Mrto4 (NM_023536) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202929
Mrto4 (tGFP-tagged) - Mouse MRT4 turnover 4, homolog (S. cerevisiae) (Mrto4), mRNA
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrto4
Synonyms 2610012O22Rik; Mg684; Mrt4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202929 representing NM_023536
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAATCCAAGCGAGACAAGAAAGTTTCTTTAACAAAAACTGCCAAGAAAGGCTTGGAACTGAAGC
AGAACCTGATAGAAGAGCTTCGGAAATGTGTGGACACCTACAAGTACCTTTTCATCTTCTCTGTGGCCAA
CATGAGGAACAGCAAGCTGAAAGACATCCGGAACGCCTGGAAACACAGCCGGATGTTCTTTGGCAAAAAC
AAGGTGATGATGGTGGCCTTGGGTCGAAGCCCATCTGATGAATACAAAGACAACCTACATCAGGTCAGCA
AGAAGTTGAGAGGTGAAGTTGGGCTGCTGTTTACCAACCGCACGAAGGAGGAGGTGAATGAGTGGTTCAC
CAAGTATACAGAAATGGATTTTGCTCGAGCGGGGAACAAAGCAACTCTAACTGTGAGCCTGGATCCAGGG
CCCCTGAAGCAATTCCCTCACTCCATGGAGCCACAGCTGAGGCAGCTGGGGCTGCCCACTGCCCTCAAGA
AAGGTGTGGTGACCCTGCTGTCTGACTATGAAGTATGCAAAGAGGGTGACGTGCTGACCCCAGAGCAGGC
CCGTATCCTGCTGTTTGGGTATGAGATGGCTGAATTCAAAGTGATCATCAAATACATGTGGGACGCCCAG
TCAGGACGATTCCAGCAGATGGACGATGACCTGCCTGAGAGCGCGCCTGAGTCTGAGGGAGAGTCTGAGG
AGGAGGATGACAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202929 representing NM_023536
Red=Cloning site Green=Tags(s)

MPKSKRDKKVSLTKTAKKGLELKQNLIEELRKCVDTYKYLFIFSVANMRNSKLKDIRNAWKHSRMFFGKN
KVMMVALGRSPSDEYKDNLHQVSKKLRGEVGLLFTNRTKEEVNEWFTKYTEMDFARAGNKATLTVSLDPG
PLKQFPHSMEPQLRQLGLPTALKKGVVTLLSDYEVCKEGDVLTPEQARILLFGYEMAEFKVIIKYMWDAQ
SGRFQQMDDDLPESAPESEGESEEEDDS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023536
ORF Size 714 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023536.1
RefSeq Size 930 bp
RefSeq ORF 717 bp
Locus ID 69902
UniProt ID Q9D0I8
Cytogenetics 4 D3
Summary Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023536" in other vectors (4)
SKU Description Size Price
MC200549 Mrto4 (untagged) - Mouse MRT4 turnover 4, homolog (S. cerevisiae) (Mrto4), mRNA, (10ug) 10 ug
$300.00
MR202929 Mrto4 (Myc-DDK-tagged) - Mouse MRT4 turnover 4, homolog (S. cerevisiae) (Mrto4), mRNA 10 ug
$289.00 $300.00
MR202929L3 Lenti ORF clone of Mrto4 (Myc-DDK-tagged) - Mouse MRT4 turnover 4, homolog (S. cerevisiae) (Mrto4), mRNA 10 ug
$600.00
MR202929L4 Lenti ORF clone of Mrto4 (mGFP-tagged) - Mouse MRT4 turnover 4, homolog (S. cerevisiae) (Mrto4), mRNA 10 ug
$600.00

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