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Kitl (NM_013598) Mouse Untagged Clone

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SKU
MC204279
Kitl (untagged) - Mouse kit ligand (Kitl), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Kitl
Synonyms blz; Clo; Con; contrasted; Gb; Kitlg; Mgf; SCF; SF; Sl; SLF
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC011322
CGAGAGTGCCGCGGGAAAGCAACGGCCAAGGACGGGGCGCTGCGTTCGAGCTACCCAATGCTGGGACTAT CTGCAGCCGCTGCTGGTGCAATATGCTGGAGCTCCAGAACAGCTAAACGGAGTCGCCACACCGCTGCCTG GGCTGGATCGCAGCGCTGCCTTTCCTTATGAAGAAGACACAAACTTGGATTATCACTTGCATTTATCTCC AACTGCTCCTATTTAATCCTCTCGTCAAAACCAAGGAGATCTGCGGGAATCCTGTGACTGATAATGTAAA AGACATTACAAAACTGGTGGCAAATCTTCCAAATGACTATATGATAACCCTCAACTATGTCGCCGGGATG GATGTTTTGCCTAGTCATTGTTGGCTACGAGATATGGTAATACAATTATCACTCAGCTTGACTACTCTTC TGGACAAGTTCTCAAATATTTCTGAAGGCTTGAGTAATTACTCCATCATAGACAAACTTGGGAAAATAGT GGATGACCTCGTGTTATGCATGGAAGAAAACGCACCGAAGAATATAAAAGAATCTCCGAAGAGGCCAGAA ACTAGATCCTTTACTCCTGAAGAATTCTTTAGTATTTTCAATAGATCCATTGATGCCTTTAAGGACTTTA TGGTGGCATCTGACACTAGTGACTGTGTGCTCTCTTCAACATTAGGTCCCGAGAAAGATTCCAGAGTCAG TGTCACAAAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGTAAT AGGAAAGCTGCAAAGTCCCCTGAAGACTCGGGCCTACAATGGACAGCCATGGCATTGCCGGCTCTCATTT CGCTTGTAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAAACAGTCAAGTCTTACAAGGGCAGT TGAAAATATACAGATTAATGAAGAGGATAATGAGATAAGTATGTTGCAACAGAAAGAGAGAGAATTTCAA GAGGTGTAATTGTGGACGTATCAACATTGTTACCTTCGCACAGTGGCTGGTAACAGTTCATGTTTGCTTC ATAAATGAAGCAGCCTTAAACAAATTCCCATTCTGTCTCAAGTGACAGACCTCACCCTTACCTGTTCTTG CTACCCGTGACCTTGTGTGGATGATTCAGATGTTGGAGCAGAGTGCTTCGCTGTGAACCCTGCACTGAAT TATCATCTGTAAAGAAAAATCTGCACGGAGCAGGACTCTGGAGGTTTTGCAAGTGATGATAGGGACAAGA ACATGTGTCCAGTCTACTTGCACCGTTTGCATGGCTTGGGAAATGTCTGAGTGCTGAAAACCCACCCAGC TTTGTTCTTCAGTCACAACCTGCAGCCTGTCGTTAATTATGGTCTCTGCAAGTAGATTTCAGCCTGGATG GTGGGGGGAATTTTTTTTTCACAAAGGGATGTAGAAAACAATTTTAAAAAAACAAAACAAAACAATAACT CCGTAGCATCAGCCACTGTATGGAGTTGAAAGCCATGGGGAGTATTTTTTTTTTTCTTTTGAGCAGGCTC ATCCGCTCTGTAGTTAATGATTAAGGAGAGCAGCCTAAATGTAAGCTCCTCCTGACAGAAGTCATGGTGC ACCGTATCCTACTAGAGAAATGGACATGGGTAGCTAGTTCTATCCATGCGGTGGAGCCCATGCTGAACTG CAGCATAATACCACGGCATATGGAAGACTCTCCAGAAAAGTCCTTACAGGAGAAAATTGTCAACGTGGAC CAGTGGAAGAACATGCCAAGGAAGACATTTGGCCCATCGTGAAGCCAGTCTTTTCCCTTGACAGTCACTC TAGCGTGTGTAAATCGTGTTTGCAATAGTATAATGTGACATGCTTATGTTTTGAAACTACTGCAGCGGTA TGAAGACAGATATGGGAGGAATGCTGAATGTGCAAGGGTCTCCCCACCCCCACCCCCCTTTTATTTTAAA TCCTGATGGGAGAGAATAAGAACGGATGATGGTATTTAGTACCTAAGATTAGATGCGAGTACTTTATTCT AATACGGGAGTTGCAAACTAAGTCACTCATATTCTAAAAGAATGCAAGGACTCGGAAAACATTAGTTATT TGCAGTATAACCGTTATCTCTTGAAACATAGCCACTTATTTAAATCGGGCACTCTGTATTCATTAAGGTT TATGAAACGAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_013598
Insert Size 822 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011322, AAH11322
RefSeq Size 2192 bp
RefSeq ORF 822 bp
Locus ID 17311
UniProt ID P20826
Cytogenetics 10 51.4 cM
Summary Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
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"NM_013598" in other vectors (4)
SKU Description Size Price
MG203650 Kitl (tGFP-tagged) - Mouse kit ligand (Kitl) 10 ug
$500.00
MR203650 Kitl (Myc-DDK-tagged) - Mouse kit ligand (Kitl) 10 ug
$289.00 $300.00
MR203650L3 Lenti ORF clone of Kitl (Myc-DDK-tagged) - Mouse kit ligand (Kitl) 10 ug
$600.00
MR203650L4 Lenti ORF clone of Kitl (mGFP-tagged) - Mouse kit ligand (Kitl) 10 ug
$600.00

file-alt Citations

Kit Ligand and Kit receptor tyrosine kinase sustain synaptic inhibition of Purkinje cells
Zaman, Vogt, Prokop et al
Elife (2024) 12 DOI: 10.7554/eLife.89792
AI Summary: OriGene’s mouse Kitl cDNA (MC204279) was used to generate transgenic constructs for functional studies of Kit Ligand in this paper.
Snippet: d to express transgenes under the hUbiC promoter and pseudotyped with VSV-G (Luikart et al., 2011;Williams et al., 2015). Silent mutations disrupted the internal EcoRI sites of mouse Kitl (MC204279, OriGene), the Kitl coding sequence was then flanked by EcoRI sites, and the subsequent Kitl coding sequence was introduced into a FUC-T2A-(EcoRI) plasmid, to create a FUC-T2A-KL-1 plasmid, wherein C d
FigureFigureFigure
Showing 1-1 of 1 papers.
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