Klhl40 (NM_028202) Mouse Untagged Clone

SKU MC219836

Klhl40 (untagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Klhl40
• Synonyms: 2310024D23Rik; Kbtbd5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219836 representing NM_028202; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACGCTGGGCTTGGAGCAGGCGGAGGAACAGCGTCTGTACCAGCAGACGCTCCTGCAAGACGGCCTCA; AGGACATGCTGGACCACGGCAAGTTCCTAGACTGTGTAGTGCGCGTGGGTGAGCGCGAGTTCCCGTGCCA; CCGCCTGGTGCTAGCCGCCTGTAGCCCCTACTTCCGCGCGCGCTTCCTGGCTGAGCCAGATAGCGCGGGA; GAGGTACGCCTGGAGGAGGTGTCGCCAGACGTAGTGTCCCAGGTGCTGCACTACCTGTACACATCAGAGA; TCGCGCTGGATGAGGCAAGCGTGCAAGACCTGTTTGCCGCGGCGCATCGATTCCAGATCCCGTCCATCTT; CACCATCTGCGTGTCGTTTCTGCAGAAGCGCCTGTGCTTGGCCAATTGCCTGGCTGTCTTCCGCCTCGGC; CTCCTGCTGGACTGCGCGCGTCTGGCTGTGGCCGCGCGCGACTTCATCTGCGCGCGCTTCCCGCTGGTGG; CGCGGGACAACGACTTCCTGGGACTCTCGGCTGACGAGCTGATCGCCATCATCTCCAGCGACGGCCTCAA; CGTAGAGAAGGAGGAGGCAGTGTTTGAGGCTGTGATGCGCTGGGCCAGCAGCGGAGATGCTGAGGCGCAG; GCGGAGCGCCAGCGCGCGCTGCCCACGGTCTTCGAGAGCGTTCGCTGCCGCCTGCTGCCTCGTGCTTTCC; TGGAGACTCGCGTGGAGCGCCACCCACTTGTGCGCTCCCAGCCCGAGCTGTTGCGCAAGGTGCAGATGGT; GAAGGATGCACATGAGGGCCGCCTCACTACACTGCGCAAGAAGAAGAAGGAAAAGGGTGAGCAGACAGCT; CGGGCCAAGGAGGCCAACCAGGGCACAGAAGACACCAAGGCTGAGGACGATGAGGAACGAGTACTGCCCG; GGATCCTCAATGACACTCTGCGCTTCGGCATGTTCCTTCAGGATCTCATCTTCATGATCAGCGAGGAAGG; CGCCGTGGCTTACGACCCAGCCGCCAACGAGTGCTACTGTGCATCCCTGTCCACCCAGATCCCCAAGAAC; CATGTCAGTCTGGTGACCAAGGAGAACCAAGTCTTCGTGGCCGGTGGCCTCTTCTACAATGAGGACAACA; AGGAGGACCCTATGAGTGCTTACTTCCTGCAGTTTGACCACTTGGACTCTGAGTGGCTGGGGATGCCGCC; TCTCCCTTCACCTCGCTGCCTCTTCGGCCTGGGGGAGGCTCTCAACGCCATCTACGTGGTCGGCGGCCGG; GAACTCAAGGACAGCGAAGACAGCCTGGACTCAGTCCTGTGCTACGACAGGCTGTCATTCAAATGGGGTG; AGTCAGACCCGCTGCCCTACGCCGTGTACGGCCACACAGTCCTTTCCCACATGGACCTGGTCTATGTCAT; TGGTGGCAAAGGCAAAGACAGGAAATGTCTGAACAAGATGTGTGTCTACGACCCCAAGAAGTTTGAGTGG; AAGGAGCTAGCACCCATGCAGACAGCCCGATCGCTCTTTGGGGCCACGGTCCATGACGGCCGCATCTTTG; TGGCCGCAGGGGTGACAGACACAGGACTTACCAGCTCTTCAGAGGTGTACAGCATTGCAGACAACAAGTG; GACCTCCTTTGAGGCCTTCCCACAGGAACGAAGCTCGCTCAGCCTGGTCAGCCTAGCTGGCACTCTTTAT; GCCTTGGGTGGCTTTGCCACTCTGGAGACGGAGTCTGGAGAGCTGGTCCCCACGGAGCTCAATGACATCT; GGAGATACAACGAGGATGAGAAAAAGTGGGAGGGGGTCCTACGGGAGATCGCCTACGCAGCTGGCGCCAC; CTTCCTCCCTGTGCGCCTCAATGTGCTTCGCCTGACCAAGATGTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028202
• Insert Size: 1866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_028202.3, NP_082478.1
• RefSeq Size: 2455 bp
• RefSeq ORF: 1866 bp
• Locus ID: 72330
• UniProt ID: Q9D783
• Cytogenetics: 9 F4
• Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:25940086). The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (PubMed:25940086). Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (PubMed:24960163).[UniProtKB/Swiss-Prot Function]