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Klhl40 (NM_028202) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216861
Klhl40 (tGFP-tagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $778.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl40
Synonyms 2310024D23Rik; Kbtbd5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216861 representing NM_028202
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCTGGGCTTGGAGCAGGCGGAGGAACAGCGTCTGTACCAGCAGACGCTCCTGCAAGACGGCCTCA
AGGACATGCTGGACCACGGCAAGTTCCTAGACTGTGTAGTGCGCGTGGGTGAGCGCGAGTTCCCGTGCCA
CCGCCTGGTGCTAGCCGCCTGTAGCCCCTACTTCCGCGCGCGCTTCCTGGCTGAGCCAGATAGCGCGGGA
GAGGTACGCCTGGAGGAGGTGTCGCCAGACGTAGTGTCCCAGGTGCTGCACTACCTGTACACATCAGAGA
TCGCGCTGGATGAGGCAAGCGTGCAAGACCTGTTTGCCGCGGCGCATCGATTCCAGATCCCGTCCATCTT
CACCATCTGCGTGTCGTTTCTGCAGAAGCGCCTGTGCTTGGCCAATTGCCTGGCTGTCTTCCGCCTCGGC
CTCCTGCTGGACTGCGCGCGTCTGGCTGTGGCCGCGCGCGACTTCATCTGCGCGCGCTTCCCGCTGGTGG
CGCGGGACAACGACTTCCTGGGACTCTCGGCTGACGAGCTGATCGCCATCATCTCCAGCGACGGCCTCAA
CGTAGAGAAGGAGGAGGCAGTGTTTGAGGCTGTGATGCGCTGGGCCAGCAGCGGAGATGCTGAGGCGCAG
GCGGAGCGCCAGCGCGCGCTGCCCACGGTCTTCGAGAGCGTTCGCTGCCGCCTGCTGCCTCGTGCTTTCC
TGGAGACTCGCGTGGAGCGCCACCCACTTGTGCGCTCCCAGCCCGAGCTGTTGCGCAAGGTGCAGATGGT
GAAGGATGCACATGAGGGCCGCCTCACTACACTGCGCAAGAAGAAGAAGGAAAAGGGTGAGCAGACAGCT
CGGGCCAAGGAGGCCAACCAGGGCACAGAAGACACCAAGGCTGAGGACGATGAGGAACGAGTACTGCCCG
GGATCCTCAATGACACTCTGCGCTTCGGCATGTTCCTTCAGGATCTCATCTTCATGATCAGCGAGGAAGG
CGCCGTGGCTTACGACCCAGCCGCCAACGAGTGCTACTGTGCATCCCTGTCCACCCAGATCCCCAAGAAC
CATGTCAGTCTGGTGACCAAGGAGAACCAAGTCTTCGTGGCCGGTGGCCTCTTCTACAATGAGGACAACA
AGGAGGACCCTATGAGTGCTTACTTCCTGCAGTTTGACCACTTGGACTCTGAGTGGCTGGGGATGCCGCC
TCTCCCTTCACCTCGCTGCCTCTTCGGCCTGGGGGAGGCTCTCAACGCCATCTACGTGGTCGGCGGCCGG
GAACTCAAGGACAGCGAAGACAGCCTGGACTCAGTCCTGTGCTACGACAGGCTGTCATTCAAATGGGGTG
AGTCAGACCCGCTGCCCTACGCCGTGTACGGCCACACAGTCCTTTCCCACATGGACCTGGTCTATGTCAT
TGGTGGCAAAGGCAAAGACAGGAAATGTCTGAACAAGATGTGTGTCTACGACCCCAAGAAGTTTGAGTGG
AAGGAGCTAGCACCCATGCAGACAGCCCGATCGCTCTTTGGGGCCACGGTCCATGACGGCCGCATCTTTG
TGGCCGCAGGGGTGACAGACACAGGACTTACCAGCTCTTCAGAGGTGTACAGCATTGCAGACAACAAGTG
GACCTCCTTTGAGGCCTTCCCACAGGAACGAAGCTCGCTCAGCCTGGTCAGCCTAGCTGGCACTCTTTAT
GCCTTGGGTGGCTTTGCCACTCTGGAGACGGAGTCTGGAGAGCTGGTCCCCACGGAGCTCAATGACATCT
GGAGATACAACGAGGATGAGAAAAAGTGGGAGGGGGTCCTACGGGAGATCGCCTACGCAGCTGGCGCCAC
CTTCCTCCCTGTGCGCCTCAATGTGCTTCGCCTGACCAAGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216861 representing NM_028202
Red=Cloning site Green=Tags(s)

MTLGLEQAEEQRLYQQTLLQDGLKDMLDHGKFLDCVVRVGEREFPCHRLVLAACSPYFRARFLAEPDSAG
EVRLEEVSPDVVSQVLHYLYTSEIALDEASVQDLFAAAHRFQIPSIFTICVSFLQKRLCLANCLAVFRLG
LLLDCARLAVAARDFICARFPLVARDNDFLGLSADELIAIISSDGLNVEKEEAVFEAVMRWASSGDAEAQ
AERQRALPTVFESVRCRLLPRAFLETRVERHPLVRSQPELLRKVQMVKDAHEGRLTTLRKKKKEKGEQTA
RAKEANQGTEDTKAEDDEERVLPGILNDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSTQIPKN
HVSLVTKENQVFVAGGLFYNEDNKEDPMSAYFLQFDHLDSEWLGMPPLPSPRCLFGLGEALNAIYVVGGR
ELKDSEDSLDSVLCYDRLSFKWGESDPLPYAVYGHTVLSHMDLVYVIGGKGKDRKCLNKMCVYDPKKFEW
KELAPMQTARSLFGATVHDGRIFVAAGVTDTGLTSSSEVYSIADNKWTSFEAFPQERSSLSLVSLAGTLY
ALGGFATLETESGELVPTELNDIWRYNEDEKKWEGVLREIAYAAGATFLPVRLNVLRLTKM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028202
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028202.3, NP_082478.1
RefSeq Size 2455 bp
RefSeq ORF 1866 bp
Locus ID 72330
UniProt ID Q9D783
Cytogenetics 9 F4
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:25940086). The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (PubMed:25940086). Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (PubMed:24960163).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028202" in other vectors (4)
SKU Description Size Price
MC219836 Klhl40 (untagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5), (10ug) 10 ug
$637.00
MR216861 Klhl40 (Myc-DDK-tagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5) 10 ug
$289.00 $578.00
MR216861L3 Lenti ORF clone of Klhl40 (Myc-DDK-tagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5) 10 ug
$878.00
MR216861L4 Lenti ORF clone of Klhl40 (mGFP-tagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5) 10 ug
$878.00

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