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Nek8 (NM_080849) Mouse Untagged Clone

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SKU
MC203539
Nek8 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $640.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nek8
Synonyms 4632401F23Rik; b2b1449C; b2b1449Clo; jck
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC070457
AGAAATGAGATGGAGAAGTACGAGCGGATCCGAGTGGTGGGAAGAGGTGCCTTCGGGATTGTGCACCTGT GCCTGCGAAAGGCCGACCAGAAGCTGGTGATCCTCAAGCAGATCCCAGTGGAACAGATGACCAAAGAAGA GAGGCAGGCGGCCCAGAACGAGTGCCAGGTGCTCAAGCTGCTCAACCACCCCAACGTCATCGAGTACTAT GAGAATTTCCTAGAGGACAAGGCCCTCATGATTGCTATGGAGTACGCACCAGGTGGCACCTTGGCCGAGT TCATCCAGAAGCGCTGCAACTCTCTGCTAGAGGAGGAGACGATCCTTCACTTCTTTGTGCAGATCCTGCT GGCTCTGCATCACGTGCATACACATCTCATCCTGCATCGGGACCTCAAGACACAGAACATCCTTCTTGAC AAACACCGCATGGTGGTCAAGATCGGTGACTTTGGCATCTCTAAGATCCTCAGCAGCAAGAGCAAGGCCT ACACGGTGGTGGGTACACCCTGCTACATCTCCCCTGAGCTGTGTGAGGGCAAACCCTACAACCAGAAGAG TGACATCTGGGCTCTGGGCTGTGTCTTATATGAGCTGGCCAGCCTCAAGAGGGCCTTCGAGGCTGCAAAC TTGCCAGCCCTGGTGCTGAAGATCATGAGTGGCACCTTCGCACCCATCTCTGACCGGTACAGCCCTGAGC TTCGCCAGCTCGTCCTGAGTCTGCTTAGCCTGGAACCTGCACAGAGGCCACCCCTCAGCCACATCATGGC GCAGCCCCTCTGCATCCGGGCCCTACTCAACATCCACACCGATGTGGGCAGCGTGCGCATGCGGAGGGCA GAGAAGTCCCTGACCCCAGGACCACCTATAGCCTCTGGCAGCACAGGGAGCAGGGCCACCAGTGCCCGAT GTAGGGGTGTCCCCCGGGGACCTGTGAGACCGGCTATCCCACCGCCACTGTCTTCGGTCTACGCTTGGGG TGGTGGCCTTAGCAGTCCCCTGAGGTTGCCAATGCTCAACACGGAGGTGGTCCAGGTAGCCGCTGGGCGC ACGCAGAAGGCTGGCGTTACGCGCTCTGGACGCCTCATCCTGTGGGAGGCCCCACCCCTGGGAGCTGGAG GCGGCACACTCCTTCCTGGAGCCGTGGAGCTGCCTCAGCCCCAGTTTGTCTCTCGTTTCCTGGAAGGCCA GTCAGGTGTGACCATCAAGCATGTGGCCTGCGGGGACCTGTTCACCGCTTGCTTGACAGACCGAGGCATT ATCATGACGTTCGGCAGTGGCAGCAATGGGTGTTTAGGTCACGGCAACCTGACTGACATCAGCCAGCCCA CCATTGTAGAAGCCTTGCTGGGCTATGAGATGGTGCAGGTGGCCTGTGGGGCCTCTCATGTGCTGGCCCT GTCCACAGATGGAGAGTTATTCGCCTGGGGCAGAGGAGATGGTGGTAGACTGGGACTAGGCACCAGGGAG TCCCATAACTGCCCTCAGCAGGTGCCTGTGGCCCCAGGACAGGAAGCTCAGAGAGTTGTCTGTGGTATTG ACTCTTCCATGATCCTCACCTCTCCAGGCAGGGTCCTGGCCTGTGGAAGTAACAGGTTCAACAAGCTAGG CTTGGACCACCTATCCCTGGACGAGGAGCCTGTTCCCTACCAGCAAGTGGAGGAGGCCCTGAGCTTCACA CCACTGGGCTCTGCACCTCTGGACCAGGAGCCCCTGCTGTGTGTGGACCTTGGCACTGCTCATTCAGCTG CTATCACTGCTTCTGGGGACTGCTATACTTTTGGCAGCAATCAACATGGACAGCTGGGCACCAGTTCCCG CAGGGTCAGCAGGGCACCCTGTCGGGTCCAAGGCCTGGAGGGCATCAAGATGGTGATGGTGGCCTGCGGG GATGCCTTCACTGTGGCCGTTGGGGCAGAGGGGGAAGTGTATTCTTGGGGCAAAGGAACCCGAGGCCGAC TGGGAAGGAGGGATGAGGATGCGGGGCTGCCTAGGCCAGTGCAGCTGGATGAGACTCATCCTTACATGGT GACTTCAGTGTCCTGCTGCCATGGGAACACTCTGCTGGCTGTTCGATCAGTCACAGATGAACCAGTTCCC CCCTGAGGCGACAGGATGATCCAAGAGACCACCTTTGAGTATTCTGAAAAATGCAGGTGCCCGAGGAATG ACTGTTGTCAGCGTCCTGGATTGTGTCGTCAATGGGGTAGAAAGGCCAGTGAAGTCTACTCTGTTCCCCC AAGTCCCTGTCTCCAAAGCTCTTTATCTTAGTAAATATCTGCAAGGCTCCAGTCTGGCAGGGGTCAACAG AAGGACGGCCTTCAAAACCTTTGCTAAAAAAAAAAAACGCACCTTTCTCCAACCACCCTCAGCCCAGTTA GAAAGTAAGTCCCTGACGGTGTCCTCTTGGAGACTCATGCCATGCTTTGGAATAGGGCAAGCCAGGGCTT GTTTGGACTGGTCATTAGGCTAGCCTAATCTCTGCATAGCTTGCAGCTGATAGGTTGGAGGGAGCCCCCT TTTCTATCTTACCCTGAGGCAAGGGTTTGCACAACCCCTGCGAAGGCACCCCTCCCCACACACACCTCTT AGGGAGGGGGTGCTGGAGCTATTTTTTTGTTTGCTGAGGAGATACTGCTGAAAGGTTGCCCTTTCCCCAG GCTATTCACCAGACCTAGACTGGGGAGAAAGGTAACCCGACCAATTTCTCAAAGGTTTCAAAGTATCACT AATAAAAATATCAAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_080849
Insert Size 2097 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC070457, AAH70457
RefSeq Size 2781 bp
RefSeq ORF 2097 bp
Locus ID 140859
UniProt ID Q91ZR4
Cytogenetics 11 46.74 cM
Summary This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_080849" in other vectors (4)
SKU Description Size Price
MG210111 Nek8 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$840.00
MR210111 Nek8 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$589.00 $640.00
MR210111L3 Lenti ORF clone of Nek8 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$940.00
MR210111L4 Lenti ORF clone of Nek8 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 8 (Nek8) 10 ug
$940.00

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