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Psmc3ip (NM_008949) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202419
Psmc3ip (tGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psmc3ip
Synonyms C79099; GT198; HOP2; Tbpip
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202419 representing NM_008949
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAAAAGCCGGGCCGAGGCTGCGGCTGGAGCCCCCGGGATCATCCTGAGGTACCTGCAAGAACAAA
ACCGGCCCTACAGCGCCCAGGACGTGTTCGGAAACCTACAGAAGGAACATGGACTGGGCAAGGCGGCGGT
AGTGAAGGCGCTGGATCAGCTGGCCCAGGAAGGCAAGATCAAAGAGAAGACCTACGGCAAGCAGAAAATT
TATTTTGCCGATCAGAACCAGTTTGACACAGTGAGTGATGCGGACCTCCACGGCCTGGATGCCAGTATCG
TGGCCCTCACTGCCAAGGTGCAGAGCTTACAGCAGAGCTGCCGACACATGGAGGCCGAGCTGAAGGAATT
AACAAGTGCGCTGACCACTCCTGAGATGCAGAAAGAGATTCAGGAGTTAAAGAAGGAGTGTGCTCAATAC
ACAGAGAGACTGAAGAACATCAAAGCAGCTACCAACCATGTCACTCCAGAAGAGAAGGAGAAGGTGTACA
GAGACAGACAGAAGTACTGTAAAGAGTGGAGGAAGCGGAAGAGGATGACCACAGAGCTGTGTGATGCGAT
CCTTGAAGGCTACCCCAAGAGCAAGAAGCAGTTCTTTGAGGAAGTTGGAATAGAGACAGATGAAGATCAT
AATGTTTTGCTCCCCGACCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202419 representing NM_008949
Red=Cloning site Green=Tags(s)

MSKSRAEAAAGAPGIILRYLQEQNRPYSAQDVFGNLQKEHGLGKAAVVKALDQLAQEGKIKEKTYGKQKI
YFADQNQFDTVSDADLHGLDASIVALTAKVQSLQQSCRHMEAELKELTSALTTPEMQKEIQELKKECAQY
TERLKNIKAATNHVTPEEKEKVYRDRQKYCKEWRKRKRMTTELCDAILEGYPKSKKQFFEEVGIETDEDH
NVLLPDP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008949
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008949.3
RefSeq Size 948 bp
RefSeq ORF 654 bp
Locus ID 19183
UniProt ID O35047
Cytogenetics 11 D
Summary Plays an important role in meiotic recombination. Stimulates DMC1-mediated strand exchange required for pairing homologous chromosomes during meiosis. The complex PSMC3IP/MND1 binds DNA, stimulates the recombinase activity of DMC1 as well as DMC1 D-loop formation from double-strand DNA. This complex stabilizes presynaptic RAD51 and DMC1 filaments formed on single strand DNA to capture double-strand DNA. This complex stimulates both synaptic and presynaptic critical steps in RAD51 and DMC1-promoted homologous pairing. May inhibit HIV-1 viral protein TAT activity and modulate the activity of proteasomes through association with PSMC3.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008949" in other vectors (4)
SKU Description Size Price
MC204035 Psmc3ip (untagged) - Mouse proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip), (10ug) 10 ug
$300.00
MR202419 Psmc3ip (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip) 10 ug
$289.00 $300.00
MR202419L3 Lenti ORF clone of Psmc3ip (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip) 10 ug
$600.00
MR202419L4 Lenti ORF clone of Psmc3ip (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip) 10 ug
$600.00

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