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Mettl7a1 (NM_027334) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG203043
Mettl7a1 (tGFP-tagged) - Mouse methyltransferase like 7A (Mettl7a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $500.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mettl7a1
Synonyms 2210414H16Rik; 3300001H21Rik; Aam-B; Mettl7a; UbiE1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203043 representing NM_027334
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCTTGCCGTCCTGGCCCTGCGGCTGGTGGTCTGTACCCTGGCGCTTCCCATGTTTCTGCTGAACC
TTCTAGGCTTGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCTGAAGCGATTCTCCGTGATGTA
CAATGAGCAGATGGCGAGCCAAAAGCGGGAGCTCTTCAGCAATCTGCAGGAGTTCGCCGGCCCCTCGGGG
AAGCTGACTCTGCTGGAGGTGGGCTGCGGCACCGGAGCCAACTTCAAGTTCTATCCCCCCGGGTGCAGGG
TCACTTGTATCGACCCCAACCCCAACTTCGAGAAGTTCTTGTTCAAGAGCGTCGCAGAGAACCGGCAGCT
GCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGATGGCTCTGTGGACGTG
GTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGTGAGGTGTGCCGAGTGC
TGAAGCCGGGAGGGGCTTTTTACTTCATGGAACACGTGGCAGATGAACGGTCTACCTGGAATTACTTCTG
GCAGCAGGTCCTGGATCCTGTCTGGTTCCTTTTTTTTGATGGATGCAATCTGACGAGAGAGAGCTGGAAG
ACCATAGAGCAGGCCAGCTTCTCGAAGCTAAAGCTACAGCACATCCAGGCCCCCCTCTCCTGGACATTGG
TGCGGCCCCACATCTATGGCTATGCTGTGAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203043 representing NM_027334
Red=Cloning site Green=Tags(s)

MTLAVLALRLVVCTLALPMFLLNLLGLWSWVCKKCFPYFLKRFSVMYNEQMASQKRELFSNLQEFAGPSG
KLTLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLFKSVAENRQLQFERFVVAAGEDMHQVTDGSVDV
VVCTLVLCSVKNQEKILREVCRVLKPGGAFYFMEHVADERSTWNYFWQQVLDPVWFLFFDGCNLTRESWK
TIEQASFSKLKLQHIQAPLSWTLVRPHIYGYAVK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027334
ORF Size 732 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027334.3, NP_081610.2
RefSeq Size 1883 bp
RefSeq ORF 735 bp
Locus ID 70152
Cytogenetics 15 F1
MW 28.1 kDa
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"NM_027334" in other vectors (4)
SKU Description Size Price
MC205948 Mettl7a1 (untagged) - Mouse methyltransferase like 7A1 (Mettl7a1), (10ug) 10 ug
$300.00
MR203043 Mettl7a1 (Myc-DDK-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$289.00 $300.00
MR203043L3 Lenti ORF clone of Mettl7a1 (Myc-DDK-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$600.00
MR203043L4 Lenti ORF clone of Mettl7a1 (mGFP-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$600.00

Plasmid Map for MG203043

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG203043
Circular map for MG203043

file-alt Citations

Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis
Krahmer, Najafi, Schueder et al
Dev. Cell (2018) 47 (2), 205-221.e7 DOI: 10.1016/j.devcel.2018.09.017
AI Summary: OriGene's pCMV6-AC-Mettl7A-GFP plasmid (MG203043) was used as the template for generating a mutant Mettl7A construct, enabling the study of protein localization and function in hepatic steatosis.
Snippet: ;Mettl7A S216A was generated by site-directed mutagenesis using a site-directed mutagenesis kit (Q5 Site-Directed Mutagenesis kit, NEB) with the following primers: Fwd 5’AGAGCAGGCCgcaTTCTCGAAGCTAAAG3’, Rev 5’ATGGTCTTCCAGCTCTCTC3’ from the plasmid pCMV6-AC-Mettl7A-GFP (MG203043, Origene)
Showing 1-1 of 1 papers.
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