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Sike1 (NM_025679) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202191
Sike1 (tGFP-tagged) - Mouse RIKEN cDNA 5730470L24 gene (5730470L24Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sike1
Synonyms 2810005O12Rik; 5730470L24Rik; AI450236; AI839862; Sike; Sikeb
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202191 representing NM_025679
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGCACGATCGAGAAGATCTTGACGGACGCCAAGACGCTGCTGGAGCGGCTGCGCGAGCACGATG
CGGCGGCCGAGTCGCTGGTGGACCAGTCGGCTGCGCTGCACCGGCGGGTGGCGGCGATGCGGGAGGCGGG
CGCCGTGTTACCCGAGCAGTATCAAGAGGATGCATCCGACGTGAAGGACATGTCTAAATATAAACCTCAC
ATTCTGCTGTCTCAAGAGAATACCCAGATTAGAGACTTACAGCAGGAAAACAGAGAACTGTGGGTTTCCT
TGGAGGAGCACCAGGATGCGTTGGAACTGATCATGAGCAAGTACCGGAAACAGATGTTACAGTTAATGGT
CGCTAAGAAAGCCGTGGATGCCGAACCAGTTCTGAAAGCTCACCAGTCTCACTCTGCAGAGATTGAAAGT
CAGATCGATAGAATCTGTGAAATGGGAGCAGTGATGCGGAGAGCTGTTCAGGTGGACGATAACCAATTCT
GTAAGGTTCAGGAGAGACTAGCTCAGCTGGAGCTTGAAAATAAGGAACTTCGAGAGTTACTGTCCATCAG
CAGTGAATCTCTGCAGGTTGGAAAAGAAAGCTCTGTGGCTCCTGCCTCTCAGACCATCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202191 representing NM_025679
Red=Cloning site Green=Tags(s)

MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGAVLPEQYQEDASDVKDMSKYKPH
ILLSQENTQIRDLQQENRELWVSLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIES
QIDRICEMGAVMRRAVQVDDNQFCKVQERLAQLELENKELRELLSISSESLQVGKESSVAPASQTIK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025679
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025679.3
RefSeq Size 2802 bp
RefSeq ORF 624 bp
Locus ID 66641
UniProt ID Q9CPR7
Cytogenetics 3 F2.2
Summary Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025679" in other vectors (4)
SKU Description Size Price
MC200385 Sike1 (untagged) - Mouse suppressor of IKBKE 1 (Sike1), (10ug) 10 ug
$300.00
MR202191 Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$289.00 $300.00
MR202191L3 Lenti ORF clone of Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$600.00
MR202191L4 Lenti ORF clone of Sike1 (mGFP-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$600.00

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