Map1lc3b (NM_026160) Mouse Untagged Clone

SKU MC203443

Map1lc3b (untagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Map1lc3b
• Synonyms: 1010001C15Rik; Atg8; LC3b; Map1lc3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068180; CACACAAGGGAAGTGATCGTCGCCGGAGTCAGATCGTCTGGCTCGGGACCCCCGCGCGCCATGCCGTCCG AGAAGACCTTCAAGCAGCGCCGGAGCTTTGAACAAAGAGTGGAAGATGTCCGGCTCATCCGGGAGCAGCA CCCCACCAAGATCCCAGTGATTATAGAGCGATACAAGGGGGAGAAGCAGCTGCCCGTCCTGGACAAGACC AAGTTCCTGGTGCCTGACCACGTGAACATGAGCGAGCTCATCAAGATAATCAGACGGCGCTTGCAGCTCA ATGCTAACCAAGCCTTCTTCCTCCTGGTGAATGGGCACAGCATGGTGAGTGTGTCCACTCCCATCTCCGA AGTGTACGAGAGTGAGAGAGATGAAGACGGCTTCCTGTACATGGTTTATGCCTCGCAGGAGACATTCGGG ACAGCAATGGCTGTGTAAGACTCCAACAAAGCCAATGGTTGTTAAGCCCTTACCAAGGCAAAAAGGGATG TTACCAGCGGACGCTGGACGGCTCACCACCCACAGATGAGAACGTAGGCACCCACATAGGGTATTAGGAA CTGTTCATCAGCCAGAAACTGAGCTCCATGCAAGTGCACTCAGCTTGGAAACTCGTCTAAACTAGGCTAT TTTGTGTTCAAACTTTAGAAGTTAAAAATAAAATACTTTTTGCATCCTAAGTTGCCAATAAAAAGATCAA GTTATTTTGACATTTTTCTCCCTAGTAGGGACTTAAAACAGAAGAGCAGTGTCAGGGGCAGTCTCTTCCT GGGACCTGCTGCCTTCTCTAAAAGGGCTCCCACTCAGCAGAGAAGCCCCTCTGAAGGCCCAGGTTGCCTA GCAGAGGCCCCCAGAGGGCCCTGGCTTTGCCCTAAGCAGGTCTGGTGTTGTAGGATATAGCTCTAAGCCG GGTCCTGGTCTTGACCCCAGCCACACCCTTTCACTCAGCAGTGCAGGGACATGCAGCTCGCTGCTGTCTA GATGTTACCATACGCCCTTCTGCTGAGGCCAGGTCTCCGCTTGTCCAGGGGTTGCTGACCCAGCTTAAGC GACTGGAGAGCTGTTTCTCTCCTGGTTTGAATTCTGTCTGGTCCATGCCTGCTTCAGGGAGAGCAAGCGC TGGCCTTAGGATGTGGTTGTCAAGTGGTAGACTGCAAGTCCAATGCTCCAGACTTGGCTCACAGCTCTTT GTTGGTGTGTAACTGTCTCTGTAAGGGTGGTTCTGACAGCTGTTGGGATTTCCCCATTTCATGTCAGTAC ATACATGTCAGCTGTGAGGACAACAGCAACAGAAGACATGCTTTTGATAGACATGCCACTTCAGCTAAAG CTAGCCTCTGCTTCCTGCATGTGGCCCGCTGTGACACCAGGCCATCCCTGGTCACTCACTCACTCGTGGT CTAAGGATCTCAGAGCTGCCTGCTGTCATGGACTGAAGCCAGCATAGCCACCTCCCACCTAGCTCCCCAC AGGCACCCACCTTCCCTGCATGCAGCTGTCTTTGCTAACCCTCAATGTTATTTTACACTGTAAATTCTCA CTGCTCTGTCTTGTGTAGGTTGTATACGTCATTATGTCCCTGGTTTTTATAACTATGGTGCGATCAGTAA GGATTCCTGTAATACTGCTTTAAAGATGCTGCTCAGAGGGCGGGCGGCCTCCATTATAGGACACCTGTAC ACTCTGATGCACTAATAAAGGCACAACGAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_026160
• Insert Size: 378 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC068180, AAH68180
• RefSeq Size: 1742 bp
• RefSeq ORF: 378 bp
• Locus ID: 67443
• UniProt ID: Q9CQV6
• Cytogenetics: 8 E1
• Summary: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.[UniProtKB/Swiss-Prot Function]