Kitl (NM_013598) Mouse Untagged Clone

SKU MC204279

Kitl (untagged) - Mouse kit ligand (Kitl), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Kitl
• Synonyms: blz; Clo; Con; contrasted; Gb; Kitlg; Mgf; SCF; SF; Sl; SLF
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011322; CGAGAGTGCCGCGGGAAAGCAACGGCCAAGGACGGGGCGCTGCGTTCGAGCTACCCAATGCTGGGACTAT CTGCAGCCGCTGCTGGTGCAATATGCTGGAGCTCCAGAACAGCTAAACGGAGTCGCCACACCGCTGCCTG GGCTGGATCGCAGCGCTGCCTTTCCTTATGAAGAAGACACAAACTTGGATTATCACTTGCATTTATCTCC AACTGCTCCTATTTAATCCTCTCGTCAAAACCAAGGAGATCTGCGGGAATCCTGTGACTGATAATGTAAA AGACATTACAAAACTGGTGGCAAATCTTCCAAATGACTATATGATAACCCTCAACTATGTCGCCGGGATG GATGTTTTGCCTAGTCATTGTTGGCTACGAGATATGGTAATACAATTATCACTCAGCTTGACTACTCTTC TGGACAAGTTCTCAAATATTTCTGAAGGCTTGAGTAATTACTCCATCATAGACAAACTTGGGAAAATAGT GGATGACCTCGTGTTATGCATGGAAGAAAACGCACCGAAGAATATAAAAGAATCTCCGAAGAGGCCAGAA ACTAGATCCTTTACTCCTGAAGAATTCTTTAGTATTTTCAATAGATCCATTGATGCCTTTAAGGACTTTA TGGTGGCATCTGACACTAGTGACTGTGTGCTCTCTTCAACATTAGGTCCCGAGAAAGATTCCAGAGTCAG TGTCACAAAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGTAAT AGGAAAGCTGCAAAGTCCCCTGAAGACTCGGGCCTACAATGGACAGCCATGGCATTGCCGGCTCTCATTT CGCTTGTAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAAACAGTCAAGTCTTACAAGGGCAGT TGAAAATATACAGATTAATGAAGAGGATAATGAGATAAGTATGTTGCAACAGAAAGAGAGAGAATTTCAA GAGGTGTAATTGTGGACGTATCAACATTGTTACCTTCGCACAGTGGCTGGTAACAGTTCATGTTTGCTTC ATAAATGAAGCAGCCTTAAACAAATTCCCATTCTGTCTCAAGTGACAGACCTCACCCTTACCTGTTCTTG CTACCCGTGACCTTGTGTGGATGATTCAGATGTTGGAGCAGAGTGCTTCGCTGTGAACCCTGCACTGAAT TATCATCTGTAAAGAAAAATCTGCACGGAGCAGGACTCTGGAGGTTTTGCAAGTGATGATAGGGACAAGA ACATGTGTCCAGTCTACTTGCACCGTTTGCATGGCTTGGGAAATGTCTGAGTGCTGAAAACCCACCCAGC TTTGTTCTTCAGTCACAACCTGCAGCCTGTCGTTAATTATGGTCTCTGCAAGTAGATTTCAGCCTGGATG GTGGGGGGAATTTTTTTTTCACAAAGGGATGTAGAAAACAATTTTAAAAAAACAAAACAAAACAATAACT CCGTAGCATCAGCCACTGTATGGAGTTGAAAGCCATGGGGAGTATTTTTTTTTTTCTTTTGAGCAGGCTC ATCCGCTCTGTAGTTAATGATTAAGGAGAGCAGCCTAAATGTAAGCTCCTCCTGACAGAAGTCATGGTGC ACCGTATCCTACTAGAGAAATGGACATGGGTAGCTAGTTCTATCCATGCGGTGGAGCCCATGCTGAACTG CAGCATAATACCACGGCATATGGAAGACTCTCCAGAAAAGTCCTTACAGGAGAAAATTGTCAACGTGGAC CAGTGGAAGAACATGCCAAGGAAGACATTTGGCCCATCGTGAAGCCAGTCTTTTCCCTTGACAGTCACTC TAGCGTGTGTAAATCGTGTTTGCAATAGTATAATGTGACATGCTTATGTTTTGAAACTACTGCAGCGGTA TGAAGACAGATATGGGAGGAATGCTGAATGTGCAAGGGTCTCCCCACCCCCACCCCCCTTTTATTTTAAA TCCTGATGGGAGAGAATAAGAACGGATGATGGTATTTAGTACCTAAGATTAGATGCGAGTACTTTATTCT AATACGGGAGTTGCAAACTAAGTCACTCATATTCTAAAAGAATGCAAGGACTCGGAAAACATTAGTTATT TGCAGTATAACCGTTATCTCTTGAAACATAGCCACTTATTTAAATCGGGCACTCTGTATTCATTAAGGTT TATGAAACGAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013598
• Insert Size: 822 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC011322, AAH11322
• RefSeq Size: 2192 bp
• RefSeq ORF: 822 bp
• Locus ID: 17311
• UniProt ID: P20826
• Cytogenetics: 10 51.4 cM
• Summary: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).