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Ripk1 (NM_009068) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR209813L2
Lenti ORF clone of Ripk1 (mGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,217.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ripk1
Synonyms D330015H01Rik; Rinp; RIP; RIP-1; Rip1
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR209813).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009068
ORF Size 1968 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009068.3, NP_033094.3
RefSeq Size 4512 bp
RefSeq ORF 1971 bp
Locus ID 19766
UniProt ID Q60855
Cytogenetics 13 14.01 cM
Summary Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage (PubMed:12654725, PubMed:19590578). Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor (By similarity). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity). Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules (By similarity). Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation (By similarity). Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death (By similarity). RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009068" in other vectors (6)
SKU Description Size Price
MC220144 Ripk1 (untagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1), (10ug) 10 ug
$612.00
MG209813 Ripk1 (tGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,117.00
MR209813 Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$289.00 $917.00
MR209813L1 Lenti ORF clone of Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00
MR209813L3 Lenti ORF clone of Ripk1 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00
MR209813L4 Lenti ORF clone of Ripk1 (mGFP-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 1 (Ripk1) 10 ug
$1,217.00

Plasmid Map for MR209813L2

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209813L2
Circular map for MR209813L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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