Rnf11 (NM_013876) Mouse Untagged Clone

SKU MC203728

Rnf11 (untagged) - Mouse ring finger protein 11 (Rnf11), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Rnf11
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC010299; GTGGGGAAGCGCTCAGTCTTCCCGCCCCCGCCCCCCTCAGGTTCCAGACGCGACCCCGGCGCAGCCCGCG GCCTAGCCTCGCGCCCCCTGCCCCGGGGGCTGGCATGAGCGGCCCCTCGGTGGCGCCGGGGCGCGGGGGA GCCGCCGCCTGAGCCCCGGGCCTGCCGCCTTGGACCCCGCGGCGCCGAGCGAGCTCACCGCCGAGCGTGC GAGAGACCGGGTCACAGCTTTCTTCCTCCCGCAGATCTCAAGCATACACCCCAGCCCCGGAAGATGGGGA ACTGCCTCAAATCCCCGACTTCGGATGACATCTCCCTGCTTCACGAGTCTCAGTCCGACCGGGCTAGCTT TGGCGAGGGGACGGAGCCAGATCAGGAGCCGCCGCCGCCATATCAGGAACAAGTTCCTGTTCCCATCTAT CATCCGACACCTAGCCAGACTCGGCTAGCAACTCAGCTGACTGAAGAGGAACAAATTAGAATAGCTCAAA GAATAGGCCTTATACAGCATCTGCCTAAAGGAGTTTATGACCCTGGAAGAGATGGATCAGAAAAAAAGAT CCGAGAGTGTGTGATCTGTATGATGGACTTTGTTTATGGGGACCCAATTCGATTTCTGCCGTGCATGCAC ATCTATCACCTGGACTGTATAGATGACTGGTTGATGAGATCCTTCACGTGCCCCTCCTGCATGGAGCCAG TGGATGCAGCACTGCTTTCATCCTATGAGACTAATTGAGCCAGGGTCTCTCGTCTGACTTCAAGTGAACC ATCGGTTTTGGTGGTTTTGATCTTTTGTCACTGAGCCCAAAGAGCCAGGGATTAGGAATTAAGATCATGC ACAAAAGTTTCCTAAAAATTCCTGGATGGCTGCAGATGTTGAGGGAAGTATGTGATATTTTAGAAATTTA GGGGGAAAGTAGGATGGATGGTATTTTTATGTAAAGCCTTGACCCAGTGTTTAAAATATAATTGTTATTA GACCTTGTTCTTGCTCCAGTACATAGGAATTGTGTAAAGTGTTACAGCAACTGTACATGTTTAAATTGTG TGTGTTGAAGCTTAGGAAAAAGATAGTTGTTATTTTTCCTAAATGAAATAAGTTTCTTCTCCCACCCAAA TTCTTTTCTGAAAAGTTACTGACATTTGGGTCAAGGTTTTATTAAAAGCTACATTTTATAACACTGGCAC AAAAAAAAAAAAATAGTAGTTTTAAGCTTGTTTGCACAGTTCTTTTTTTCCATTGGAAATGGAATTCATT GCCTTAGGTCTTTTTAAATAGTGTATTATTATCGTTGGGGCTGGCTCTATGCTTGAAAACCAGTTTATTT ATAACCTGTTGTAAGTGCTATATTCTGTTTGCAGTTAGGAAATGCAGAATTCACAGTGATCTCCTAGCTT GTAAGCAAACTGAGATGCACTATCCCTTTTCTATTAAGAATGAGTTGATGTGTCAGGAAACAAGGCTAGC AAAGTGGATTTGATATCCCTTTCCTTGTGTGTCTTATCTTATTGTTTTGGTGGTTAATATGGTGATAATT GAAAGTCAAGTTGAAATTCAAATATTAATATTTCTGATTTGTTGAGCTTTGAATTATGCCACCATGCTTA TATAAAAACGAAGGTGGCACCATGATGAAACTTAAAGAAACAGTTTCTCATCTGTAACAGTTTTGATTCT CTTTCCTGTGACCTCTTTCCCCACTGTCTGGAACCATAGCAAAAGGATACTGCATCTCTTGTTATTGTAG TGCTGAGGTTATTGAAGTTACAGAAACACATCTGGTGTGTTTCCTAGAAAAGTACCTGTTCAGTCTTGCT AGGTGACTGACAGGAGAGGCAAATGTGTTATGTTTTGCACACCAATGCAGCAGGGTTTGTATAACCACAT GACTTCATAGTTGTGATCTCAAAGGAACTTCTCCTTTATTTTTTCTTGCAATTAATGTAAGAATACTCTA AATCTCTGCACTTCTGAAGGATTAGAGGTAGAAATGGTCTAGTAAAGTGTAATGTTTTATTCATTTAGCA TGTGTTTATTTTCCTTATGTTCTCCAGGTGATTCGTTCCTTCCACTCAGTGATATTGTGGTTAAAAAATG ATGAGCAGAAGCGCTCTCCACCTTACAAATGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013876
• Insert Size: 465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC010299, AAH10299
• RefSeq Size: 2147 bp
• RefSeq ORF: 465 bp
• Locus ID: 29864
• UniProt ID: Q9QYK7
• Cytogenetics: 4 C7
• Summary: Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.[UniProtKB/Swiss-Prot Function]