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Srrm4 (NM_026886) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209379
Srrm4 (tGFP-tagged) - Mouse RIKEN cDNA 1500001A10 gene (1500001A10Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $767.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Srrm4
Synonyms 1500001A10Rik; B230202K19Rik; bv; mKIAA1853; nSR100
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209379 representing NM_026886
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAGCCTGCAGCAGGGCGAGAAACAGCTGTTTGAGAAGTTCTGGAAAGGGACCTTCAAAGCAGTGG
CCACTCCGCGTCCCGAGAGCATCATCGTCGCCAGTATCACAGCCCGCAAGCCGATGCCAAGGACAGAGCC
CCAGAGCAGTCTCTTGCTCCCAGATCAGGATGGACCTTCAGAAAAGCTGGGCCAACATCTGGCCCCTGAG
GCCCTGGGTACCAACAGCTGGGGAAGAGAGAAGGCCTGCAGGGAACTGGATCCTGCCAGAGCACACAGTG
CCTCTCAAGACAGAGACCCGACACCACCACCTTCCTCCAGGGGGAAAAAGAAAAAGAAGAAATCAACCAG
GAAAAAGAGGAGGAGGTCCCCGTCCTACAGCCCATCACCAGTCAAGAAGAAGAAGAAGAAAAGCTCCAAG
AAACATAAGCGACACAGGTCTTTCTCCAAGAAAAGGAGACACAGCTCCTGTAGCCCTAAAAGCAAAAGAC
GGGAAGAGAAGAGGCACAAAAAACAATCTCGAAGCCGGAAGTCTCACCGACATAGACATCACCGATGCCC
CTCAAGGTCCCAGAGTTCGGAACTGCGCTCCCCCAGCTGCGAGAGCAGGCATCGAGGTCGGTCCCCTGAG
GAAGGGCGGAAATCCCGCCGAACACACTCCCGCCGCTGCTCCAAGAACCACTGCAAGGTCAGCCCAGACG
CCCGGTCCAGCCACCTGCCCAGCCAGCCCCTCCCGAGGCTCGGCTTCCTGTCAGCCAGGGGTGTAATCAC
TGGGTCGGGGTCCGCGGCTGACCTCTTTAGCAAATCGGCCAGCCCTCTCGCCGCCACCCGAGGGCGCTCG
CAGGAGTACGACTCGGGCAATGACACGTCCTCGCCGCCCTCCACGCAAACCAGCTCGGCCAGGTCTCGGG
GCCAGGAGAAGGGGAGTCCCGGTGGGGATCTAAGCAAGAGTCGAGACCTCAACTGTGGCAACACCTCCGA
TTCAGGGAACTCCTTCACCACCTCCTCCCCCCAGAACAAGGGGGCTGTCTTGGAGACTGTCTCTCCCGCC
TGCAGGAGCAGAGAGTCAAGAGGGTTTCAGTCCCCATGTCTCCAGTGTGCAGAGGTGAAGAAGTCTAGTT
TGGTCCCATCCACAGCCCGGAGCTCCCCCATCAAAGAGTGCTCCCGTAGCTCCTCCTACACCAGCACTCG
ATCCTCCAGTCCCTCATCCAGGTCCCCAAACCCCAGAGCTTCCCCCAGGTACACCCGGAGTCGGTCCACC
TCCTCTGAAAAAAGGTCCTACTCTCGCTCTCCCAGCTATTCGTCCAAGTCTGGCAAGAGGAGCCCACCTA
GCAGAAGCTCCAGGTCTCGTCGAAGTCCCAGCTACTCTCGCTACAGCCCCAGCAGGGAACGGGACCTAAA
GTATGGTGAGAAAGAACCACAGCCCCGAGAACGTGCGCGCAGGAGGCGACGGTCCTACTCACCCATGAGG
AAGCGCAGGAGAGACTCCCCAAGCCACCTGGAGGCTCGGAGGATAACCAGTGCCCGCAAACGCCCCATCC
CCTACTACCGACCCAGCCCCTCCTCGTCCAGCAGCCTCAGCAGCGCCTCCTCCTGGTACAGCAGCAGCAG
CAGCAGCAGCAGCAGTAGCAGCCGCTCCCCTAGCCGCAGTTACTCCCGGAGCCGGAGTCCCAGTCGGAGC
CACAGCAGCCGGAGCCAGACCCGGAGCAGGACGCGGACCAGCCGCAGCTCCAGTTCTCGCAGCCTCAGCC
TGGGCTCCCGGAGCCGCAGTCGGAACCGGAGCCTGAGCTACAGCTCGGCAGAAAGCTATGCCAGCACGAG
GCGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209379 representing NM_026886
Red=Cloning site Green=Tags(s)

