Asna1 (NM_019652) Mouse Tagged ORF Clone

SKU
MG205259
Asna1 (tGFP-tagged) - Mouse arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 (Asna1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$657.00
3 Weeks*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asna1
Synonyms 1810048H22Rik; ArsA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205259 representing NM_019652
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGGGGTGGCCGGGTGGGGGGTTGAAGCAGAAGAGTTCGAGGATGCACCTGATGTGGAGCCGC
TGGAACCCACGCTTAGCAATATCATCGAGCAGCGTAGCCTTAAGTGGATCTTTGTCGGGGGCAAGGGTGG
CGTTGGTAAGACCACCTGCAGCTGCAGCCTGGCAGTCCAGCTGTCGAAGGGACGTGAGAGTGTTCTGATC
ATTTCCACAGACCCAGCTCACAACATCTCAGATGCTTTTGACCAGAAGTTCTCCAAGGTGCCTACCAAGG
TCAAAGGCTATGACAACCTCTTTGCTATGGAGATAGACCCCAGCCTGGGCGTTGCAGAGCTCCCCGACGA
GTTCTTCGAGGAAGACAACATGCTGAGCATGGGCAAGAAGATGATGCAGGAGGCCATGAGCGCCTTCCCT
GGCATCGATGAAGCCATGAGTTATGCTGAGGTCATGAGGCTGGTGAAAGGCATGAACTTCTCAGTGGTAG
TGTTCGACACAGCACCCACCGGCCATACACTCAGGCTCCTGAACTTCCCGACCATCGTGGAGAGGGGCCT
GGGCCGCCTGATGCAGATCAAGAACCAGATCAGCCCCTTCATCTCACAGATGTGCAACATGCTGGGCCTG
GGGGACATGAATGCTGACCAACTGGCCTCCAAGCTAGAAGAGACGTTGCCTGTCATCCGATCTGTTAGCG
AACAGTTCAAGGACCCTGAACAGACAACGTTCATCTGTGTGTGCATCGCCGAATTCTTGTCCTTGTACGA
GACGGAGCGGCTGATCCAGGAGCTGGCTAAGTGCAAGATCGACACCCACAACATCATCGTCAACCAGCTT
GTCTTCCCTGACCCTGAGAAACCCTGCAAGATGTGTGAGGCCCGACACAAGATCCAGGCCAAGTACCTGG
ACCAGATGGAAGACCTATATGAAGACTTTCACATTGTAAAGCTGCCACTGTTACCTCACGAGGTTCGGGG
AGCCGACAAAGTCAACACCTTCTCTGCCCTCCTCCTGGAGCCCTACAAGCCCCCCAGCACCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205259 representing NM_019652
Red=Cloning site Green=Tags(s)

MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLI
ISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFP
GIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGL
GDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQL
VFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSTQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_019652
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019652.1, NP_062626.1
RefSeq Size 1280 bp
RefSeq ORF 1047 bp
Locus ID 56495
UniProt ID O54984
Cytogenetics 8 C3
Summary ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.