGGACAGCTGGGCACCAGTTCCCGCAGGGTCAG
CAGGGCACCCTGTCGGGTCCAAGGCCTGGAGGGCATCAAGATGGTGATGGTGGCCTGCGGGGATGCCTTC
ACTGTGGCCGTTGGGGCAGAGGGGGAAGTGTATTCTTGGGGCAAAGGAACCCGAGGCCGACTGGGAAGGA
GGGATGAGGATGCGGGGCTGCCTAGGCCAGTGCAGCTGGATGAGACTCATCCTTACATGGTGACTTCAGT
GTCCTGCTGCCATGGGAACACTCTGCTGGCTGTTCGATCAGTCACAGATGAACCAGTTCCCCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210111 representing NM_080849
Red=Cloning site Green=Tags(s)

MEKYERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENF
LEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHR
MVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPA
LVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKS
LTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTEVVQVAAGRTQK
AGVTRSGRLILWEAPPLGAGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMT
FGSGSNGCLGHGNLTDISQPTIVEALLGYEMVQVACGASHVLALSTDGELFAWGRGDGGRLGLGTRESHN
CPQQVPVAPGQEAQRVVCGIDSSMILTSPGRVLACGSNRFNKLGLDHLSLDEEPVPYQQVEEALSFTPLG
SAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAF
TVAVGAEGEVYSWGKGTRGRLGRRDEDAGLPRPVQLDETHPYMVTSVSCCHGNTLLAVRSVTDEPVPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080849
ORF Size 2094 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080849.2, NP_543125.1
RefSeq Size 2386 bp
RefSeq ORF 2097 bp
Locus ID 140859
UniProt ID Q91ZR4
Cytogenetics 11 46.74 cM
Summary This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans. provided by RefSeq, Jul 2008
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"NM_080849" in other vectors (4)
SKU Description Size Price
MC203539 Nek8 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), (10ug) 10 ug
$640.00
MR210111 Nek8 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$589.00 $640.00
MR210111L3 Lenti ORF clone of Nek8 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$940.00
MR210111L4 Lenti ORF clone of Nek8 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$940.00

Plasmid Map for MG210111

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210111
Circular map for MG210111

file-alt Citations

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