MASLQQGEKQLFEKFWKGTFKAVATPRPESIIVASITARKPMPRTEPQSSLLLPDQDGPSEKLGQHLAPE
ALGTNSWGREKACRELDPARAHSASQDRDPTPPPSSRGKKKKKKSTRKKRRRSPSYSPSPVKKKKKKSSK
KHKRHRSFSKKRRHSSCSPKSKRREEKRHKKQSRSRKSHRHRHHRCPSRSQSSELRSPSCESRHRGRSPE
EGRKSRRTHSRRCSKNHCKVSPDARSSHLPSQPLPRLGFLSARGVITGSGSAADLFSKSASPLAATRGRS
QEYDSGNDTSSPPSTQTSSARSRGQEKGSPGGDLSKSRDLNCGNTSDSGNSFTTSSPQNKGAVLETVSPA
CRSRESRGFQSPCLQCAEVKKSSLVPSTARSSPIKECSRSSSYTSTRSSSPSSRSPNPRASPRYTRSRST
SSEKRSYSRSPSYSSKSGKRSPPSRSSRSRRSPSYSRYSPSRERDLKYGEKEPQPRERARRRRRSYSPMR
KRRRDSPSHLEARRITSARKRPIPYYRPSPSSSSSLSSASSWYSSSSSSSSSSSRSPSRSYSRSRSPSRS
HSSRSQTRSRTRTSRSSSSRSLSLGSRSRSRNRSLSYSSAESYASTRR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026886
ORF Size 1824 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026886.3, NP_081162.1
RefSeq Size 7476 bp
RefSeq ORF 1827 bp
Locus ID 68955
UniProt ID Q8BKA3
Cytogenetics 5 F
Summary Splicing factor specifically required for neural cell differentiation. Acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. Required to promote the inclusion of neural-specific exon 10 in nPTB/PTBP2, leading to increased expression of neural-specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in neuron extracts (PubMed:19737518). Promotes alternative splicing of REST transcripts to produce REST isoform 2 (REST4) with greatly reduced repressive activity, thereby activating expression of REST targets in neural cells (PubMed:21884984). Plays an important role during embryonic development as well as in the proper functioning of the adult nervous system. Regulates alternative splicing events in genes with important neuronal functions (PubMed:25838543).UniProtKB/Swiss-Prot Function
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"NM_026886" in other vectors (4)
SKU Description Size Price
MC219631 Srrm4 (untagged) - Mouse serine/arginine repetitive matrix 4 (Srrm4), (10ug) 10 ug
$624.00
MR209379 Srrm4 (Myc-DDK-tagged) - Mouse serine/arginine repetitive matrix 4 (Srrm4) 10 ug
$289.00 $567.00
MR209379L3 Lenti ORF clone of Srrm4 (Myc-DDK-tagged) - Mouse serine/arginine repetitive matrix 4 (Srrm4) 10 ug
$867.00
MR209379L4 Lenti ORF clone of Srrm4 (mGFP-tagged) - Mouse serine/arginine repetitive matrix 4 (Srrm4) 10 ug
$867.00

Plasmid Map for MG209379

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209379
Circular map for MG209379

file-alt Citations

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