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Ppp1r16b (NM_153089) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208959
Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $730.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppp1r16b
Synonyms ANKRD4; C130078N17Rik; TIMAP; Wdt4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208959 representing NM_153089
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCACGTGGACCTGCTCACAGAGCTGCAGCTGCTGGAGAAGGTGCCTACGCTGGAGCGCCTGC
GCGCAGCCCAGAAGCGCCGGGCTCAGCAGCTGAAGAAGTGGGCACAGTACGAGCAGGACCTGCTTCATCG
CAAGCGCAAGCATGAGCGGAAGCGCAGCACGGGTGGACGTCGCAAGAAGGTGTCCTTCGAGGCCAGTGTA
GCGTTGCTGGAAGCCTCACTGAGGAATGACGCCGAAGAAGTTCGCTACTTCCTGAAGAACAAGGTTAGCC
CTGATTTGTGCAATGAGGATGGACTTACAGCCTTGCACCAGTGCTGCATTGACAACTTTGAAGAGATTGT
CAAGCTGCTGCTGTCCCACGGTGCGAACGTGAATGCCAAGGACAACGAGCTGTGGACGCCCTTGCACGCG
GCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCTGACTTGCTTGCTGTCA
ACTCTGATGGAAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGGATGTCATCGAGACCTGCAT
GGCATACCAAGGCATCACACAGGAGAAAATCAACGAGATGCGAGCAGCCCCTGAGCAGAAGATGATTTCA
GACATCCACTGCATGATAGCAGCGGGCCAGGATCTCGACTGGATCGATGGCCAGGGTGCCACACTGCTAC
ACATAGCTGGAGCCAATGGATATCTGCGAGCAGCCGAGCTCCTCCTGGACCATGGAGTGCGTGTGGATGT
GAAGGACTGGGATGGCTGGGAGCCTCTGCATGCAGCAGCCTTCTGGGGACAGATGCCAATGGCAGAGCTG
CTGGTGTCCCATGGAGCTAGTCTCAGTGCCAGGACATCCATGGATGAGATGCCAATAGACCTGTGTGAGG
AGGAGGAATTCAAGGTCCTGCTGTTGGAGCTGAAACACAAGCATGATGTGATCATGAAGTCGCAGCTGAG
GCACAAGTCGTCCCTGAGCCGGAGGACATCCAGCGCGGGCAGCCGTGGGAAGGTGGTGCGTCGAGCCAGC
CTGTCAGACAGAACCAATCTATACCGGAAAGAATATGAGGGCGAGGCCATCTTGTGGCAGCAGCGGAGTG
CAGCTGAGGACCAGCGGACTTCAACCTACAATGGAGACATCAGGGAGACCAGGACAGACCAGGAGAACAA
GGACCCGAACCCCAGGCTGGAGAAGCCCGTGCTGCTCTCAGAATTCTCTACCAAGATCTCACGGGGAGAA
CTGGATGGGCCTGTGGAGAATGGCCTCCGGGCTCCTGTCAGCACTTACCAGTATGCGCTGGCCAATGGGG
ACATATGGAAGATGCACGAGATGCCTGACTACAGCATGGCCTACGGCAACCCTGGCGTGGCTGATGTCCC
CCCACCCTGGAGTGGCTTCAAGGAGCAGAGCCCCCAAACGCTCCTGGAGCTGAAGAGGCAGAGGGCCGCA
GCCAAGCTGCTCAGTCACCCATTCTTAAGCACCCACCTGGGCAGCAGCGTGGCCAGGTCGGGGGAGAGCA
GCAGCGAAGGCAAGGCCCCCTTGATTGGAGGCAGAACTTCCCCCTACAGCAGCAACGGGACCTCGGTGTA
TTATACAGTCACCAGCGGAGATCCCCCGCTCTTGAAGTTCAAGGCCCCCATGGAGGAGATGGAGGAGAAA
GTTCACGGCTGCTGCCGGATCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208959 representing NM_153089
Red=Cloning site Green=Tags(s)

MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLLHRKRKHERKRSTGGRRKKVSFEASV
ALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHA
AATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRAAPEQKMIS
DIHCMIAAGQDLDWIDGQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMPMAEL
LVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRAS
LSDRTNLYRKEYEGEAILWQQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFSTKISRGE
LDGPVENGLRAPVSTYQYALANGDIWKMHEMPDYSMAYGNPGVADVPPPWSGFKEQSPQTLLELKRQRAA
AKLLSHPFLSTHLGSSVARSGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPMEEMEEK
VHGCCRIS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153089
ORF Size 1704 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153089.2
RefSeq Size 3228 bp
RefSeq ORF 1707 bp
Locus ID 228852
UniProt ID Q8VHQ3
Cytogenetics 2 H1
Summary Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_153089" in other vectors (5)
SKU Description Size Price
MC219120 Ppp1r16b (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2, (10ug) 10 ug
$583.00
MG219271 Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 16B (Ppp1r16b) transcript variant 2, (10ug) 10 ug
$730.00
MR219271 Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$289.00 $530.00
MR219271L3 Lenti ORF clone of Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$830.00
MR219271L4 Lenti ORF clone of Ppp1r16b (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$830.00

Plasmid Map for MG208959

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208959
Circular map for MG208959

file-alt Citations

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