Kitl (NM_013598) Mouse Tagged ORF Clone

SKU MR203650

Kitl (Myc-DDK-tagged) - Mouse kit ligand (Kitl)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Kitl
• Synonyms: blz; Clo; Con; contrasted; Gb; Kitlg; Mgf; SCF; SF; Sl; SLF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203650 representing NM_013598; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGAAGACACAAACTTGGATTATCACTTGCATTTATCTCCAACTGCTCCTATTTAATCCTCTCGTCA; AAACCAAGGAGATCTGCGGGAATCCTGTGACTGATAATGTAAAAGACATTACAAAACTGGTGGCAAATCT; TCCAAATGACTATATGATAACCCTCAACTATGTCGCCGGGATGGATGTTTTGCCTAGTCATTGTTGGCTA; CGAGATATGGTAATACAATTATCACTCAGCTTGACTACTCTTCTGGACAAGTTCTCAAATATTTCTGAAG; GCTTGAGTAATTACTCCATCATAGACAAACTTGGGAAAATAGTGGATGACCTCGTGTTATGCATGGAAGA; AAACGCACCGAAGAATATAAAAGAATCTCCGAAGAGGCCAGAAACTAGATCCTTTACTCCTGAAGAATTC; TTTAGTATTTTCAATAGATCCATTGATGCCTTTAAGGACTTTATGGTGGCATCTGACACTAGTGACTGTG; TGCTCTCTTCAACATTAGGTCCCGAGAAAGATTCCAGAGTCAGTGTCACAAAACCATTTATGTTACCCCC; TGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGTAATAGGAAAGCTGCAAAGTCCCCTGAAGAC; TCGGGCCTACAATGGACAGCCATGGCATTGCCGGCTCTCATTTCGCTTGTAATTGGCTTTGCTTTTGGAG; CCTTATACTGGAAGAAGAAACAGTCAAGTCTTACAAGGGCAGTTGAAAATATACAGATTAATGAAGAGGA; TAATGAGATAAGTATGTTGCAACAGAAAGAGAGAGAATTTCAAGAGGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203650 representing NM_013598; Red=Cloning site Green=Tags(s); ; MKKTQTWIITCIYLQLLLFNPLVKTKEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWL; RDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEF; FSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKAAKSPED; SGLQWTAMALPALISLVIGFAFGALYWKKKQSSLTRAVENIQINEEDNEISMLQQKEREFQEV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013598
• ORF Size: 819 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013598.1, NM_013598.2, NM_013598.3, NP_038626.1
• RefSeq Size: 5449 bp
• RefSeq ORF: 822 bp
• Locus ID: 17311
• UniProt ID: P20826
• Cytogenetics: 10 51.4 cM
• MW: 31.1 kDa
• Summary: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.[UniProtKB/Swiss-Prot Function]