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Asrgl1 (BC016106) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202818
Asrgl1 (tGFP-tagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Asrgl1 or BC016106"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asrgl1
Synonyms ALP, ALP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202818 representing BC016106
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCTAGTATCATGGATGGGAAGGACCTGTCTGCAGGTGCAGTGTCTGCCGTGCGCTGTATCGCAA
ATCCCGTTAAACTTGCACGGCTTGTTATGGAAAAGACACCTCATTGCTTTCTGACTGGCCATGGTGCAGA
GAAATTTGCAGAAGACATGGGGATTCCACAGGTCCCTGTAGAAAAACTGATAACTGAGAGAACCAAGAAG
CACCTGGAGAAAGAGAAGCTTGAGAAGGGAGCACAGAATGCTGACTGCCCTAAAAACTCAGGAACTGTGG
GTGCTGTTGCCTTGGACTGCAGAGGAAACTTGGCTTACGCAACCTCTACTGGGGGGATTGTCAATAAAAT
GGTTGGCCGAGTTGGAGACTCGCCTTGCATAGGAGCTGGAGGTTACGCAGATAATAACCTTGGAGCCGTT
TCAACCACAGGACATGGGGAAAGTATCCTGAAGGTGAATCTGGCCAGACTTGCCCTCTTCCATGTAGAGC
AAGGAAAGACCGTAGAGGAGGCTGCTCAGTTGGCATTGGATTACATGAAGTCAAAACTCAAAGGTCTAGG
TGGCCTCATCTTGGTCAACAAAACAGGAGACTGGGTGGCAAAGTGGACCTCTGCCTCCATGCCCTGGGCA
GCGGTGAAGAATGGCAAGCTGCAGGCCGGCATTGACCTCTGTGAGACCAGGACAAGGGACCTACCTTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202818 representing BC016106
Red=Cloning site Green=Tags(s)

MDASIMDGKDLSAGAVSAVRCIANPVKLARLVMEKTPHCFLTGHGAEKFAEDMGIPQVPVEKLITERTKK
HLEKEKLEKGAQNADCPKNSGTVGAVALDCRGNLAYATSTGGIVNKMVGRVGDSPCIGAGGYADNNLGAV
STTGHGESILKVNLARLALFHVEQGKTVEEAAQLALDYMKSKLKGLGGLILVNKTGDWVAKWTSASMPWA
AVKNGKLQAGIDLCETRTRDLPC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC016106
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016106, AAH16106
RefSeq Size 1723 bp
RefSeq ORF 701 bp
Locus ID 66514
Cytogenetics 19 A
Summary Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC016106" in other vectors (4)
SKU Description Size Price
MC217824 Asrgl1 (untagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265), (10ug) 10 ug
$330.00
MR202818 Asrgl1 (Myc-DDK-tagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265) 10 ug
$289.00 $300.00
MR202818L3 Lenti ORF clone of Asrgl1 (Myc-DDK-tagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265) 10 ug
$600.00
MR202818L4 Lenti ORF clone of Asrgl1 (mGFP-tagged) - Mouse asparaginase like 1 (cDNA clone MGC:27691 IMAGE:4921265) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.