1500003O03Rik (BC054733) Mouse Untagged Clone

CAT#: MC206143

1500003O03Rik (untagged) - Mouse RIKEN cDNA 1500003O03 gene (cDNA clone MGC:64742 IMAGE:5720240), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 1500003O03Rik
• Synonyms: Sid470p, Chp, Cahp
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC054733
  TCTGCCGCTGCTGCTTCTGGCGCTGCCGCTCCCGGAGGAGCTCCCGTCACGGTGATGGGGTCTCGGGCCT CCACGTTATTGCGGGACGAAGAGCTCGAGGAGATCAAGAAGGAGACTGGCTTTTCCCACAGTCAGATCAC GCGCCTGTACAGCCGCTTCACCAGCCTGGACAAAGGGGAGAACGGGACTCTCAGCCGGGAAGATTTCCAG AGGATTCCAGAACTTGCCATCAACCCACTGGGGGACCGGATCATCAATGCCTTCTTCTCAGAGGGAGAGG ATCAGGTAAACTTCCGAGGATTCATGAGAACATTGGCTCATTTCCGACCCATTGAGGATAACGAAAAGAG CAAAGATGTGAATGGCCCGGAACCCCTCAACAGCCGGAGCAACAAGCTGCACTTTGCTTTCAGACTCTAT GATTTGGATAAAGATGACAAGATCTCCCGTGATGAGCTGTTACAGGTACTGCGAATGATGGTTGGAGTGA ATATCTCGGATGAGCAGCTGGGCAGTATTGCGGACAGGACCATCCAGGAGGCTGACCAAGATGGAGATAG TGCCATATCTTTTACAGAATTCGTTAAGGTTTTGGAAAAAGTGGATGTAGAACAGAAGATGAGCATCCGA TTTCTTCACTAAAGGGCAGAGACCAAATTATTCTTGCTTTCTAGTATTTAAGAACTGAAACTTGTGTCCT CCAGCCCCACCCCATCCTGTCATCCCCTTTTCCCAGAATACTACAGCTCTTGCATGACAACCTGAAGTCT CTCCTGTCCATACAAACTTGCCTTTGGTGTATAAACAGGGCGTTACAGAATGGTACCCTCTGTCTGCTTC TGTTCAGTATCCACCCACCAGGTCCTTAGTTGTCAGTACCATCTTCCCCTGTTAGCTGCTGAGTGCCATG CATCCATCCTGTCTGGGGAAATGAACCAGCCAGCAAAGCTCCCACTGGATGGGGACTATATCCATGCTGC CTTCCATCCCTGCATCGAGCCTCCACCATGTTTCTGTGACCTTGTGTCCTTTTGTCCTTGTGTCTGTTAC TTCCTACCTCTCAGTCATCCAACCTTCTCTCCATTCAGTTTTTCAGCTGCCCTCCTGTCGTTGAGATTCC CTGCATTTTACTTGATTCTGGGCTCTGGGCTCTGCTGTTAAGCCTGTTTCTGATGTGGCAGGATAGTTAG TGGTGTGGTGAGTTATAGCTACCTTCTAATGTGTCTCTGGGACGTTGCCTATCCAGGAGCTTTTCAGCAA GCAGTACTGCTCTTGGAAGCATGTGACTCCCTTGGTCCCCTGTATTGCTCTGTTGTCGAAGGGCTTATGA GCAGACATGTTCTTAAAAGGTGGTCATGGTGAGGCTTGTAGAGCCATGTTTCCTAGGTGGCTTCCCAGGT CTCTCTACCTCTGACTTTTTATTTTCAAACATATTTATGTGGCTTTTCTGGGTACAGTGTTGACATTCAT TAGAAAATATTCTAATGCCTGCCAGTTTGCTTCTCTTTGGAACATTGTCACCACCCAAGTGGCTCTTCTG ACAATGTGGTAGAGGGCCTTCTTGTAGTTTTCTGAACAAGGCTAGGCACCCTGGAGAGCTGGCCACATTT GCTCTACAGCATGGGTCATGCCTCAGTGTTCTGTACACTGGAAGAGTCTTCACATTTCCAAACAGAAAGA CTAGTGTTACAGAAGTGCCTGACTTATCTCAGTCCTAAAGAATTAGATTGTCCTCAGTGTCTTAAAGTTT CGCACAAAACTCTGAATGAATTTTATACTTGGCAAATTTTAATGCTGGACGATGTGCAGTCAGTTCATAG TCCTGGTCCAGGCCTTTAGTGTATTGTCTCATGAGCCTCCTGATGGCGTGACCTTCCTAGCTCCCTGTGT ATACTACGCAGATCCCATAGAAGCATGGGGCCAAGGATTGGTGGCACCAACTGAGGTGACATTTAGAGGT CTTTGAGTGACCCAGCTCAACTTCTCTGCCAATGTCAGCTTGATTTAGATCTCACTGAATCAGGCTGCTT CTGTCCTGTGTCCCTGTGAATCTGCTCTTGGCTGGCTTTCTCTGATGTCGCTTCCTTGCTTGCTTGCTTC GTTGCTTTGGAAGGCTATCCAAGGTGTGTAAACAATTCTTTAGTAAATAAATTGCTATTAACCCAAACAA ACAAAAACACTGCAAAGAGGATGGGTAAAGGTGAGCAACAGGGGAATGGCTGGGATAGATGGCTGGGGCT ACATGAACTGGGGATTCTTTACGAAATATCTGTTAGACTTTGTGATCAAATGATGTGATAAAAGAATTGT ATAAAGGGAATAATTGTCTGATACTGATCTGTTTGAGAAGTATTGAGAAGCTTCTCAATTTAAATATTTA AACTTTCTAAGCTTATGCCCCCTTGGCTGTGTATAGCAAACTGTTTTTATCCACAGTTGTGCCTTATTGT CCCATTAAAGTTGTATTCCTCAATTCATCAATAAATCAATTGTTGTTAAAACAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: BC054733
• Insert Size: 588 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054733, AAH54733
• RefSeq Size: 2536 bp
• RefSeq ORF: 588 bp
• Locus ID: 56398
• Cytogenetics: 2 E5
• Gene Summary: Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membranes. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]