Rnf152 (NM_178779) Mouse Untagged Clone

SKU MC214454

Rnf152 (untagged) - Mouse ring finger protein 152 (Rnf152), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Rnf152
• Synonyms: A930029B02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214454 representing NM_178779; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGACACTCTCCCAGGATTCCCTGTTGGAATGTCAGATCTGTTTTAATTATTACAGCCCCCGACGAA; GGCCCAAGTTGTTGGATTGCAAGCACACCTGCTGCTCGGTGTGCCTCCAGCAGATGAGGACCAGCCAGAA; GGACGTAAGGTGCCCCTGGTGCCGTGGCATCACCAAATTGCCCCCGGGCTTCTCTGTATCACAACTGCCC; GATGACCCAGAGGTCTTGGCAGTCATTGCCATACCACACACTTCCGAGCATACCCCAGTCTTCATCAAAC; TTCCAAGCAATGGGTGCTACATGCTGCCCCTGCCCATCTCTAAGGAGCGTACACTCCTGCCGGGAGACAT; GGGCTGCCGCCTGCTGCCAGGGAGCCAGCAGAAGTCTCTCACCGTGGTGACCATCCCTGCAGAACAGCAG; CCCCTGCAGGGTGGAGCTCCCCCGGAGGCTGTGGAGGAGGAGCCTGACAGAAGGGGCGTGGTGAAGAGCT; CCACATGGTCTGGCGTGTGCACTGTCATCCTGGTGGCCTGTGTTTTGGTCTTCCTCCTGGGCATTGTGCT; ACACAACATGTCCTGCATCTCTAAGCGCTTCACTGTGATATCCTGTGGTTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178779
• Insert Size: 612 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178779.4, NP_848894.1
• RefSeq Size: 8529 bp
• RefSeq ORF: 612 bp
• Locus ID: 320311
• UniProt ID: Q8BG47
• Cytogenetics: 1 E2.1
• Summary: E3 ubiquitin-protein ligase mediating 'Lys-63'-linked polyubiquitination of RRAGA in response to amino acid starvation. Thereby, regulates mTORC1 signaling and plays a role in the cellular response to amino acid availability (PubMed:25936802). Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.