Sike1 (NM_025679) Mouse Untagged Clone

SKU
MC200385
Sike1 (untagged) - Mouse suppressor of IKBKE 1 (Sike1), (10ug)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sike1
Synonyms 2810005O12Rik; 5730470L24Rik; AI450236; AI839862; Sike; Sikeb
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC004090 sequence for NM_025679
CGGACGCGTGGGGGCCGGGTAAGGTCTGAGCGAAGCGCCTAGGCTAGCGGGCCCGGCGCGCCTCCTCGGC CACTATGAGCTGCACGATCGAGAAGATCTTGACGGACGCCAAGACGCTGCTGGAGCGGCTGCGCGAGCAC GATGCGGCGGCCGAGTCGCTGGTGGACCAGTCGGCTGCGCTGCACCGGCGGGTGGCGGCGATGCGGGAGG CGGGCGCCGTGTTACCCGAGCAGTATCAAGAGGATGCATCCGACGTGAAGGACATGTCTAAATATAAACC TCACATTCTGCTGTCTCAAGAGAATACCCAGATTAGAGACTTACAGCAGGAAAACAGAGAACTGTGGGTT TCCTTGGAGGAGCACCAGGATGCGTTGGAACTGATCATGAGCAAGTACCGGAAACAGATGTTACAGTTAA TGGTCGCTAAGAAAGCCGTGGATGCCGAACCAGTTCTGAAAGCTCACCAGTCTCACTCTGCAGAGATTGA AAGTCAGATCGATAGAATCTGTGAAATGGGAGCAGTGATGCGGAGAGCTGTTCAGGTGGACGATAACCAA TTCTGTAAGGTTCAGGAGAGACTAGCTCAGCTGGAGCTTGAAAATAAGGAACTTCGAGAGTTACTGTCCA TCAGCAGTGAATCTCTGCAGGTTGGAAAAGAAAGCTCTGTGGCTCCTGCCTCTCAGACCATCAAGTAACT GGACTTCCGAAGGCTGGCTACAGCTTGCCCGTCAGAGCGAGAAGCCGTGCCCTCCTCTCCTGCCGTGTCC AAGAGTCCTGCCGCAGACTTGACTTAGTGTTGGGTAGAGGTATGGGGTGGCAGTTTAGAATTGCCACTTG GTATTCATTGTCCACTAACTAGTTTGGGGTGTACTAGTGAAACTAGACAAGTCCCCCATTGCTGGGACAG AGATGACATTTGTCACTCTAGTCCGGAATCTCTATAAACTGTTACCTGCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_025679
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004090, AAH04090
RefSeq Size 992 bp
RefSeq ORF 624 bp
Locus ID 66641
UniProt ID Q9CPR7
Cytogenetics 3 F2.2
Summary Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_025679" in other vectors (4)
SKU Description Size Price
MG202191 Sike1 (tGFP-tagged) - Mouse RIKEN cDNA 5730470L24 gene (5730470L24Rik) 10 ug
$500.00
MR202191 Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$289.00 $300.00
MR202191L3 Lenti ORF clone of Sike1 (Myc-DDK-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$600.00
MR202191L4 Lenti ORF clone of Sike1 (mGFP-tagged) - Mouse suppressor of IKBKE 1 (Sike1) 10 ug
$600.00

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