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Ppp1r16b (NM_153089) Mouse Untagged Clone

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SKU
MC219120
Ppp1r16b (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP tGFP DDK
AAV Particle:
DDK
  $583.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ppp1r16b
Synonyms ANKRD4; C130078N17Rik; TIMAP; Wdt4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219120 representing NM_153089
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCACGTGGACCTGCTCACAGAGCTGCAGCTGCTGGAGAAGGTGCCTACGCTGGAGCGCCTGC
GCGCAGCCCAGAAGCGCCGGGCTCAGCAGCTGAAGAAGTGGGCACAGTACGAGCAGGACCTGCTTCATCG
CAAGCGCAAGCATGAGCGGAAGCGCAGCACGGGTGGACGTCGCAAGAAGGTGTCCTTCGAGGCCAGTGTA
GCGTTGCTGGAAGCCTCACTGAGGAATGACGCCGAAGAAGTTCGCTACTTCCTGAAGAACAAGGTTAGCC
CTGATTTGTGCAATGAGGATGGACTTACAGCCTTGCACCAGTGCTGCATTGACAACTTTGAAGAGATTGT
CAAGCTGCTGCTGTCCCACGGTGCGAACGTGAATGCCAAGGACAACGAGCTGTGGACGCCCTTGCACGCG
GCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCTGACTTGCTTGCTGTCA
ACTCTGATGGAAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGGATGTCATCGAGACCTGCAT
GGCATACCAAGGCATCACACAGGAGAAAATCAACGAGATGCGAGCAGCCCCTGAGCAGAAGATGATTTCA
GACATCCACTGCATGATAGCAGCGGGCCAGGATCTCGACTGGATCGATGGCCAGGGTGCCACACTGCTAC
ACATAGCTGGAGCCAATGGATATCTGCGAGCAGCCGAGCTCCTCCTGGACCATGGAGTGCGTGTGGATGT
GAAGGACTGGGATGGCTGGGAGCCTCTGCATGCAGCAGCCTTCTGGGGACAGATGCCAATGGCAGAGCTG
CTGGTGTCCCATGGAGCTAGTCTCAGTGCCAGGACATCCATGGATGAGATGCCAATAGACCTGTGTGAGG
AGGAGGAATTCAAGGTCCTGCTGTTGGAGCTGAAACACAAGCATGATGTGATCATGAAGTCGCAGCTGAG
GCACAAGTCGTCCCTGAGCCGGAGGACATCCAGCGCGGGCAGCCGTGGGAAGGTGGTGCGTCGAGCCAGC
CTGTCAGACAGAACCAACCTATACCGGAAAGAATATGAGGGCGAGGCCATCTTGTGGCAGCAGCGGAGTG
CAGCTGAGGACCAGCGGACTTCAACCTACAATGGAGACATCAGGGAGACCAGGACAGACCAGGAGAACAA
GGACCCGAACCCCAGGCTGGAGAAGCCCGTGCTGCTCTCAGAATTCTCTACCAAGATCTCACGGGGAGAA
CTGGATGGGCCTGTGGAGAATGGCCTCCGGGCTCCTGTCAGCACTTACCAGTATGCGCTGGCCAATGGGG
ACATATGGAAGATGCACGAGATGCCTGACTACAGCATGGCCTACGGCAACCCTGGCGTGGCTGATGTCCC
CCCACCCTGGAGTGGCTTCAAGGAGCAGAGCCCCCAAACGCTCCTGGAGCTGAAGAGGCAGAGGGCCGCA
GCCAAGCTGCTCAGTCACCCATTCTTAAGCACCCACCTGGGCAGCAGCGTGGCCAGGTCGGGGGAGAGCA
GCAGCGAAGGCAAGGCCCCCTTGATTGGAGGCAGAACTTCCCCCTACAGCAGCAACGGGACCTCGGTGTA
TTATACAGTCACCAGCGGAGATCCCCCGCTCTTGAAGTTCAAGGCCCCCATGGAGGAGATGGAGGAGAAA
GTTCACGGCTGCTGCCGGATCTCCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_153089
Insert Size 1707 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153089.4, NP_694729.1
RefSeq Size 6370 bp
RefSeq ORF 1707 bp
Locus ID 228852
UniProt ID Q8VHQ3
Cytogenetics 2 H1
Summary Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_153089" in other vectors (5)
SKU Description Size Price
MG208959 Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b) 10 ug
$730.00
MG219271 Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 16B (Ppp1r16b) transcript variant 2, (10ug) 10 ug
$730.00
MR219271 Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$289.00 $530.00
MR219271L3 Lenti ORF clone of Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$830.00
MR219271L4 Lenti ORF clone of Ppp1r16b (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 10 ug
$830.00

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