Lsm3 (NM_026309) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200330
Lsm3 (tGFP-tagged) - Mouse LSM3 homolog, U6 small nuclear RNA associated (S
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lsm3
Synonyms 1010001J12Rik; 2610005D18Rik; 6030401D18Rik; SMX4; USS2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200330 representing NM_026309
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGACGTAGATCAGCAACAGACCACCAATACCGTAGAGGAGCCCCTGGATCTGATCAGGCTCA
GCCTGGATGAGCGAATTTATGTGAAAATGAGAAATGACCGAGAGCTTCGAGGCAGACTACACGCTTATGA
TCAACATTTAAATATGATCCTGGGAGATGTAGAAGAAACTGTGACAACGATAGAGATTGATGAGGAGACA
TATGAAGAGATATATAAATCCACAAAACGAAACATCCCTATGCTCTTCGTCCGGGGAGATGGCGTCGTTC
TAGTCGCCCCTCCATTGAGAGTTGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200330 representing NM_026309
Red=Cloning site Green=Tags(s)

MADDVDQQQTTNTVEEPLDLIRLSLDERIYVKMRNDRELRGRLHAYDQHLNMILGDVEETVTTIEIDEET
YEEIYKSTKRNIPMLFVRGDGVVLVAPPLRVG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026309
ORF Size 306 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026309.2
RefSeq Size 634 bp
RefSeq ORF 309 bp
Locus ID 67678
UniProt ID P62311
Cytogenetics 6 D1
Summary Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026309" in other vectors (4)
SKU Description Size Price
MC202283 Lsm3 (untagged) - Mouse LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm3), (10ug) 10 ug
$150.00
MR200330 Lsm3 (Myc-DDK-tagged) - Mouse LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm3) 10 ug
$289.00
MR200330L3 Lenti ORF clone of Lsm3 (Myc-DDK-tagged) - Mouse LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm3) 10 ug
$450.00
MR200330L4 Lenti ORF clone of Lsm3 (mGFP-tagged) - Mouse LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm3) 10 ug
$450.00

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