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Tmem16a (BC006062) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205066
Tmem16a (tGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem16a
Synonyms Tmem16a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205066 representing BC006062
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCA
AGCAGCTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAA
GCTGCGCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTC
AACCTCGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCC
TGTTTGTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGA
TGCCAAAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTAT
AACATCCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACT
TCATCCCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACAC
GCTCTCTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTAT
GAGGTTCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCA
AAGACTTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCAT
GAGTGACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAG
GTTCTCATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGA
AGGAGAAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAG
CCCAGTCCCCAGCTACGAGTACCATGGGGACGCACTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205066 representing BC006062
Red=Cloning site Green=Tags(s)

MEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDF
NLEPFAGLTPEYMEMIIQFGFVTLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWY
NILRGVGKLAVIINAFVISFTSDFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGY
EVQICRYKDYREPPWSEHKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEK
VLMVELFMREEQGKQQLLDTWMEKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC006062
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006062, AAH06062
RefSeq Size 2473 bp
RefSeq ORF 1019 bp
Locus ID 101772
Cytogenetics 7 F5
Summary Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC006062" in other vectors (4)
SKU Description Size Price
MC200539 Tmem16a (untagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765), (10ug) 10 ug
$457.00
MR205066 Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$289.00 $457.00
MR205066L3 Lenti ORF clone of Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$757.00
MR205066L4 Lenti ORF clone of Tmem16a (mGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$757.00

Plasmid Map for MG205066

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205066
Circular map for MG205066

file-alt Citations

Binding of plasma membrane lipids recruits the yeast integral membrane protein Ist2 to the cortical ER
Fischer, Temmerman, Ercan et al
Traffic (2009) 10 (8), 1084-97 DOI: 10.1111/j.1600-0854.2009.00926.x
Cited by 56
AI Summary: In this paper, OriGene's anti-GFP antibody (Cat# TA150041) was used to detect GFP-tagged proteins, facilitating the analysis of Ist2 localization and recruitment to the cortical ER.
Showing 1-1 of 1 papers.
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