OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Ripk1 (NM_009068) Mouse Untagged Clone

Be the first to review this product
SKU
MC220144
Ripk1 (untagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $612.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Ripk1 or NM_009068"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ripk1
Synonyms D330015H01Rik; Rinp; RIP; RIP-1; Rip1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220144 representing NM_009068
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACCAGACATGTCCTTGGACAATATTAAGATGGCATCCAGTGACCTGCTGGAGAAGACAGACCTAG
ACAGCGGAGGCTTCGGGAAGGTGTCCTTGTGTTACCACAGAAGCCATGGATTTGTCATCCTGAAAAAAGT
ATACACAGGGCCCAACCGCGCTGAGTACAATGAGGTTCTCTTGGAAGAGGGGAAGATGATGCACAGACTG
AGACACAGTCGAGTGGTGAAGCTACTGGGCATCATCATAGAAGAAGGGAACTATTCGCTGGTGATGGAGT
ACATGGAGAAGGGCAACCTGATGCACGTGCTAAAGACCCAGATAGATGTCCCACTTTCATTGAAAGGAAG
GATAATCGTGGAGGCCATAGAAGGCATGTGCTACTTACATGACAAAGGTGTGATACACAAGGACCTGAAG
CCTGAGAATATCCTCGTTGATCGTGACTTTCACATTAAGATAGCCGATCTTGGTGTGGCTTCCTTTAAGA
CATGGAGCAAACTGACTAAGGAGAAAGACAACAAGCAGAAAGAAGTGAGCAGCACCACTAAGAAGAACAA
TGGTGGTACCCTTTACTACATGGCACCCGAACACCTGAATGACATCAATGCAAAGCCCACGGAGAAGTCG
GACGTGTACAGCTTTGGCATTGTCCTTTGGGCAATATTTGCAAAAAAGGAGCCCTATGAGAATGTCATCT
GTACTGAGCAGTTCGTGATCTGCATAAAATCTGGGAACAGGCCAAATGTAGAGGAAATCCTTGAGTACTG
TCCAAGGGAGATCATCAGCCTCATGGAGCGGTGCTGGCAGGCGATCCCAGAAGACAGGCCAACATTTCTT
GGCATTGAAGAAGAATTTAGGCCTTTTTACTTAAGTCATTTTGAAGAATATGTAGAAGAGGATGTGGCAA
GTTTAAAGAAAGAGTATCCAGATCAAAGCCCAGTGCTGCAGAGAATGTTTTCACTGCAGCATGACTGTGT
ACCCTTACCTCCGAGCAGGTCAAATTCAGAACAACCTGGATCGCTGCACAGTTCCCAGGGGCTCCAGATG
GGTCCTGTGGAGGAGTCCTGGTTTTCTTCCTCCCCAGAGTACCCACAGGACGAGAATGATCGCAGTGTGC
AGGCTAAGCTGCAAGAGGAAGCCAGCTATCATGCTTTTGGAATATTTGCAGAGAAACAGACAAAACCGCA
GCCAAGGCAGAATGAGGCTTACAACAGAGAGGAGGAAAGGAAACGAAGGGTCTCTCATGACCCCTTTGCA
CAGCAGAGAGCTCGTGAGAATATTAAGAGTGCAGGAGCAAGAGGTCATTCTGATCCCAGCACAACGAGTC
GTGGAATTGCAGTGCAACAGCTGTCATGGCCAGCCACCCAAACAGTTTGGAACAATGGATTGTATAATCA
GCATGGATTTGGAACTACAGGTACAGGAGTTTGGTATCCGCCAAATCTAAGCCAAATGTATAGTACTTAT
AAAACTCCAGTGCCAGAGACCAACATACCGGGAAGCACACCCACCATGCCATACTTCTCTGGGCCAGTAG
CAGATGACCTCATAAAATATACTATATTCAATAGTTCTGGTATTCAGATTGGAAACCACAATTATATGGA
TGTTGGACTGAATTCACAACCACCAAACAATACTTGCAAAGAAGAGTCGACTTCCAGACACCAAGCCATC
TTTGATAACACCACTAGTCTGACTGATGAACACCTGAACCCTATCAGGGAAAACCTGGGAAGGCAGTGGA
AAAACTGTGCCCGCAAGCTGGGCTTCACTGAGTCTCAGATCGATGAAATCGACCATGACTATGAAAGAGA
TGGACTGAAAGAGAAAGTTTACCAAATGCTTCAGAAGTGGCTGATGCGGGAAGGCACCAAAGGGGCCACA
GTGGGAAAGTTGGCCCAGGCACTTCACCAATGTTGCAGGATAGACCTGCTGAACCACTTGATTCGTGCCA
GCCAGAGCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_009068
Insert Size 1971 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009068.3, NP_033094.3
RefSeq Size 4512 bp
RefSeq ORF 1971 bp
Locus ID 19766
UniProt ID Q60855
Cytogenetics 13 14.01 cM
Summary Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:12654725, PubMed:19590578). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (By similarity). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (By similarity). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation (By similarity). Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (By similarity). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009068" in other vectors (6)
SKU Description Size Price
MG209813 Ripk1 (tGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,117.00
MR209813 Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$289.00 $917.00
MR209813L1 Lenti ORF clone of Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00
MR209813L2 Lenti ORF clone of Ripk1 (mGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00
MR209813L3 Lenti ORF clone of Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00
MR209813L4 Lenti ORF clone of Ripk1 (mGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products