Cirbp (NM_007705) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201471
Cirbp (tGFP-tagged) - Mouse cold inducible RNA binding protein (Cirbp)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cirbp
Synonyms Cirp; R74941
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201471 representing NM_007705
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCAGATGAAGGCAAGCTTTTCGTGGGAGGACTCAGCTTCGACACCAACGAGCAGGCGCTGGAGC
AGGTCTTCTCCAAGTATGGGCAGATCTCCGAAGTGGTGGTGGTAAAGGACAGGGAGACCCAGCGATCCCG
AGGCTTTGGGTTTGTCACCTTTGAAAATATCGATGACGCTAAGGACGCCATGATGGCTATGAATGGGAAG
TCTGTGGACGGGCGGCAGATCAGAGTTGACCAGGCTGGCAAGTCTTCTGACAACCGGTCCCGAGGATACC
GGGGTGGCTCTGCTGGAGGCCGGGGCTTTTTCCGTGGGGGACGAAGCCGGGGCCGAGGGTTCTCCAGAGG
AGGAGGAGACCGGGGCTATGGAGGTGGCCGCTTTGAGTCCCGGAGTGGGGGTTATGGAGGCTCCAGAGAC
TACTATGCCAGCCGGAGTCAGGGTGGCAGCTATGGTTATCGGAGCTCGGGAGGGTCCTACAGAGACAGCT
ATGACAGTTATGCTACACACAACGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201471 representing NM_007705
Red=Cloning site Green=Tags(s)

MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGK
SVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGYGGGRFESRSGGYGGSRD
YYASRSQGGSYGYRSSGGSYRDSYDSYATHNE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007705
ORF Size 516 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007705.3
RefSeq Size 1256 bp
RefSeq ORF 519 bp
Locus ID 12696
UniProt ID P60824
Cytogenetics 10 39.72 cM
Summary Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3' UTRs) of stress-responsive transcripts RPA2 and TXN.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_007705" in other vectors (4)
SKU Description Size Price
MC203526 Cirbp (untagged) - Mouse cold inducible RNA binding protein (Cirbp), (10ug) 10 ug
$300.00
MR201471 Cirbp (Myc-DDK-tagged) - Mouse cold inducible RNA binding protein (Cirbp) 10 ug
$289.00 $300.00
MR201471L3 Lenti ORF clone of Cirbp (Myc-DDK-tagged) - Mouse cold inducible RNA binding protein (Cirbp) 10 ug
$600.00
MR201471L4 Lenti ORF clone of Cirbp (mGFP-tagged) - Mouse cold inducible RNA binding protein (Cirbp) 10 ug
$600.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.