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Tmem16a (BC006062) Mouse Untagged Clone

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SKU
MC200539
Tmem16a (untagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tmem16a
Synonyms Tmem16a
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC006062
CCCACGCGTCCGGTCGCCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCC GCTCTTTCCGGATGGAGGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCAT TATGCTGGGCAAGCAGCTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATC CGCTACCTGAAGCTGCGCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATG AGGTGGACTTCAACCTCGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGG CTTTGTCACCCTGTTTGTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAG ATCCGCCTGGATGCCAAAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCG GCATCTGGTATAACATCCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTT CACGTCTGACTTCATCCCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTC GTCAATCACACGCTCTCTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGG ACCTGGGCTATGAGGTTCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTA TGACATCTCCAAAGACTTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTG GTGATGTTCATGAGTGACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCC ACAAAGAGAAGGTTCTCATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACAC ATGGATGGAGAAGGAGAAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCA GGCGACGGCAGCCCAGTCCCCAGCTACGAGTACCATGGGGACGCACTGTAGCCATGATGACAGGCCAGCA GGCCAGCAGGCTTCTGACCATCACCAAGTGACCACTCGGCTTCCTCCAAAGCCAGCACTTCTAGCCCCAC CAGGGCCTATGGCTCTTGTGTCGTCTGGACGTGTGGAGGAGCACTCTGATGCGACAACGGTCTTCTTTCT TCTTTCTGTTTCTTCTCATTTTTGCACAAGGCCATTATGCAGGGAAGTTTTTATCTTTAGTATTCAATGC CAAGGATGGCTGGTGATGGGGATGAACAGGGTGAGGCATGTTCCACAGACATATGGAGGAATATAGAGCC CCCCTGTGGTGGTAGGGAGCCAAGTCAGCATCCTTAGTCTGAAGGATGCCCTGGGATAGATGCAGAATGG TACCTAGGAACCTGCTGACATTTTGCAGAAGGGTGAACAACTCAGACTCAGATTCCCAAAGATGTCACCT GCTCTAGCTGGACAAGCTGGAAGACTCCACTGTCTTTCCTTCTGGGAGCTCAGAAGTTCAGACCTGGGGT CAGAAACAGAGGTGCCTGACCACCCACACAGCACACACACATGGTGGCTTCACATTGTACTCCAGTAACG GAGGAGTACAGGGGCTCCCAGTTGTCCAGTACCCTTGGGGTCCCTGGCAGGCTGCACACAAGCCACTCAA GGGCCCCTCACATGAAGCTGATGTCCTCCTTGTGAGACAAGCTGTCGTGGCAATCTCTAAACAGGCTGGA GGGCTTCACCAAGCCCTGGGGCCTCTGTGGGTTCATTTTTTTTTTTTTTTGCTTTTTAAACCATCTATTG GTTTTTGTCTAAAAATCAATAATGTGATGGATTTGAGGGATTTTCTTTCTGTAGCTGAAGGTGGAAGGAG TTTATTTCATTAGTTAACCAAATACTGAGAGGAAATTAAAATATCATTACTACCAAAGATTTTTCTCAAT AAAAGCTTCTATTTTGGTAACACACCTATATTTTCACTCGTGGGGATGAGACCCTCAGCACTTACCATTG TAATAGCTTCTAAAACAAGCCTCATGGGCCAGCTTCATTTGATGTGCTAGCCAGCTGTGCAAGGCCTCTC GGCACCATTGCCAGAAGTGTCTCTCTCGCCCAAGCCGAAGAAGGCAATTCCCTGCACTCGTCTTAGGGCA AGGCAGACAGTGCTGTAGTTCGTGAGCTGGGGTCCGAATCTCAGGCTTCCAAGGTTTGCACTGTTTGCTA AAATCATGAGACCTGATGCTTCTATTTTCTCTTGTGCAGTGATGTGGTTTCCAATGATGTTTTGTATATA GCTACTGTATCCAAAATTAGATAATTACCAAAAATTCTTTTACAGAAACCATTTTTAAAAAACAACAAAA AAAACTAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN BC006062
Insert Size 1020 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC006062, AAH06062
RefSeq Size 2473 bp
RefSeq ORF 1020 bp
Locus ID 101772
Cytogenetics 7 F5
Summary Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC006062" in other vectors (4)
SKU Description Size Price
MG205066 Tmem16a (tGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$657.00
MR205066 Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$289.00 $457.00
MR205066L3 Lenti ORF clone of Tmem16a (Myc-DDK-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$757.00
MR205066L4 Lenti ORF clone of Tmem16a (mGFP-tagged) - Mouse transmembrane protein 16A (cDNA clone MGC:8241 IMAGE:3591765) 10 ug
$757.00

file-alt Citations

Knockdown of TMEM16A suppressed MAPK and inhibited cell proliferation and migration in hepatocellular carcinoma
Deng, Yang, Chen et al
Onco Targets Ther (2016) 9, 325-33 DOI: 10.2147/OTT.S95985
AI Summary: OriGene contributed TMEM16A siRNA and negative siRNA products, which were used to knock down TMEM16A expression in SMMC-7721 hepatocellular carcinoma cells to study its effects on cell proliferation, migration, and MAPK signaling.
Snippet: 37°C in 5% CO 2 . Cell transfection. For knockdown of TMEM16A, the TMEM16A siRNA and negative siRNA (50 nM) were synthesized and purified by OriGene (OriGene, Rockville, MD, USA). SMMC-7721 cells were seeded ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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