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Mettl7a1 (NM_027334) Mouse Untagged Clone

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SKU
MC205948
Mettl7a1 (untagged) - Mouse methyltransferase like 7A1 (Mettl7a1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mettl7a1
Synonyms 2210414H16Rik; 3300001H21Rik; Aam-B; Mettl7a; UbiE1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC055034
CTTTCTCCTCTACTCGCAAAGATGACGCTTGCCGTCCTGGCCCTGCGGCTGGTGGTCTGTACCCTGGCGC TTCCCATGTTTCTGCTGAACCTTCTAGGCTTGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCT GAAGCGATTCTCCGTGATGTACAATGAGCAGATGGCGAGCCAAAAGCGGGAGCTCTTCAGCAATCTGCAG GAGTTCGCCGGCCCCTCGGGGAAGCTGACTCTGCTGGAGGTGGGCTGCGGCACCGGAGCCAACTTCAAGT TCTATCCCCCCGGGTGCAGGGTCACTTGTATCGACCCCAACCCCAACTTCGAGAAGTTCTTGTTCAAGAG CGTCGCAGAGAACCGGCAGCTGCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTG ACCGATGGCTCTGTGGACGTGGTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTC TGCGTGAGGTGTGCCGAGTGCTGAAGCCGGGAGGGGCTTTTTACTTCATGGAACACGTGGCAGATGAACG GTCTACCTGGAATTACTTCTGGCAGCAGGTCCTGGATCCTGTCTGGTTCCTTTTTTTTGATGGATGCAAT CTGACGAGAGAGAGCTGGAAGACCATAGAGCAGGCCAGCTTCTCGAAGCTAAAGCTACAGCACATCCAGG CCCCCCTCTCCTGGACATTGGTGCGGCCCCACATCTATGGCTATGCTGTGAAATAGAGTGAGGTGGTGGG AGAGATCCGGTCTGCTCCGAGCTGGTTCCAGGCTTCCGTTTTAGATGTAAAATTCTCACTGGGAGGGGTG AAAATCAAATTCATCCCTACAGGTTTCTGTTTAGCCATTTTCTATGTTGTTTCTTACTGAATCAAAACCA AATCAGAGCAGCTTTGAACTCTCCCTCCAAAGGGACCAAAGGTCTCTCATAGGCCTTGGAAAAGGAAGCA GTGGGGGAGACTTACAGCTTGCAAACCGAATAAATAAATCCCAGAAAATGGGAAATCCACGGCTGACTAA CACAGGAACACCTCCTTCCCTCCCCCAACCCGGTAAGAAGGAATCAGAACCCACTTGCTATCCTGGTGTT ACTAGTCTGCTTTGGCTGCCACCTGTGCTCTGGCCAGTGGCTCTCTGTCCTTTGGCTCAGGGTTTTGCCA ATGGCTGCTGCATTGTCCTGCACGCCACAGCTCGTATAAGTGCACCTGACACACCAGCGTCATCACAGAA CTCCACAGAACAGATGGGAATTCTCTGTTTAATTTCCCATCTTATCTAGCACATACTTTGCTGAATATAA TTACCGTAGGCAGTAGAAGGCCACTATAGTTAGGGAAATGGGTACAGTACGTGGGGAACCCTAAAAGTAA TGCACGAGAGTAGTTACCAAGGACTCGGGGTGCGGGGGGGAGGGATTCTTCTGATCAAATATCACTGGTC CTGAGTGGGTGTGGTGGTTCAGCCTTAGTCCCAGCACTCAGGAGGCAGAGGCAGAGGGGCAGAGAGAGAG GCAGAGAGAGAGGCAGAGAGACAGAGGCAGAGACAGGTAGATATCCTTGAGTACAGCCTGGTCTACAGAT CAAGTTCCAGGACCGCCAGGGCTACACAAAGAAACCCTGTATTGAAGAAGAAGAAAAAAATTCCACTGGT CCTGAAACAAAACCATGGATATCATTGTGTTCATACAGAGAGCAAGTCTGGGTGGGCCCAAGGGTTTCAG CTGCATTTGTATGTATGGATCTAAACAAATGTATATTTTTCAAGATGATTTTGCAGAAGTTCTATTTTAA ATGTTGATCAATAAATTCATATTCATTTAATAAATGAAAATAAATGGTTTCTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_027334
Insert Size 735 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC055034, AAH55034
RefSeq Size 1960 bp
RefSeq ORF 735 bp
Locus ID 70152
Cytogenetics 15 F1
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Documents
Resources
"NM_027334" in other vectors (4)
SKU Description Size Price
MG203043 Mettl7a1 (tGFP-tagged) - Mouse methyltransferase like 7A (Mettl7a) 10 ug
$489.00 $500.00
MR203043 Mettl7a1 (Myc-DDK-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$289.00 $300.00
MR203043L3 Lenti ORF clone of Mettl7a1 (Myc-DDK-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$600.00
MR203043L4 Lenti ORF clone of Mettl7a1 (mGFP-tagged) - Mouse methyltransferase like 7A1 (Mettl7a1) 10 ug
$600.00

file-alt Citations

Single-Cell Analysis of Clonal and Transcriptional Dynamics During Direct Reprogramming
Biddy
Thesis (2025)
AI Summary: OriGene contributed the Mouse Mettl7a1 cDNA clone (MC205948) to this study, which was used for retroviral transduction in comparative reprogramming experiments.
Snippet: Mouse Mettl7a1 (NM 027334, Origene: MC205948) was sub-cloned into the retroviral vector, pGCDNSam16, and retrovirus was produced as described above. For comparative reprogramming experiments, MEFs (1.2×105 cells per 6-cm plate, in 3 independent biological replicates) were serially transduced over 72 h (as above), followed by splitting and seeding at 4×104 cells per well of a 6-well plate to generate technical replicates. …
Single-cell mapping of lineage and identity in direct reprogramming
Biddy, Kong, Kamimoto et al
Nature (2018) 564 (7735), 219-224 DOI: 10.1038/s41586-018-0744-4
AI Summary: OriGene’s Mouse Mettl7a1 cDNA (MC205948) was used in this study to investigate the role of Mettl7a1 in enhancing the efficiency of fibroblast to induced endoderm progenitor reprogramming.
Snippet: Mouse Mettl7a1 (NM 027334, Origene: MC205948) was sub-cloned into the retroviral vector, pGCDNSam16, and retrovirus was produced as described above
Showing 1-2 of 2 papers.
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